A H2O2-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis

Leuthner, Birgitta; Aichinger, Christian; Oehmen, E.; Koopmann, Edda; Müller, Olaf; Müller, Philip; Kahmann, Regine; Bölker, Michael; Schreier, Peter

doi:10.1007/s00438-004-1085-6

Cited by 87 publications

(80 citation statements)

References 51 publications

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“…BLASTP of NCBI shows CRO1 most closely related to conceptual translations from the basidiomycetes Cryptococcus neoformans genomes (e.g., gi57227801, score 607) and Ustilago maydis glo2 (gi33386644, score 519). The latter gene belongs to a family of glx-like genes recently studied by Leuthner et al (29). cro1 is located on scaffold 6 (coordinates 592442 to 594775), distantly linked to glx (coordinates 1201617 to 1203348).…”

Section: Resultsmentioning

confidence: 99%

“…Ustilago maydis glo1 (gi71013128, score 522) is the most closely related sequence in the current NCBI database. The membrane-bound Glo1 protein is involved in filamentous growth and pathogenicity of U. maydis (29). A variant cDNA clone extending the coding region by 102 amino acids also was amplified by reverse transcription-PCR (RT-PCR) from P. chrysosporium CC41 cultures and from colonized wood (GenBank DQ400693).…”

Section: Resultsmentioning

confidence: 99%

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Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Wymelenberg

Sabat

Mozuch

et al. 2006

Appl Environ Microbiol

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The white rot basidiomycete Phanerochaete chrysosporium produces an array of nonspecific extracellular enzymes thought to be involved in lignin degradation, including lignin peroxidases, manganese peroxidases, and the H 2 O 2 -generating copper radical oxidase, glyoxal oxidase (GLX). Preliminary analysis of the P. chrysosporium draft genome had identified six sequences with significant similarity to GLX and designated cro1 through cro6. The predicted mature protein sequences diverge substantially from one another, but the residues coordinating copper and constituting the radical redox site are conserved. Transcript profiles, microscopic examination, and lignin analysis of inoculated thin wood sections are consistent with differential regulation as decay advances. The cro2-encoded protein was detected by liquid chromatography-tandem mass spectrometry in defined medium. The cro2 cDNA was successfully expressed in Aspergillus nidulans under the control of the A. niger glucoamylase promoter and secretion signal. The recombinant CRO2 protein had a substantially different substrate preference than GLX. The role of structurally and functionally diverse cro genes in lignocellulose degradation remains to be established.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Wymelenberg

Sabat

Mozuch

et al. 2006

Appl Environ Microbiol

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“…They are highly variable, but some functional classes have been identified. These include serine and cysteine protease inhibitors that target host proteases (1248,1249), effectors that minimize the levels of ROS (1250,1251), proteins that protect fungal cell walls against hydrolysis by plant chitinases (1252), and phytotoxic proteins. Apoplastic effectors may be involved in masking fungal detection by the plant by operating as protectors of fungal proteins (1253,1254).…”

Section: Common Features Of Known Effector Proteinsmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

165

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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“…The hydrogen peroxide appears to be involved in the degradation of lignin, because ligninase produced by the fungus needs this compound for its enzymatic activity [Leuthner et al, 2005].…”

Section: Characterization and Expression Analyses Of The Selected Cdnmentioning

confidence: 99%

Morphological and Molecular Modifications Induced by Different Carbohydrate Sources in <i>Tuber borchii</i>

Amicucci

Zambonelli²,

Iotti³

et al. 2010

Microb Physiol

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During the life cycle of mycorrhizal fungi, morphological, genetic and metabolic modifications are induced in the fungus and its symbiotic partner. These changes are influenced by environmental factors: light, gravity, oxygen, temperature, soil type, nutrients, root exudates and the presence of particular bacterial and perhaps fungal and viral populations in the mycorrhizosphere. To determine whether different carbohydrates lead to cell-signalling events and morphofunctional changes in cultured Tuber borchii mycelia, the expression level of genes involved in morphological modifications was investigated using a macroarray technique and real-time RT-PCR. The morphological study showed an increased growth of Tuber mycelia in glucose, while the hyphae were thinner and less branched in sucrose and maltose. This was accompanied by an upregulation of the genes involved in the general cell metabolism, detoxification processes, hyphal growth and cytoskeleton organization. Since glucose is also present in root exudates, the increased expression of these genes might support the hypothesis that glucose can act as a signal for the fungus to indicate the presence of the plant, and to trigger the complex symbiotic process. These mechanisms can lead to morphological modifications, including increased branching of the root which is necessary for the fungus to establish the symbiosis.

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A H2O2-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis

Cited by 87 publications

References 51 publications

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Morphological and Molecular Modifications Induced by Different Carbohydrate Sources in <i>Tuber borchii</i>

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