A gut-secreted peptide suppresses arousability from sleep

Titos, Iris; Juginović, Alen; Vaccaro, Alexandra; Nambara, Keishi; Gorelik, Pavel; Mazor, Ofer; Rogulja, Dragana

doi:10.1016/j.cell.2023.02.022

Cited by 32 publications

(7 citation statements)

References 123 publications

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“…Moreover, some investigations have revealed bidirectional communication between the peripheral system and the brain via neural networks, which play important roles in regulating sleep and emotion. , Therefore, to explore the vital targets of CP in alleviating CUMS-induced anxiety and insomnia and to determine whether this amelioration passed through the association between the peripheral system and the brain HPA response or inflammation, PCA was performed, and the Pearson correlation coefficient (PCC) was determined for the first time. The results showed that principal component 1 (PC 1) explained 48.6% of the variability in the data set, and PC 2 was responsible for 34.6% of the data set in the brain (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Comparison of the Protective Effects of Casein Hydrolysate Containing Tyr-Pro-Val-Glu-Pro-Phe and Casein on the Behaviors and Peripheral and Brain Functions in Mice with Chronic-Stress-Induced Anxiety and Insomnia

Qian,

Yu,

Zheng

et al. 2024

J. Agric. Food Chem.

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Chronic stress is a major inducer of anxiety and insomnia. Milk casein has been studied for its stress-relieving effects. We previously prepared a casein hydrolysate (CP) rich in the sleep-enhancing peptide YPVEPF, and this study aims to systemically investigate the different protective effects of CP and casein on dysfunction and anxiety/insomnia behavior and its underlying mechanisms in chronically stressed mice. Behavioral results showed that CP ameliorated stress-induced insomnia and anxiety more effectively than milk casein, and this difference in amelioration was highly correlated with an increase in GABA, 5-HT, GABA A , 5-HT 1A receptors, and BDNF and a decrease in IL-6 and NMDA receptors in stressed mice. Furthermore, CP restored these dysfunctions in the brain and colon by activating the HPA response, modulating the ERK/CREB-BDNF-TrκB signaling pathway, and alleviating inflammation. The abundant YPVEPF (1.20 ± 0.04%) and Tyr-based/Trp-containing peptides of CP may be the key reasons for its different effects compared to casein. Thus, this work revealed the main active structures of CP and provided a novel dietary intervention strategy for the prevention and treatment of chronic-stress-induced dysfunction and anxiety/insomnia behaviors.

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Section: Resultsmentioning

confidence: 99%

Comparison of the Protective Effects of Casein Hydrolysate Containing Tyr-Pro-Val-Glu-Pro-Phe and Casein on the Behaviors and Peripheral and Brain Functions in Mice with Chronic-Stress-Induced Anxiety and Insomnia

Qian,

Yu,

Zheng

et al. 2024

J. Agric. Food Chem.

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“…Neuropeptides in insect guts take part in the regulation of feeding processes including food choice, appetite, digestion, metabolism, and excretion (Abou El Asrar et al, 2020). DH31, CCHa‐1, CCHa‐2, and NPF are well‐known neuropeptides associated with the gut‐brain axis, regulating appetite, sleep, and even courtship (Lin et al, 2022; Malita et al, 2022; Sano et al, 2015; Titos et al, 2023). As listed in flyAtlas expression database, a few other neuropeptide signal genes were also expressed in Drosophila guts, including AstC, Bura, ILP3, ITP, MS, Limostatin, NPLP2 and TK, and receptors for AstA, CAPA, CCHa‐1, DHs, GPA2/GPB5, ILPs, LK, NPF, NPLP1, RYa, and TK (Nässel and Zandawala, 2019).…”

Section: Discussionmentioning

confidence: 99%

Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Huang,

Dong,

Yang

et al. 2024

Arch Insect Biochem Physiol

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The predatory stink bug Arma custos has been selected as an effective biological control agent and has been successfully massly bred and released into fields for the control of a diverse insect pests. As a zoophytophagous generalist, A. custos relies on a complex neuropeptide signaling system to prey on distinct food and adapt to different environments. However, information about neuropeptide signaling genes in A. custos has not been reported to date. In the present study, a total of 57 neuropeptide precursor transcripts and 41 potential neuropeptide G protein‐coupled receptor (GPCR) transcripts were found mainly using our sequenced transcriptome data. Furthermore, a number of neuropeptides and their GPCR receptors that were enriched in guts and salivary glands of A. custos were identified, which might play critical roles in feeding and digestion. Our study provides basic information for an in‐depth understanding of biological and ecological characteristics of the predatory bug and would aid in the development of better pest management strategies based on the effective utilization and protection of beneficial natural enemies.

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“…Piezo potentially influences sleep latency through satiety. Recent work demonstrates that dietary protein promotes sleep through peptidergic signaling in the posterior gut, specifically inhibiting the response to mechanical stimulation 59 . It would be interesting to determine whether Piezo , which functions to sense fullness in the anterior gut 60 , has a role in this signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency

Eiman,

Kumar,

Serrano Negron

et al. 2024

Sci Rep

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Sleep latency, the amount of time that it takes an individual to fall asleep, is a key indicator of sleep need. Sleep latency varies considerably both among and within species and is heritable, but lacks a comprehensive description of its underlying genetic network. Here we conduct a genome-wide association study of sleep latency. Using previously collected sleep and activity data on a wild-derived population of flies, we calculate sleep latency, confirming significant, heritable genetic variation for this complex trait. We identify 520 polymorphisms in 248 genes contributing to variability in sleep latency. Tests of mutations in 23 candidate genes and additional putative pan-neuronal knockdown of 9 of them implicated CG44153, Piezo, Proc-R and Rbp6 in sleep latency. Two large-effect mutations in the genes Proc-R and Piezo were further confirmed via genetic rescue. This work greatly enhances our understanding of the genetic factors that influence variation in sleep latency.

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A gut-secreted peptide suppresses arousability from sleep

Cited by 32 publications

References 123 publications

Comparison of the Protective Effects of Casein Hydrolysate Containing Tyr-Pro-Val-Glu-Pro-Phe and Casein on the Behaviors and Peripheral and Brain Functions in Mice with Chronic-Stress-Induced Anxiety and Insomnia

Comparison of the Protective Effects of Casein Hydrolysate Containing Tyr-Pro-Val-Glu-Pro-Phe and Casein on the Behaviors and Peripheral and Brain Functions in Mice with Chronic-Stress-Induced Anxiety and Insomnia

Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae)

Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency

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