A hand-held fluorescent sensor platform for selectively estimating green algae and cyanobacteria biomass

Shin, Young-Ho; Barnett, Jonathan Z.; Gutierrez-Wing, Maria Teresa; Rusch, Kelly A.; Choi, Jin‐Woo

doi:10.1016/j.snb.2018.02.045

Cited by 29 publications

(29 citation statements)

References 33 publications

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“…This proves that the developed fluorometer can be used in detecting and differentiating the chlorophyll a and phycocyanin pigments simultaneously. The data were used to selectively estimate two different species (green and blue-green algae) using the developed algorithm in our previous work [7]. The lower limit of detection for both chlorophyll a and phycocyanin was set to 1 µg l −1 with 1 µg l −1 resolution.…”

Section: Characterization Of Chlorophyll a And Phycocyanin Fluorescencementioning

confidence: 99%

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A field-deployable and handheld fluorometer for environmental water quality monitoring

Shin

Gutierrez-Wing

Choi

2018

Micro and Nano Syst Lett

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This work reports the development of a field-deployable and fully handheld fluorometer for environmental water monitoring. Our developed fluorimeter can detect both green algae and cyanobacteria (blue-green algae) while simultaneously differentiating and measuring two different species by selectively measuring chlorophyll a fluorescence from green algae and phycocyanin fluorescence from blue-green algae. As a demonstration, chlorophyll a and phycocyanin photopigments were estimated and differentiated. The system was also tested with an environmental water sample collected from a lake to validate the system functionality. The presented results suggest that our developed fluorimeter could be used as a portable water quality monitoring system for detection of different photopigment of algae.

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Section: Characterization Of Chlorophyll a And Phycocyanin Fluorescencementioning

confidence: 99%

“…Previously, we have reported a fluorometer system in [6,7] based on controlled samples. In this work, we demonstrate the functionality of our system at a local lake using environmental water samples collected on site.…”

Section: Introductionmentioning

confidence: 99%

A field-deployable and handheld fluorometer for environmental water quality monitoring

Shin

Gutierrez-Wing

Choi

2018

Micro and Nano Syst Lett

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“…different spectral regions [19,20]. Additionally, fluorescence properties of intracellular molecules and structures can be used as readout, leading to different autofluorescence spectra in organisms [21][22][23][24]. Furthermore, the difference within structural composition enables discrimination of cell types and strains by staining technique with specific dyes to create contrast between different species.…”

Section: Open Accessmentioning

confidence: 99%

Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes

Schlembach

Grünberger

Regestein

2021

Trends in Biotechnology

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Microbial mixed cultures are gaining increasing attention as biotechnological production systems, since they offer a large but untapped potential for future bioprocesses. Effects of secondary metabolite induction and advantages of labor division for the degradation of complex substrates offer new possibilities for process intensification. However, mixed cultures are highly complex, and, consequently, many biotic and abiotic parameters are required to be identified, characterized, and ideally controlled to establish a stable bioprocess. In this review, we discuss the advantages and disadvantages of existing measurement techniques for identifying, characterizing, monitoring, and controlling mixed cultures and highlight promising examples. Moreover, existing challenges and emerging technologies are discussed, which lay the foundation for novel analytical workflows to monitor mixed-culture bioprocesses. Mixed Cultures: Opportunities and Challenges An old tradition is revolutionizing modern biotechnology: microbial collaboration. Mixed-culture applications, such as wastewater treatment, composting, and a broad spectrum of fermentative food preparations, are the historical foundation of current biotechnology. Now, a targeted assembly of microorganisms to perform concerted bioproductions is forming a new cutting edge in biotechnology. Over the past decade, the research field of defined mixed cultures has gained increased attention due to their potential for process intensification and the chance to produce unknown secondary metabolites [1]. In the old-school mixed-culture approach, the driving force to develop mixed culture-specific (online) measurement techniques was very limited. Reasons for this might be the rather low dynamic of these processes and the strong closeness to natural processes and equilibria with no need for control. In other words, there was simply no need for a deeper understanding of these robust systems. However, this is now changing. Since sustainability is currently the most important driving force in biotechnology, the number of published studies on mixed-culture consolidated bioprocesses (see Glossary) with plant biomass, waste streams, and gaseous waste substrates is increasing significantly [2-9]. The products of these processes are, in many cases, acids, alcohols, or enzymes for biomass pretreatment and hydrolysis [6,9]. Higher-value products of mixed cultures are secondary metabolites induced by interspecies communication [1,10-14]. Among these, antimicrobial substances, such as antibiotics, are of critical interest, especially in the context of increasingly multiresistant pathogens [15]. However, in-depth studies in this field are only possible with measurement tools for the identification and characterization of the microbial composition. To avoid the complexity of unknown natural microbial communities, synthetic co-culture systems allow defined combinations of microorganisms with beneficial metabolic pathways. Yet, in contrast to natural communities, which are typically stabilized by natu...

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“…The flexibility of 3-D printing fabrication, in terms of design and manufacturing makes it possible to incorporate multiple sensing approaches such as electrochemical [16,23] or optical [17] methods on sensing probes. Electrical resistance measurement as a sensing mechanism can provide more information about phase transition than temperature measurement alone.…”

Section: Sensing Probes Produced With 3-d Printing For Monitoring Cryopreservation Proceduresmentioning

confidence: 99%

Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications

2019

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Cryopreservation has been recognized as a powerful tool for long-term preservation of genetic resources. However, the outcomes of cryopreservation by different user groups often vary due to inconsistency in procedures and freezing equipment. Herein, we report on the feasibility of providing customizable sensing probes with three-dimensional (3-D) printing to monitor cryopreservation phenomena. The objectives were to: 1) introduce 3-D printing as a fabrication method for developing customizable probes to be used in cryogenic applications; 2) design and fabricate an example of a 3-D printed sensing probe and multiplexer capable of detecting phasechange phenomena based on quantitative data regarding sample electrical resistance and temperature, and 3) demonstrate the sensing platform in cryopreservation conditions and in combination with a custom-made 3-D printed freezing device. The sensing probe developed was designed to fit within standard 0.5-ml French straws. Phase-transition phenomena were detected by analyzing electrical resistance changes. The quantitative data from this device in conjugation with a 3-D printed freezer rack provided cryopreservation capability with high reproducibility and offered an alternative to expensive programmable freezers. The use of 3-D printing provided flexibility to develop new sensing probes or modify existing designs based on specific needs. After initial prototyping, fabrication, and testing of 3-D printed sensing probes, particularly useful designs can lead to reduction of variation in performing standardized cryopreservation protocols.

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A hand-held fluorescent sensor platform for selectively estimating green algae and cyanobacteria biomass

Cited by 29 publications

References 33 publications

A field-deployable and handheld fluorometer for environmental water quality monitoring

A field-deployable and handheld fluorometer for environmental water quality monitoring

Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes

Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications

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