A Haemolytic V-Dependent Co2-Preferring Haemophilus Species (HaemophilUs Paraphro-Haemolyticus Nov. Spec.)

Zinnemann, K.; Rogers, Kandace; Frazer, Joyce; Devaraj, S.K.

doi:10.1099/00222615-4-1-139

Cited by 16 publications

(6 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional tests were performed according to the techniques described by Zinnemann et al (1968Zinnemann et al ( , 1971 for characterisation of the species described by these authors. The Haemophilus strains were assigned to species according to the taxonomy proposed by Zinnemann and Biberstein (1974).…”

Section: Methods Of Examinationmentioning

confidence: 99%

“…Identification of the strains was based on morphology, requirement for growth factors (using BBL disks containing either or both of the two X and V growth factors), and haemolytic activity examined after growth on 5 % defibrinated horse blood in Heart Infusion Agar (Difco) supplemented with 5 % Fildes enrichment (Difco). Additional tests were performed according to the techniques described by Zinnemann et al (1968Zinnemann et al ( , 1971 for characterisation of the species described by these authors. The Haemophilus strains were assigned to species according to the taxonomy proposed by Zinnemann and Biberstein (1974).…”

Section: Organismsmentioning

confidence: 99%

See 1 more Smart Citation

Significance of the detection of beta-galactosidase and of beta-xylosidase in the taxonomic study of the genus Haemophilus.

Hansen¹,

Schoutens²,

Yourassowsky³

1979

J Clin Pathol

View full text Add to dashboard Cite

SUMMARY A total of 314 strains of Haemophilus, isolated from clinical samples, were studied for the production of beta-galactosidase and beta-xylosidase. None of the H. influenza strains studied (9 beta-lactamase positive strains and 129 beta-lactamase negative strains) possessed these enzymes. Both enzymes were almost constantly observed among strains of H. paraphrophilus (10 strains studied) and of H. paraphrohaemolyticus (9 strains studied). Among the other species (H. parainfluenzae, 55 strains; H. haemolyticus, 5 strains; H. parahaemolyticus, 97 strains), beta-galactosidase was present in about 30 % of the strains studied whereas beta-xylosidase was detected occasionally (3 % of the strains studied). Detection of these two enzymes could be a valuable test for the taxonomic study of the genus Haemophilus. However, the type of substrate used for the detection of betaxylosidase is important: use of the para-nitro-phenyl-beta-xylopyranoside yielded more positive results than the use of its ortho-isomer.

show abstract

Section: Methods Of Examinationmentioning

confidence: 99%

Section: Organismsmentioning

confidence: 99%

Significance of the detection of beta-galactosidase and of beta-xylosidase in the taxonomic study of the genus Haemophilus.

Hansen¹,

Schoutens²,

Yourassowsky³

1979

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…paraphrohaemolyticus was originally described as a species distinct from H. parahaemolyticus due to its CO 2 -stimulated growth (102), but this phenotypic trait is unreliable; indeed, one of the three original strains deposited by Zinnemann and coworkers was later shown to belong to H. parainfluenzae (99). Instead, H. parahaemolyticus has been separated from H. paraphrohaemolyticus by the former's ability to produce IgA1 protease and the latter's ability to produce ␤-galactosidase (48).…”

Section: The Haemophilus Parainfluenzae Groupmentioning

confidence: 99%

Classification, Identification, and Clinical Significance of Haemophilus and Aggregatibacter Species with Host Specificity for Humans

2014

View full text Add to dashboard Cite

SUMMARY The aim of this review is to provide a comprehensive update on the current classification and identification of Haemophilus and Aggregatibacter species with exclusive or predominant host specificity for humans. Haemophilus influenzae and some of the other Haemophilus species are commonly encountered in the clinical microbiology laboratory and demonstrate a wide range of pathogenicity, from life-threatening invasive disease to respiratory infections to a nonpathogenic, commensal lifestyle. New species of Haemophilus have been described ( Haemophilus pittmaniae and Haemophilus sputorum ), and the new genus Aggregatibacter was created to accommodate some former Haemophilus and Actinobacillus species ( Aggregatibacter aphrophilus , Aggregatibacter segnis , and Aggregatibacter actinomycetemcomitans ). Aggregatibacter species are now a dominant etiology of infective endocarditis caused by fastidious organisms (HACEK endocarditis), and A. aphrophilus has emerged as an important cause of brain abscesses. Correct identification of Haemophilus and Aggregatibacter species based on phenotypic characterization can be challenging. It has become clear that 15 to 20% of presumptive H. influenzae isolates from the respiratory tracts of healthy individuals do not belong to this species but represent nonhemolytic variants of Haemophilus haemolyticus . Due to the limited pathogenicity of H. haemolyticus , the proportion of misidentified strains may be lower in clinical samples, but even among invasive strains, a misidentification rate of 0.5 to 2% can be found. Several methods have been investigated for differentiation of H. influenzae from its less pathogenic relatives, but a simple method for reliable discrimination is not available. With the implementation of identification by matrix-assisted laser desorption ionization–time of flight mass spectrometry, the more rarely encountered species of Haemophilus and Aggregatibacter will increasingly be identified in clinical microbiology practice. However, identification of some strains will still be problematic, necessitating DNA sequencing of multiple housekeeping gene fragments or full-length 16S rRNA genes.

show abstract

“…The rare occurrence of this species in clinical samples is underscored by the mistaken identity of strain NCTC 10672 (=ATCC 29239), one of the original 3 strains (strain Corinthian) deposited as H. paraphrohaemolyticus by Zinnemann et al (1971). According to the present study, strain NCTC 10672 belongs to H. parainfluenzae.…”

Section: Discussionmentioning

confidence: 59%

“…She found the species to be frequently associated with acute pharyngitis and occasionally with subacute endocarditis (Pittman, 1953). H. paraphrohaemolyticus was later described as a species distinct from H. parahaemolyticus due to its CO 2 -stimulated growth (Zinnemann et al, 1971). Kilian (1976) was unable to validate H. parahaemolyticus as a separate species by extended phenotypic testing, because only the presence of haemolysis discriminated this species from H. parainfluenzae biotype III, and haemolysis is a variable trait of H. parainfluenzae (Kilian, 1976).…”

Section: Introductionmentioning

confidence: 99%

Identification of haemolytic Haemophilus species isolated from human clinical specimens and description of Haemophilus sputorum sp. nov

Nørskov‐Lauritsen

Bruun

Andersen

et al. 2012

International Journal of Medical Microbiology

View full text Add to dashboard Cite

A Haemolytic V-Dependent Co2-Preferring Haemophilus Species (HaemophilUs Paraphro-Haemolyticus Nov. Spec.)

Cited by 16 publications

References 1 publication

Significance of the detection of beta-galactosidase and of beta-xylosidase in the taxonomic study of the genus Haemophilus.

Significance of the detection of beta-galactosidase and of beta-xylosidase in the taxonomic study of the genus Haemophilus.

Classification, Identification, and Clinical Significance of Haemophilus and Aggregatibacter Species with Host Specificity for Humans

Identification of haemolytic Haemophilus species isolated from human clinical specimens and description of Haemophilus sputorum sp. nov

Contact Info

Product

Resources

About