A Haploid Plant of the Potato Variety Katahdin

Hougas, R. W.; Peloquin, S. J.

doi:10.1038/1801209a0

Cited by 77 publications

(26 citation statements)

References 4 publications

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“…Parthenogenetic seed development has been routine in generating potato haploids (2n = 2x = 24) from tetraploid clones (Hougas & Peloquin, 1957 ;Montelongo-Escobedo & Rowe, 1969) and has also been extended to generating monoploids (n = x = 12) (Van Breukelen et al ., 1975) . The generation of inbred diploid clones could have important genetic and breeding implications ; i .e .…”

Section: Discussionmentioning

confidence: 99%

Genetic strategies to determine the mode of 2n egg formation in diploid potatoes

Douches

Quirós

1988

Euphytica

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In this study, nineteen diploid potato clones (Solanum spp . 2n = 2x = 24) were identified as 2n egg producers on the basis of fruit set in 2x-4x crosses . The segregation of three genes mapped close to the centromere, Got-1 (1 .1 cM), Pgm-2 (2 .0 cM), and Sdh-1 (8 .3 cM), were analyzed in the tetraploid offspring in these 2x-4x crosses to discriminate between First Division Restitution (FDR) and Second Division Restitution (SDR) modes of 2n egg formation . The co-dominant nature of these markers lead to more precise estimates of the recombinational frequencies as a result of completely classifying the segregating progenies . 2x-4x data revealed a predominance of SDR mechanisms occurring in 20 of the 21 families analyzed . With a SDR mode established, half-tetrad analysis (HTA) of four distal loci, 6-Pgdh-3, Mdh-1, Pgi-1, and Aps-1, revealed two SDR segregation patterns in some of the families . One pattern fit the expectations for the distal arm position . The gene-centromere map distances based upon SDR modes in the families following this pattern, were generally close to 4x-2x (FDR) estimates suggesting similar recombination rates between micro-and mega-sporogenesis . Heterozygosity transmission, on average, was 39 .1% . In the other segregation pattern, in which the diploid parents were derived from S. chacoense PI 230580, higher than expected homozygosity levels were found in the female 2n gametophyte populations . A post-meiotic doubling of the reduced megaspore, which generates homozygous 2n eggs, is suggested to operate in three families . The common genetic background of the diploid clones suggested a heritable nature of this mechanism . Pooled data from these three deviant families calculated that 1 .8% of the heterozygosity was transmitted to the tetraploid progeny .It is concluded that utilization of seven enzyme-coding loci, with previously established gene-centromere map distances, in 2x-4x crosses improved half-tetrad analysis (HTA) as a means to determine the mode of 2n gamete formation in megasporogenesis and megagametogenesis of diploid Solanum species.

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Section: Discussionmentioning

confidence: 99%

Genetic strategies to determine the mode of 2n egg formation in diploid potatoes

Douches

Quirós

1988

Euphytica

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“…Uniparental genome elimination leading to in vivo haploid production has been documented by plant breeders mostly from studies of interspecific hybridization experiments from several plant species [7][8][9][10][11][12] . In maize, an intraspecific in vivo haploid production system using Stock 6 genotype 13 derivatives is one of the commercially exploited haploid production system used for the development of hybrid maize cultivars 41 .…”

Section: M1 Haploidsmentioning

confidence: 99%

“…Arabidopsis thaliana and many crop species have proven to be recalcitrant to in vitro haploid production 5,6 . In some species, wide crosses [7][8][9][10][11][12] or intraspecific crosses to lines with specific genetic determinants 13,14 result in in vivo haploid embryo because the chromosomes of the haploid inducer (HI) are lost during postzygotic mitotic divisions while the chromosomes of the non-inducer parent are retained 15 . In a majority of such species, the efficacy of in vivo haploid induction has been hindered by the need for tedious embryo rescue protocols that require in vitro cell culture.…”

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confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

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“…(Tables 10, 11 and 12.) The early work of Hougas and Peloquin (1957, 1958a, 1958b directed attention to the potential use of dihaploids of tetraploid potatoes as material suitable for the simplification of genetic studies. By using S. phureja and other diploid species as pollen parents many di-haploids have been produced including plants containing the genes Rx, derived from U.S.D.A.…”

Section: Genetical Interpretationmentioning

confidence: 99%