A H₂S-Triggered Dual-Modal Second Near-Infrared/Photoacoustic Intelligent Nanoprobe for Highly Specific Imaging of Colorectal Cancer

Abstract

An endogenous H 2 S-triggered intelligent optical nanoprobe combining second near-infrared (NIR-II) fluorescence with photoacoustic (PA) imaging can provide more comprehensive information to further improve the sensitivity and reliability of diagnosis for colorectal tumor, which is rarely explored. Herein, an endogenous H 2 S-triggered SiO 2 @Ag nanoprobe was designed for in situ dual-modal NIR-II/PA imaging of colorectal cancer. The designed dual-modal nanoprobe can be converted to SiO 2 @Ag 2 S after in situ biosynthesis via a sulfuration reaction with the overexpressed endogenous H 2 S in the colorectal tumor. More importantly, the designed SiO 2 @Ag nanoprobe exhibits high sensitivity and specificity for diagnosing colorectal cancer in vivo via dual-modal NIR-II/PA imaging. These results provide a new NIR-II/PA dual-modal imaging strategy for noninvasive intelligent detection of colorectal cancer.

Near-Infrared II Gold Nanocluster Assemblies with Improved Luminescence and Biofate for In Vivo Ratiometric Imaging of H2S

“…NIR-II imaging results also revealed the ratiometric PL imaging of H 2 S (Figure e). As summarized in Table S1, the detection sensitivity of this nanoprobe is comparable to that of the reported AuNC-based systems but slightly lower than that of some imaging bioprobes. − It is worth mentioning that our nanoprobe utilizes 808 nm-excited ratiometric NIR-II PL for H 2 S imaging, which could not only improve the in vivo tissue penetration depth and imaging contrast but also bypass possible thermal injury caused by 980-nm laser irradiation. Significantly, the H 2 S detection sensitivity of the developed imaging platforms might be further improved by increasing the luminescence efficacy of NIR-II-emitting AuNCs (through metal ion doping, ligand engineering strategies, etc.…”

Enhanced immunotherapy based on the synergistic click reaction-mediated chemotherapy and photothermal therapy for efficient tumor inhibition

“…The temperature of TPE-Ni/OPYO NPs solution was significantly increased to 64 o C within 10 min under the irradiation of 940 nm laser (1.0 W cm −2 ) (Figure 2f). The photothermal conversion efficiency (PCE, η) was calculated to be 28.9% (Figure S10), which was much higher than that of ICG (17.3% 45 ) and Au nanorods (21% 46 ). In addition, the photothermal effect shows a laser irradiation power dependence and TPE-Ni concentration dependence, indicating that the heat generation could be well controlled (Figure S11).…”

Implications of hydrogen sulfide in colorectal cancer: Mechanistic insights and diagnostic and therapeutic strategies

“…The designed amphiphilic probes 1 and 2 are able to spontaneously self-assemble into nanoprobes with well-defined nanostructures and show an unprecedented aggregation-enhanced responsiveness to H 2 S. This probe in the aggregated state rather than the molecularly soluble state emits NIR fluorescence as well as PA signals upon specific activation by H 2 S, thus allowing in vivo visualization and differentiation of CRC based on differences in H 2 S content. In addition, Bi and co-workers developed a NIR-II/PA dual-mode nanoprobe (SiO 2 @Ag) based on the H 2 S-mediated in situ sulfation reaction for specific monitoring of colorectal tumors [ 302 ]. Upon activation by endogenous H 2 S, this nanoprobe present turned-on NIR-II fluorescence from 1000 to 1400 nm as well as an excellent PA signal, showing high sensitivity and specificity in the diagnosis of colon cancer in vivo.…”