A heat‐activated MAP kinase (HAMK) as a mediator of heat shock response in tobacco cells

Suri, Sarabjeet Singh; Dhindsa, Rajinder S.

doi:10.1111/j.1365-3040.2007.01754.x

Cited by 70 publications

(68 citation statements)

References 41 publications

(98 reference statements)

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“…The heat shock activated MAP kinase (HAMK) was identified as the first heat responsive MAP kinase in alfalfa cells . The activation of HAMK in tobacco cells was observed conjunctly with the accumulation of heat shock protein, HSP70, suggesting that HAMK may trigger a heat signaling cascade via HSP genes (Suri and Dhindsa 2008). The activation of HAMK also seems to be calcium dependent in tomato leaves and cells exposed to heat stress (Link et al 2002).…”

Section: Mapks and Temperature Stressesmentioning

confidence: 96%

MAPK cascades and major abiotic stresses

et al. 2014

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Plants have evolved with complex signaling circuits that operate under multiple conditions and govern numerous cellular functions. Stress signaling in plant cells is a sophisticated network composed of interacting proteins organized into tiered cascades where the function of a molecule is dependent on the interaction and the activation of another. In a linear scheme, the receptors of cell surface sense the stimuli and convey stress signals through specific pathways and downstream phosphorylation events controlled by mitogen-activated protein (MAP) kinases and second messengers, leading to appropriate adaptive responses. The specificity of the pathway is guided by scaffolding proteins and docking domains inside the interacting partners with distinctive structures and functions. The flexibility and the fine-tuned organization of the signaling molecules drive the activated MAP kinases into the appropriate location and connection to control and integrate the information flow. Here, we overview recent findings of the involvement of MAP kinases in major abiotic stresses (drought, cold and temperature fluctuations) and we shed light on the complexity and the specificity of MAP kinase signaling modules.

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Section: Mapks and Temperature Stressesmentioning

confidence: 96%

MAPK cascades and major abiotic stresses

et al. 2014

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“…While phosphorylation repressed HSF1-dependent transcriptional activation in human cells (Chu et al, 1996), multimerisation of yeast HSF in connection with hyperphosphorylation enhanced induction of target genes (Hashikawa and Sakurai, 2004). Heat-activated MAP kinases were shown to transduce heat stress signals by phosphorylating HSFs in tomato or promoting HSP gene expression in tobacco (Link et al, 2002;Suri and Dhindsa, 2008). Recently, phosphorylation and activation of Arabidopsis HSFA2 by MPK6 was reported (Evrard et al, 2013).…”

Section: Phosphorylation Of Hsfa4a By Interacting Mpk3 and Mpk6 Kinasesmentioning

confidence: 99%

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

et al. 2014

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Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradioldependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis.

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“…Recent work suggested that an unfolded protein response also exists in the chloroplast (Yu et al, 2012;Schmollinger et al, 2013;Ramundo et al, 2014). Plasma membrane fluidity has been identified as an important temperature sensor in land plants that appears to lie upstream of the unfolded protein response (Sangwan et al, 2002;Suri and Dhindsa, 2008;Saidi et al, 2009;Wu et al, 2012). Increased membrane fluidity appears to open calcium channels in the plasma membrane and inflowing calcium to trigger signaling cascades, including an H 2 O 2 burst (Königshofer et al, 2008), leading to the activation of the HS response.…”

Section: Introductionmentioning

confidence: 99%

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

et al. 2014

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We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42°C for 24 h and back to 25°C for ‡8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.

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A heat‐activated MAP kinase (HAMK) as a mediator of heat shock response in tobacco cells

Cited by 70 publications

References 41 publications

MAPK cascades and major abiotic stresses

MAPK cascades and major abiotic stresses

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

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