A Green Method for Synthesis of Silver-Nanoparticles-Diatomite (AgNPs-D) Composite from Pineapple (<i>Ananas comosus</i>) Leaf Extract

Abstract

This study aims to develop a green method to load silver nanoparticles (AgNPs) into the diatomite (D) pores to produce AgNPs-D composite material. The AgNPs were synthesized by pineapple leaf extract at the temperature of 70 °C for 30 min. The composite formation was characterized by UV-Vis, FTIR, TGA, particle sizes analysis, gravimetric, and color observation. The appearance of surface plasmon bands in 440–460 nm confirms the AgNPs formation. The percentage of the AgNO3 which converted to AgNPs was 99.8%. The smallest particle size of AgNPs was 30 nm, obtained in an AgNO3 concentration of 1 mM with a stirring time of 24 h at 70 °C. The colloidal AgNPs were stable for up to 7 days. The adsorption process of AgNPs was marked by the appearance of –C=O and –C–O– groups peak at 1740 and 1366 cm–1 on the FTIR spectrum. By adsorption and gravimetric technique, as much as 1 wt.% of AgNPs were loaded into D pores. The color of diatomite material changes from white to reddish-brown. The TGA analysis showed that the remaining D and AgNPs-D at 580 °C are 98.22% and 95.74%, respectively. The AgNPs loading through the green technology technique was expected to increase diatomite application in the biomedical field.

Synthesis and Characterization of Proteolytic Enzyme Loaded on Silver Nanoparticles

“…The current study corresponds to the study of [22], where the results of the FTIR analysis of the aqueous solution of pineapple leaves and AgNPs synthesized by pineapple leaves showed that natural substances such as sugars (sucrose, glucose) are responsible for the biosynthetic reduction of Ag + and the formation of AgNPs. This also corresponds to the study of [23], where the results of the FTIR showed the presence of hydroxyl, carbonyl, and carboxyl groups of the main compounds present in pineapple leaf extract, and these groups are responsible for the Ag + reduction and stability of the AgNPs synthesized. Moreover, a study by [24] found that plant-based proteolytic enzymes can be used as agents for the reduction and stability of metal ions for the synthesis of nanoparticles by synthesizing gold nanoparticles using the bromelain enzyme.…”