A haemocytolytic assay for tiger prawn,Penaeus monodon

Screening of gastrointestinal bacteria of Indian major carps for a candidate probiotic species for aquaculture practices

“…Although, a correlation between haemolysis and the pathogenic nature of bacteria has not been established (Inamura, Muroga & Nakai 1984; Kodama, Moustofa, Mikami & Izawa 1985), harmful haemolytic bacteria can initiate infection by entering small lesions in the skin and mucous membranes in any host (Madigan, Martinko & Parker 1984) and is commonly used as an indicator for differentiation of pathogens (Ullah & Arai 1983; Chen, Lai & Huang 1995; Lee, Chen & Liu 1995). In the present investigation, GI isolates belong to Plesiomonas , Pseudomonas , Aeromonas species including A. hydrophila were haemolytic in nature.…”

Isolation of a pathogenic strain of Vibrio alginolyticus from necrotic larvae of Macrobrachium rosenbergii (de Man)

“…Zhang & Austin (2000) highlighted the importance of haemolysin as a virulence factor of pathogenic vibrios in fish. Additionally, the haemolytic assay has been recognized to be useful in differentiating the virulent strains among suspected pathogens by Ullah & Arai (1983), Chen, Lai & Huang (1995) and Lee, Chen & Liu (1995). Chang et al (2000) used prawn blood agar haemolysis to screen bacteria pathogenic to cultured tiger prawn, Penaeus monodon.…”

Use of prawn blood agar hemolysis to screen for bacteria pathogenic to cultured tiger prawns Penaeus monodon

“…However, both pathogenic and non-pathogenic bacteria are often found in the prawn hepatopancreas (Ruangpan & Kitao 1991, Chang et al 1996a) and it has been suggested that their hemolytic activity may be correlated to their pathogenicity, since factors such as adhesion and invasion (Ruangpan et al 1994) have been excluded. Although there is still much disagreement about the relationship between hemolysis and pathogenicity (Inamura et al 1984, Chung & Kou 1985, Kodama et al 1985, hemocytolytic assay is still commonly used to differentiate among suspected pathogens (Ullah & Arai 1983, Lee et al 1995.…”

“…Conventional methods of hemocytolytic assay are carried out either using commercial sheep blood agar (Collins & Lyne 1984) or by direct observation of hemocytes under a microscope (Lee et al 1995). The latter method requires staining of the hemocytes followed by continuous observation and is not suitable for efficient screening of large numbers of bacterial isolates from tiger prawns and their culture environment because it is labor-intensive and time-consuming.…”