A Green Three-Ratio Manipulating Spectrophotometric ApproachesFor the determination of a binary mixture of pantoprazole and domperidone

Abstract

Pantoprazole (PAN), a proton pump inhibitor, is widely prescribed to treat gastrointestinal disorders such as gastroesophageal reflux disorder (GERD), and hyperacidity. Suppressing gastric acid secretion effectively alleviates symptoms like heartburn, acid regurgitation, and indigestion. Domperidone (DOM), a prokinetic agent, that enhances gastrointestinal motility is often prescribed with PAN in patients with additional motility disorders. This combination is useful for patients who suffer from nausea, vomiting, or bloating due to motility dysfunction. (Pantosec-D) tablets contain both PAN and DOM This combination provides rapid relief from gastrointestinal symptoms by addressing both acid-related and motility-related issues. This combination ensures better management of gastrointestinal problems by enhancing the patient's comfort and quality of life. The establishment and validation of three spectrophotometric techniques—the ratio difference (RD), the first derivatives (1DD), and the mean centering (MC) methods of the ratio spectra—for determining PAN and DOM in combined pharmaceutical formulations are presented in this study. These methods are based on manipulating the ratio spectra of the cited medications to resolve their spectral overlapping, including the RD, 1DD, and MC methods. DOM was measured using the RD method, which calculated the amplitude difference between 209 and 233 nm. Conversely, PAN was evaluated by determining the 254 and 223 nm amplitude differences. DOM and PAN were shown to be selectively determined at 215 nm and 249 nm, respectively, using the first derivatives of the ratio spectra approach. PAN was evaluated at 254 nm in the mean centering method, whereas DOM was specifically quantified at 209 nm. The suggested procedures were verified in compliance with ICH regulations. Pharmaceutical formulations comprising PAN and DOM were effectively analyzed using the linear correlations obtained for both drugs over concentration ranges of zero–52 µg/mL and 1–18 µg/mL, respectively. Compared with reported chromatographic methods, these techniques provide easy-to-use, reasonably priced, and dependable substitutes for the standard quality control of these medications in mixed dosage forms.