2022
DOI: 10.1101/2022.08.01.502415
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A greenhouse-based high-throughput phenotyping platform for identification and genetic dissection of resistance to Aphanomyces root rot in field pea

Abstract: Aphanomyces root rot (ARR) is a devastating disease in field pea (Pisum sativum L.) that can cause up to 100% crop failure. Assessment of ARR resistance can be a rigorous, costly, time-demanding activity that is relatively low-throughput and prone to human errors. These limits the ability to effectively and efficiently phenotype the disease symptoms arising from ARR infection, which remains a perennial bottleneck to the successful evaluation and incorporation of disease resistance into new cultivars. In this s… Show more

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Cited by 1 publication
(2 citation statements)
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“…Subsequently, the DNA samples obtained were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR). As described by Bari et al (2022), DNA samples were standardized to a final concentration of 25 ng/µl for subsequent genotyping-by-sequencing (GBS) at a genomic center. The prepared dual-indexed GBS libraries using the restriction enzyme ApeKI (Elshire et al 2011) were combined into a single pool and sequenced across 1.5 lanes of NovaSeq S1x100-pb run, producing approximately 1,000 million pass filter reads with mean quality scores of > 30.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Subsequently, the DNA samples obtained were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR). As described by Bari et al (2022), DNA samples were standardized to a final concentration of 25 ng/µl for subsequent genotyping-by-sequencing (GBS) at a genomic center. The prepared dual-indexed GBS libraries using the restriction enzyme ApeKI (Elshire et al 2011) were combined into a single pool and sequenced across 1.5 lanes of NovaSeq S1x100-pb run, producing approximately 1,000 million pass filter reads with mean quality scores of > 30.…”
Section: Methodsmentioning
confidence: 99%
“…The genetic materials consisted of 282 NDSU advanced elite breeding lines previously described in Bari et al (2022). The lines were planted in 1.5-x 7.6-m plots at 0.30-m spacing between plots with 840 pure live seeds per plot, arranged in an augmented incomplete block design with five diagonal repeated checks for preliminary yield trials.…”
Section: Germplasm and Phenotypingmentioning
confidence: 99%