2021
DOI: 10.3389/fcell.2021.698254
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A GSK3-SRF Axis Mediates Angiotensin II Induced Endothelin Transcription in Vascular Endothelial Cells

Abstract: Endothelin, encoded by ET1, is a vasoactive substance primarily synthesized in vascular endothelial cells (VECs). Elevation of endothelin levels, due to transcriptional hyperactivation, has been observed in a host of cardiovascular diseases. We have previously shown that serum response factor (SRF) is a regulator of ET1 transcription in VECs. Here we report that angiotensin II (Ang II) induced ET1 transcription paralleled activation of glycogen synthase kinase 3 (GSK3) in cultured VECs. GSK3 knockdown or pharm… Show more

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Cited by 8 publications
(6 citation statements)
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“…25 Its dephosphorylation and activation by C-reactive protein was reported to promote EC activation, 52 and it mediated angiotensin II-induced endothelin expression in vascular ECs. 53 AMPK is a stress-responsive protein that protects against cardiomyocyte injury during IRI, mainly through maintaining mitochondrial function. 54 Pharmacological activation of AMPK was reported to activate eNOS (endothelial nitric oxide synthase) and inhibit NFκB, thereby ameliorating endothelial dysfunction and inflammation.…”
Section: Discussionmentioning
confidence: 99%
“…25 Its dephosphorylation and activation by C-reactive protein was reported to promote EC activation, 52 and it mediated angiotensin II-induced endothelin expression in vascular ECs. 53 AMPK is a stress-responsive protein that protects against cardiomyocyte injury during IRI, mainly through maintaining mitochondrial function. 54 Pharmacological activation of AMPK was reported to activate eNOS (endothelial nitric oxide synthase) and inhibit NFκB, thereby ameliorating endothelial dysfunction and inflammation.…”
Section: Discussionmentioning
confidence: 99%
“…Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (50 mM Tris pH7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche) as previously described [ 37 ]. Nuclear proteins were prepared with the NE-PER Kit (Pierce) following manufacturer's recommendation.…”
Section: Methodsmentioning
confidence: 99%
“…RNA was extracted with the RNeasy RNA isolation kit (Qiagen). Reverse transcriptase reactions were performed using a SuperScript First-strand Synthesis System (Invitrogen) as previously described ( Dong et al, 2020 ; Hong et al, 2020 ; Wu T. et al, 2020 ; Yang et al, 2020a , b , 2021 ; Zhang et al, 2020 ; Kong et al, 2021b ). Real-time PCR reactions were performed on an ABI Prism 7500 system.…”
Section: Methodsmentioning
confidence: 99%