A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

Liu, Qingcai; Deng, Suining; Liu, Baoshen; Tao, Yongfu; Ai, Haiyue; Liu, Jianju; Zhang, Yongzhong; Zhao, Yan; Xu, Ming

doi:10.1038/s41467-020-14372-3

Cited by 56 publications

(54 citation statements)

References 61 publications

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“…However, our understanding on the host factors responsible for RBSDV infection or plant defense is still limited. A recent report described that viral P7-1 recruited a host factor named Rab GDP dissociation inhibitor alpha (RabGDIα) for RBSDV infection in maize [2]. In this research, two copies of ZmAKINβγ, the regulatory subunit of SnRK1 complex were identified to interact with the P8 protein of RBSDV, and could respond to RBSDV infection in a similar response pattern.…”

Section: Discussionmentioning

confidence: 89%

“…However, a variety of diseases pose serious threats to maize production and quality. Among them, maize rough dwarf disease (MRDD) is a widespread and destructive viral disease worldwide [2,3]. It has been reported that the causal agents of MRDD in China are two fijiviruses (in the family of Reoviridae) including rice black streaked dwarf virus (RBSDV) and southern rice black-streaked dwarf virus (SRBSDV) [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection

Zhang

et al. 2020

Viruses

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Rice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried out using the viral P8 protein as a bait. ZmAKINβγ-1 and ZmAKINβγ-2 (βγ subunit of Arabidopsis SNF1 kinase homolog in maize) possessing high sequence similarities (encoded by two gene copies) were identified as interaction partners. Their interactions with P8 were confirmed in both Nicotiana benthamiana cells and maize protoplasts by bimolecular fluorescence complementation assay. The accumulation levels of ZmAKINβγ mRNAs were upregulated at the stage of the viral symptoms beginning to appear and then downregulated. ZmAKINβγs are putative regulatory subunits of the SnRK1 complex, a core regulator for energy homeostasis. Knockdown of ZmAKINβγs in maize regulated the expression levels of the genes involved in sugar synthesis or degradation, and also the contents of both glucose and sucrose. Importantly, downregulation of ZmAKINβγs expressions facilitated the accumulation of RBSDV in maize. These results implicate a role of ZmAKINβγs in the regulation of primary carbohydrate metabolism, and in the defense against RBSDV infection.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection

Zhang

et al. 2020

Viruses

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“…In addition, TEs of different types have been shown to alter gene splicing upon insertion. As an example, an Helitron insertion into a host susceptibility factor gene causes its alternative splicing leading to resistance to maize rough dwarf disease (Liu et al, 2020). Moreover, as noted above, TEs are the main target of epigenetic silencing, and therefore, the insertion of a TE within or close to a gene may bring the silencing machinery to this gene altering its expression (Zhang H. et al, 2018).…”

Section: Effects On Transposable Element Activitymentioning

confidence: 99%

Genomics of Evolutionary Novelty in Hybrids and Polyploids

2020

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It has long been recognized that hybridization and polyploidy are prominent processes in plant evolution. Although classically recognized as significant in speciation and adaptation, recognition of the importance of interspecific gene flow has dramatically increased during the genomics era, concomitant with an unending flood of empirical examples, with or without genome doubling. Interspecific gene flow is thus increasingly thought to lead to evolutionary innovation and diversification, via adaptive introgression, homoploid hybrid speciation and allopolyploid speciation. Less well understood, however, are the suite of genetic and genomic mechanisms set in motion by the merger of differentiated genomes, and the temporal scale over which recombinational complexity mediated by gene flow might be expressed and exposed to natural selection. We focus on these issues here, considering the types of molecular genetic and genomic processes that might be set in motion by the saltational event of genome merger between two diverged species, either with or without genome doubling, and how these various processes can contribute to novel phenotypes. Genetic mechanisms include the infusion of new alleles and the genesis of novel structural variation including translocations and inversions, homoeologous exchanges, transposable element mobilization and novel insertional effects, presence-absence variation and copy number variation. Polyploidy generates massive transcriptomic and regulatory alteration, presumably set in motion by disrupted stoichiometries of regulatory factors, small RNAs and other genome interactions that cascade from single-gene expression change up through entire networks of transformed regulatory modules. We highlight both these novel combinatorial possibilities and the range of temporal scales over which such complexity might be generated, and thus exposed to natural selection and drift.

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“…Powstające skrócone roślinne białko GDI nie tworzy kompleksu z białkiem wirusowym. Dzięki temu rośliny kukurydzy niosące ten allel są odporne na pierwotną infekcję wirusem RBSDV [50]. TBSV (ang.…”

Section: Odporność Roślin Na Patogeny I Interakcje Z Mikroorganizmamiunclassified

Funkcje białek Rab w roślinach – na poziomie komórki i organizmu

2021

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Białka Rab są niezbędne w procesach fuzji i pączkowania błon i w związku z tym są kluczowe dla regulacji transportu wewnątrzkomórkowego w komórkach eukariotycznych. Jednak białka Rab mają też wpływ na wiele innych aspektów funkcjonowania komórek, takich jak rearanżacje cytoszkieletu, wyznaczenie polarności komórki czy przekazywanie sygnałów. Białka Rab oddziałują tu zarówno pośrednio, poprzez swój wpływ na dostarczanie odpowiednich białek i materiałów do ich właściwych lokalizacji docelowych w komórce, jak i bezpośrednio, poprzez białka efektorowe. Konsekwencje zaburzeń w funkcjonowaniu białek Rab objawiają się w związku z tym na wszystkich poziomach działania organizmu - od komórek przez tkanki i organy aż po cały organizm. U roślin procesy zależne od białek Rab są istotne dla architektury komórki, jej różnicowania, reakcji na stres biotyczny i abiotyczny, a także dla wydajności produkcji rolniczej.

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A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

Cited by 56 publications

References 61 publications

Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection

Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection

Genomics of Evolutionary Novelty in Hybrids and Polyploids

Funkcje białek Rab w roślinach – na poziomie komórki i organizmu

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