A grass carp model with an antibiotic-disrupted intestinal microbiota

Sun, Bing-Yao; Yang, Huixing; He, Wen; Tian, Danyang; Kou, Hai-Yan; Wu, Kang; Yang, Chul-Ju; Cheng, Zhongqin; Song, Xiaofeng

doi:10.1016/j.aquaculture.2021.736790

Cited by 27 publications

(8 citation statements)

References 72 publications

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“…Proteobacteria are considered a signature of dysbiosis in the gut microbiota [85]. The disruption feature of gut microbiota in the antibiotic-induced grass carp model is a significant expansion of Proteobacteria [86]. The CTX exposure in water may disrupt the anaerobic environment of the gut [87] in A. sinica, which leads to the overabundance of Proteobacteria.…”

Section: Discussionmentioning

confidence: 99%

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Pang,

Zheng,

Wang

et al. 2024

Microorganisms

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Cefotaxime (CTX) is an easily detectable antibiotic pollutant in the water environment, but little is known about its toxic effects on aquatic invertebrates, especially on the intestine. Here, we determined the oxidative stress conditions of A. sinica under CTX exposure with five concentrations (0, 0.001, 0.01, 0.1 and 1 mg/L) for 14 days. After that, we focused on changes in intestinal tissue morphology and gut microbiota in A. sinica caused by CTX exposure at 0.01 mg/L. We found malondialdehyde (MDA) was elevated in CTX treatment groups, suggesting the obvious antibiotic-induced oxidative stress. We also found CTX exposure at 0.01 mg/L decreased the villus height and muscularis thickness in gut tissue. The 16S rRNA gene analysis indicated that CTX exposure reshaped the gut microbiota diversity and community composition. Proteobacteria, Actinobacteriota and Bacteroidota were the most widely represented phyla in A. sinica gut. The exposure to CTX led to the absence of Verrucomicrobia in dominant phyla and an increase in Bacteroidota abundance. At the genus level, eleven genera with an abundance greater than 0.1% exhibited statistically significant differences among groups. Furthermore, changes in gut microbiota composition were accompanied by modifications in gut microbiota functions, with an up-regulation in amino acid and drug metabolism functions and a down-regulation in xenobiotic biodegradation and lipid metabolism-related functions under CTX exposure. Overall, our study enhances our understanding of the intestinal damage and microbiota disorder caused by the cefotaxime pollutant in aquatic invertebrates, which would provide guidance for healthy aquaculture.

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Section: Discussionmentioning

confidence: 99%

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Pang,

Zheng,

Wang

et al. 2024

Microorganisms

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“…Many aspects of ectotherm physiology can be affected by environmental temperature ( Angilletta et al, 2002 ), and thus microbial community structure can be known to influence certain physical functions. Indeed, many studies have shown that the immunity of fish could be affected by temperature ( Collazos et al, 1994 ; Alcorn et al, 2002 ; Nikoskelainen et al, 2004 ; Abram et al, 2017 ; Rem, 2019 ) and the immunity response of fish can be regulated by intestinal microbiota ( Xiong et al, 2020 ; Sun et al, 2021 ). Additionally, it has been discovered that gut microbiota has effects on behavior, growth and digestive system of fish ( Bairagi et al, 2002 ; Ray et al, 2012 ; Butt and Volkoff, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota of two invasive fishes respond differently to temperature

Zhang

Yang

et al. 2023

Front. Microbiol.

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Temperature variation structures the composition and diversity of gut microbiomes in ectothermic animals, key regulators of host physiology, with potential benefit to host or lead to converse results (i.e., negative). So, the significance of either effect may largely depend on the length of time exposed to extreme temperatures and how rapidly the gut microbiota can be altered by change in temperature. However, the temporal effects of temperature on gut microbiota have rarely been clarified. To understand this issue, we exposed two juvenile fishes (Cyprinus carpio and Micropterus salmoides), which both ranked among the 100 worst invasive alien species in the world, to increased environmental temperature and sampled of the gut microbiota at multiple time points after exposure so as to determine when differences in these communities become detectable. Further, how temperature affects the composition and function of microbiota was examined by comparing predicted metagenomic profiles of gut microbiota between treatment groups at the final time point of the experiment. The gut microbiota of C. carpio was more plastic than those of M. salmoides. Specifically, communities of C. carpio were greatly altered by increased temperature within 1 week, while communities of M. salmoides exhibit no significant changes. Further, we identified 10 predicted bacterial functional pathways in C. carpio that were temperature-dependent, while none functional pathways in M. salmoides was found to be temperature-dependent. Thus, the gut microbiota of C. carpio was more sensitive to temperature changes and their functional pathways were significantly changed after temperature treatment. These results showed the gut microbiota of the two invasive fishes differ in response to temperature change, which may indicate that they differ in colonization modes. Broadly, we have confirmed that the increased short-term fluctuations in temperatures are always expected to alter the gut microbiota of ectothermic vertebrates when facing global climate change.

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“…According to a study by Sun et al (2021), an antibiotic cocktail (vancomycin, enrofloxacin, florfenicol, and metronidazole) alternate the microbial community structure within the intestinal mucosal and luminal niches in grass carp.…”

Section: B Antibioticsmentioning

confidence: 99%

Bacterial and microalgal communities in carp polyculture systems: Composition, affecting factors and further perspectives.

Ngan¹,

Heyse²,

Delamare-Deboutteville³

et al. 2023

Preprint

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Carp polyculture is the most widely practiced fish production system on the planet, using multiple fish species living in diverse trophic and spatial niches of a pond to maximize productivity. Microorganisms play an important role in pond ecosystems. A variety of new molecular techniques, including 16S/18S rRNA gene sequencing, metatranscriptomics, and metagenomics provide an opportunity for a more thorough understanding of carp polyculture microbiota, and with that, the opportunity to dramatically improve farming practices. In order to support the development of improved best management practices for carp polyculture, here, we reviewed the current knowledge of microbiota in carp polyculture systems (focusing on bacteria and microalgae). The review highlights the diversity and functionality of the microbiota (including bacteria and microalgae) that affect the fish and the complex biotic and abiotic interactions that impact the microbiota of the water in which they grow. The effects of inputs such as probiotics, antibiotics, fertilizers, feeds and environmental parameters on microbial communities were also summarized. Finally, we identified important knowledge gaps and further perspectives regarding microbiota in carp polyculture systems.KEYWORDS: bacteria, microalgae, microbiota, carp polyculture.

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A grass carp model with an antibiotic-disrupted intestinal microbiota

Cited by 27 publications

References 72 publications

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Gut microbiota of two invasive fishes respond differently to temperature

Bacterial and microalgal communities in carp polyculture systems: Composition, affecting factors and further perspectives.

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