The method of in situ generation was employed to produce binary silver and copper bimetallic nanocomposites (BNCFs) using lesser emission pollutant biological plant species i.e., Tinospora cordifolia (TC) leaf broth as reducing agent. The generated BNCFs were confirmed by different spectrophotometric studies i.e. Fourier Transform Infrared (FTIR), Scanning Electron Microscope (SEM) along with Energy Dispersive X-ray (EDX), X-ray Diffractometer (XRD), Primary and Secondary thermogravimetric (TG & DTG) analysis and Differential Scanning Calorimetry (DSC). The bio synthesized nanocomposite cotton fabrics (NCFs) were found to be spherical in shape and with an average size of 80nm from SEM analysis. The elements silver and copper were confirmed by observing peaks at 3keV and 1keV, respectively, from EDX spectra. The XRD studies revealed the crystalline nature of BNCFs. TG and DTG analysis explained the catalytic activity of silver and copper with lesser thermal stability. The BNCFs showed good tensile properties, using universal testing machine. The BNCFs also exhibited good antibacterial activity against disease producing G+ve and G-Ve bacteria. The BNCFs mat be considered to make bandage cloths, napkins etc., in the medical field.