A guideline proposal for mice preparation and care in 18F-FDG PET imaging

Abstract

The experimental outcomes of small-animal positron emission tomography (PET) imaging with 18F-labelled fluorodeoxyglucose (18F-FDG) can be particularly compromised by animal preparation and care. Several works intend to improve research reporting and amplify the quality and reliability of published research. Though these works provide valuable information to plan and conduct animal studies, manuscripts describe different methodologies—standardization does not exist. Consequently, the variation in details reported can explain the difference in the experimental results found in the literature. Additionally, the resources and guidelines defining protocols for small-animal imaging are scarce, making it difficult for researchers to obtain and compare accurate and reproducible data. Considering the selection of suitable procedures key to ensure animal welfare and research improvement, this paper aims to prepare the way for a future guideline on mice preparation and care for PET imaging with 18F-FDG. For this purpose, a global standard protocol was created based on recommendations and good practices described in relevant literature.

Optimisation of animal handing and timing of 2-deoxy-2-[18F]fluoro-D-glucose PET tumour imaging in mice

“…In mouse imaging there have been recent efforts to standardise image acquisition [14,17]. Tumour uptake typically shows a period of rapid uptake before a plateau.…”

Dissociable roles of the mPFC-to-VTA Pathway in the control of iImpulsive Action and Risk-Related Decision-Making in Roman High- And Low-Avoidance Rats

“…Scans were performed one week apart and the treatments were administered in a counterbalanced order. Animals were fasted for 12 hours before each scan to minimize competition between dietary glucose and the [ 18 F]-FDG radiotracer (Ribeiro et al, 2022). Rats then received an ip.…”

“…The doses injected were not significantly different between rat lines or Saline/CNO conditions (RHA: SAL=39.07±4.52 MBq and CNO=38.34±6.38 MBq; RLA SAL=41.08±4.65 MBq and CNO=40.50±4.11 MBq; Line: F (1,33) =1.70, p=0.20; Time: F (1,33) =1.36, p=0.25; Line × Time: F (1,33) =0.018, p=0.89). After [ 18 F]-FDG injection, rats were placed in standard home cages and kept warm with a red-light lamp to prevent brown tissue uptake of the radiotracer (Ribeiro et al, 2022). At 45min uptake, anesthesia was initiated and maintained with isoflurane (2.5%) in oxygen, and the rats were positioned by two in the micro-PET scanner Triumph II (TriFoil Imaging, Northridge, CA, USA) using a compatible custom-made stereotaxic-like frame.…”

Engineering Nanoplatforms for Theranostics of Atherosclerotic Plaques

“…To solve the problems, nanomaterial-based PET imaging tracers have been recently employed to identify plaques in the early stages of AS, thereby enhancing the diagnostic accuracy of atherosclerotic plaques. [126,127] Examples of typical nanomaterial-based PET imaging tracers encompass the isotope flourine-18 ( 18 F) and zirconium-89 ( 89 Zr). [128] For instance, Nahrendorf et al developed functionalized 18 F-labeled polyglucose nanoparticles called Macroflor, which exhibited rapid renal excretion and could serve as PET imaging CAs for precise and quantitative detection of atherosclerotic plaques (Figure 4A).…”

“…To solve the problems, nanomaterial‐based PET imaging tracers have been recently employed to identify plaques in the early stages of AS, thereby enhancing the diagnostic accuracy of atherosclerotic plaques. [ 126,127 ] Examples of typical nanomaterial‐based PET imaging tracers encompass the isotope flourine‐18 ( 18 F) and zirconium‐89 ( 89 Zr). [ 128 ] For instance, Nahrendorf et al.…”