A green chemistry approach for nanoencapsulation of bioactive compound – Curcumin

Rao, P. Jagan Mohan; Khanum, Hafeeza

doi:10.1016/j.lwt.2015.08.070

Cited by 55 publications

(17 citation statements)

References 37 publications

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“…Clove oil loaded nanoemulsions (CON) were prepared using oil‐in‐water emulsification method (Rao & Khanum, 2016) followed by ultrasonication with 60% amplitude (Sonics Ultrasonic processor, model no VCX750) for 30 min. The maltodextrin, 4% (wt/vol) used as a carrier, was homogenized in 100 ml TD water followed by addition of 0.75% (vol/vol) clove oil and 60% Tween‐20/40/80 (by volume).…”

Section: Methodsmentioning

confidence: 99%

Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

Nagaraju

Sengupta

Chicgovinda

et al. 2021

J Food Process Engineering

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The therapeutic properties of clove oil are well known, however, the pungent nature, chemical instability, and low water solubility impose limitations in harnessing its potential. Nanoemulsion of clove oil (CON) was prepared to overcome the above constraints. The non-ionic surfactants Tween 20, 40, and 80 with hydrophile lipophile balance (HLB) of 16.7, 15.6, and 15, respectively, were used to stabilize CON. The creaming index of CON prepared with Tween 20, 40, and 80 was 22.75, 17.5, and 1.5%, respectively, after 8 days of storage at room temperature. Tween 20 and 40 produced particles >300 nm while Tween 80 produced 150 nm. Transmission electron microscopic image of spray-dried CON (with Tween 80) showed particles in the range of 150-190 nm. The in vitro release studies showed 76 and 42% cumulative release of CON and native clove oil (NC), respectively, at pH 7.4. The cellular toxicity of CON was significantly reduced by fourfold compared to NC at a concentration of 60 μg/ml when tested on CaCO 2 cells. Similarly, hemolytic activity on red blood cells revealed less than 10% hemolysis. In addition, CON also exhibited higher in-vitro antioxidant compared to NC as shown by 1, 1-diphenylpicrylhydrazyl and 2,2 0-azino-di-[3-ethylbenzthiazoline sulfonate (6)] (ABTS) radical scavenging activity. Practical application: The study highlights the development of clove oil nanoemulsion using cost-effective, commercially important polysaccharide (maltodextrin) and surfactant for increased bioactivity. The spray-dried nanoparticle showed high retention (95%) of oil and small particle size even after 1 month of storage at room temperature. The biological studies signify the compatibility of nanoparticle for its nutraceutical applications because of its low cytotoxicity (fourfold reduction), hemolytic activity, and high anti-oxidant property. The clove oil nanoparticles can replace the clove oil/microencapsulated particles in food product development due to their high water dispersibility and loading content (25% on dry weight basis). 1 | INTRODUCTION Clove oil, an essential oil extracted from the spice Syzigum aromaticaum, is known for its therapeutic properties such as antioxi

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Section: Methodsmentioning

confidence: 99%

Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

Nagaraju

Sengupta

Chicgovinda

et al. 2021

J Food Process Engineering

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“…It is highly likely that when sodium caseinate-stabilized emulsions are used to encapsulate curcumin, any or all of these tryptophan (hydrophobic) residues directed towards the oil phase could bind to curcumin molecules through hydrophobic interactions and contribute to increasing the loading efficiency of an emulsion (Pan, Zhong, & Baek, 2013). For example, Rao & Khanum (2016) observed a considerable increase in the loading efficiency when the sodium caseinate concentration was increased from 2.5% (89.6%) to 10% (92.3%) in nanoemulsions at a constant curcumin-milk fat ratio of 1:0.05% (w/w) ( Table 2).…”

Section: Loading Efficiencymentioning

confidence: 99%

Recent advances in emulsion-based delivery approaches for curcumin: From encapsulation to bioaccessibility

