A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis

Abstract

Aims: The objective of this work was to purify the tyrosinase from Bacillus thuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. Methods and Results: A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this purity was measured under different conditions to study its kinetic characterizations. The optimum pH and thermal stability of this enzyme were also determined. The results revealed that the tyrosinase from Bt has distinct properties compared with those from other sources. Conclusions: A heat-inducible tyrosinase of a wild strain of Bt was identified and partially characterized. Significance and Impact of the Study: The distinct properties of Bt tyrosinase are important to the application of Bt as a biology pesticide.

Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from <i>Bacillus megaterium</i>

“…The identity of the bacteria was also confirmed by biochemical assays. To date, there are several reports on tyrosinase from B. thuringiensis strains [Dalfard et al, 2006;Liu et al, 2004;Ruan et al, 2005], and there is a putative tyrosinase gene located on a 208-kb plasmid of B. cereus 10987 [Rasko et al, 2004]. There is no description of a tyrosinase from B. megaterium , making tyrosinase-VS1 a novel enzyme.…”

“…The latter value suggests that the tyrosinase is active in the cell in the form of a monomer. Tyrosinases originating from Streptomyces nigrifaciens , Streptomyces glaucescens , P. putida F6 and B. thuringiensis are all monomers, while Vibrio tyrosinaticus, and T. roseum tyrosinases are dimers [Kong et al, 2000;Liu et al, 2004;McMahon et al, 2007].…”

“…lum, Pseudomonas maltophilia, Sinorhizobium meliloti , Marinomonas mediterranea, Thermomicrobium roseum, Bacillus thuringiensis , and Pseudomonas putida F6 [Claus and Decker, 2006;Dalfard et al, 2006;Liu et al, 2004;McMahon et al, 2007;Ruan et al, 2005]. Recently, a unique tyrosinase with a high tyrosine-hydroxylation/ dopa-oxidase ratio was discovered in Ralstonia solanacearum [Hernàndez-Romero et al, 2006].…”

Enhanced Production, Purification and Characterization of Alkaline Keratinase from <i>Streptomyces minutiscleroticus </i>DNA38

“…Influence of temperature: The activity of enzyme was determined by incubating the assay mixture at temperatures ranging from 10 to 100 ᵒC with an increment of 10 ᵒC. The reactions were performed at optimum pH value of the enzyme [24].…”

Evaluation of Tyrosinase Producing Indigenous Bacterial Strains With Reference to Their Efficacy In L-Dopa Production

“…This enzyme has both monophenolase and diphenolase activity and responsible for the biosynthesis of melanin. It has been produced from various sources such as Escherichia intermedia [6], Bacillus thuringiensis [7], Banana [8], Aspergillus oryzae [9], Portulaca grandiflora [10], Yarrowia lypolytica [11], Acremonium rutilum [12], Brevundimonas sp [13] and Bacillus sp JPJ [1] and Hypericum lariciforium juss [14] Although, it has been observed that very few attempts have been made to produce L-Dopa using bacterial sources. Therefore, the present study is undertaken to isolate and explore L-Dopa producing bacteria present in the soil.…”