Araiza‐Calahorra

Akhtar

Sarkar

2018

Trends in Food Science & Technology

355

162

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Background Curcumin has been widely acknowledged for its health-promoting effects. However, its application is often limited by its poor water solubility and biochemical/ structural degradation during physiological transit that restricts its bioavailability. Emulsion based approaches have attracted the most research attention to encapsulate curcumin and improve its stability, bioaccessibility and bioavailability. Scope and approach This review summarizes the recent advances in application of different oil-in-water emulsion-based approaches, such as, conventional emulsions (surfactants-, protein-and protein-polysaccharidestabilized emulsions), nanoemulsions, and Pickering emulsions that have been specifically used to deliver curcumin. Particular emphasis is given to factors affecting curcumin solubility, change in crystalline structure of curcumin upon dispersion and encapsulation efficiency. Changes in the droplet size and emulsion stability during in vitro oral-to-gastrointestinal digestion are discussed, with clear 34 focus on the bioaccessibility of the encapsulated curcumin. 35 36 Key findings and conclusions 37 Key factors that influence curcumin delivery include emulsion droplet size, oil composition, volume fraction, dispersion conditions of curcumin in the oil phase and the type of interfacial materials. Nanoemulsions have been the preferred choice for delivery of curcumin up to now. Although scarce in literature, emulsions stabilized by edible Pickering particles as shown by recent evidence are effective in protecting curcumin in an in vitro gastrointestinal setting due to their high coalescence stability. Further studies with emulsions stabilized by food-grade particles and accurate tracking of the physiological fate (in vitro to human trials) of different emulsion-based delivery vehicles are essential for rational designing of curcumin-rich functional foods with high bioaccessibility.

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“…The encapsulation efficiency can be considered high, since different nanosystems described in the literature (polymer nanoparticle, solid lipid nanoparticle, micelles, liposomes and others) are not able to solubilize and incorporate such amount of PS or hydrophilic molecules [24][25][26].…”

Section: Incorporation Of Ps Into Nlcs and Encapsulation Efficiency Dmentioning

confidence: 99%

Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy

Oshiro

Sato

Boni

et al. 2020

Materials Science and Engineering: C

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Breast cancer is a serious public health problem that causes thousands of deaths annually. Chemotherapy continues to play a central role in the management of breast cancer but is associated with extreme off-target toxicity. Therefore, treatments that directly target the tumor and display reduced susceptibility to resistance could improve the outcome and quality of life for patients suffering from this disease. Photodynamic therapy is a targeted treatment based on the use of light to activate a photosensitizer (PS) that then interacts with molecular oxygen and other biochemical substrates to generate cytotoxic levels of Reactive Oxygen Species. Currently approved PS also tends to have poor aqueous solubility that can cause problems when delivered intravenously. In order to circumvent this limitation, in this manuscript, we evaluate the potential of a phthalocyanine-loaded nanostructured lipid carrier (NLC) functionalized with folic acid (FA). To prepare the FA labelled NLC, the polymer PF127 was first esterified with FA and emulsified with an oil phase containing polyoxyethylene 40 stearate, capric/caprylic acid triglycerides, ethoxylated hydrogenated castor oil 40 and the PS zinc phthalocyanine. The resulting PS loaded FA-NLC had a hydrodynamic diameter of 180 nm and were stable in suspension for > 90 days. Interestingly, the amount of singlet oxygen generated upon light activation for the PS loaded FA-NLC was substantially higher than the free PS, yet at a lower PS concentration. The PS was released from the NLC in a sustained manner with 4.13 ± 0.58% and 27.7 ± 3.16% after 30 min and 7 days, respectively. Finally, cytotoxicity assays showed that NLC in the concentrations of 09.1 μM of PS present non-toxic with > 80 ± 6.8% viable and after 90 s of the light-exposed the results show a statistically significant decrease in cell viability (57 ± 4%). The results obtained allow us to conclude that the functionalized NLC incorporated with PS associated with the PDT technique have characteristics that make them potential candidates for the alternative treatment of breast cancer.

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A green chemistry approach for nanoencapsulation of bioactive compound – Curcumin

Cited by 55 publications

References 37 publications

Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

Recent advances in emulsion-based delivery approaches for curcumin: From encapsulation to bioaccessibility

Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy

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