A Heteromeric RNA-Binding Protein Is Involved in Maintaining Acrophase and Period of the Circadian Clock

Iliev, Dobromir; Voytsekh, Olga; Schmidt, Eva-Maria; Fiedler, Monika; Nykytenko, Alla; Mittag, Maria

doi:10.1104/pp.106.085944

Cited by 62 publications

(90 citation statements)

References 41 publications

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“…Both light conditions altered the levels of these transcripts, but in several cases the changes in transcript abundance were modest (1.5-to 3-fold). An exception was noted for the C3 subunit of the RNA binding protein CHLAMY1; altered expression of this protein causes phase shifts in circadian responses (Iliev et al, 2006). We observed an ;15-fold increase in the C3 transcript after the cells were exposed to 30 min of either blue or red light, demonstrating that the gene encoding C3 belongs to a group of rapidly induced genes such as VVD in Neurospora; a disruption in VVD also causes phase shifts of circadian rhythms (Heintzen et al, 2001;Lewis et al, 2002).…”

Section: Blue and Red Light Cause A Similar Change In Gene Expressionmentioning

confidence: 80%

“…The complete pKP39 sequence is presented in Supplemental Data Set 2 online. The mutant strain SAG73.72:acry1A was transformed with the AhdI-linearized pKP39 using the autolysin method as described earlier (Iliev et al, 2006). Cells were grown on Tris-acetate-phosphate agar medium (Harris, 1989) containing 20 µg/mL hygromycin B.…”

Section: Complementation Of the Acry1a Mutant By Transformation With mentioning

confidence: 99%

“…The preparation of C. reinhardtii crude protein extracts and the immunoblots to detect aCRY was performed according to Iliev et al (2006) with the following exceptions. Proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride (PVDF) membranes and blocked in a 5% suspension of powdered milk in Tris-buffered saline (pH 8.0) with 0.1% Tween 20.…”

Section: Crude Extracts and Immunoblotsmentioning

confidence: 99%

“…These clock-related proteins include the two subunits of the RNA binding protein CHLAMY1 (C1 and C3 subunits; Iliev et al, 2006), several Rhythm Of Chloroplast (ROC) proteins (Matsuo et al, 2008), Casein Kinase1 (CK1; Schmidt et al, 2006), and Constans (CO), which is also involved in photoperiodic signaling (Serrano et al, 2009). Among the ROCs are a number of putative transcription factors (ROC15, ROC40, ROC66, and ROC75), a putative subunit of E3 ubiquitin ligase (ROC114), and a protein of unknown function that contains leucine-rich repeats (ROC55).…”

Section: Introductionmentioning

confidence: 99%

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A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii

et al. 2012

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Cryptochromes are flavoproteins that act as sensory blue light receptors in insects, plants, fungi, and bacteria. We have investigated a cryptochrome from the green alga Chlamydomonas reinhardtii with sequence homology to animal cryptochromes and (6-4) photolyases. In response to blue and red light exposure, this animal-like cryptochrome (aCRY) alters the light-dependent expression of various genes encoding proteins involved in chlorophyll and carotenoid biosynthesis, light-harvesting complexes, nitrogen metabolism, cell cycle control, and the circadian clock. Additionally, exposure to yellow but not far-red light leads to comparable increases in the expression of specific genes; this expression is significantly reduced in an acry insertional mutant. These in vivo effects are congruent with in vitro data showing that blue, yellow, and red light, but not far-red light, are absorbed by the neutral radical state of flavin in aCRY. The aCRY neutral radical is formed following blue light absorption of the oxidized flavin. Red illumination leads to conversion to the fully reduced state. Our data suggest that aCRY is a functionally important blue and red light-activated flavoprotein. The broad spectral response implies that the neutral radical state functions as a dark form in aCRY and expands the paradigm of flavoproteins and cryptochromes as blue light sensors to include other light qualities.

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Section: Blue and Red Light Cause A Similar Change In Gene Expressionmentioning

confidence: 80%

Section: Complementation Of the Acry1a Mutant By Transformation With mentioning

confidence: 99%

Section: Crude Extracts and Immunoblotsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii

et al. 2012

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“…The O. tauri and C. reinhardtii genomes lack additional PRR-like genes and do not have recognizable ELF3, ELF4, GI, or ZTL homologs (Matsuo et al, 2008;Corellou et al, 2009). In addition, forward genetic studies in C. reinhardtii have demonstrated that genes without obvious homologs in higher plants are essential for normal clock function in this species (Iliev et al, 2006;Matsuo et al, 2008). It will be fascinating to learn the mechanistic similarities and differences between the clocks of the green algae and those of the land plants.…”

Section: The Beginning Of Timingmentioning

confidence: 99%

Plant Biology in the Fourth Dimension

Harmer

2010

Plant Physiology

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The nature of time has long been an obsession for philosophers, scientists, and laymen. This nearly universal interest in time is encapsulated by the fact that the quotation "Time is nature's way of keeping everything from happening at once" has been attributed to both Woody Allen and Albert Einstein! Although some argue that time is an illusion, ongoing work has revealed that most organisms possess an internal oscillator, the circadian clock, which has a pervasive influence on growth, development, and responses to the environment. Studies of circadian rhythms in plants reveal that because everything doesn't happen at once, plants have evolved sophisticated mechanisms to partition physiological processes so that they occur at the most advantageous times, both on seasonal and daily scales. A BRIEF HISTORY OF TIMINGIt is apparent to even the most casual observer that plant physiology is strongly influenced by time: for example, plants generally fix carbon during the day but are carbon consumers at night, and plant reproduction is strongly tied to the seasons. Although these changes in physiology are clearly linked to changes in the environment, they are also strongly influenced by the plant circadian clock. Studies spanning the past 300 years have revealed that many features of plant physiology are affected by the circadian clock (McClung, 2006). Clock-influenced processes range from once-in-a-lifetime events such as germination and the transition from vegetative to reproductive growth, to annual events such as the onset of flowering or winter dormancy, to daily processes such as rhythmic movements of petals and leaves and emission of floral fragrance. Ongoing, intensive investigation into clock-regulated processes are revealing that plants are even more sophisticated time keepers than we previously thought, with most or all aspects of plant physiology influenced by the circadian system. The molecular nature of the plant oscillator is now becoming clear, and the development of mathematical models describing this clock are raising hopes that we will someday be able to predict how particular environmental conditions will interact with a given genotype to shape plant growth and development in the real world.HOW DO PLANTS TELL TIME?

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Comparative Phosphoproteomics to Identify Targets of the Clock-Relevant Casein Kinase 1 in C. reinhardtii Flagella

Boesger

Wagner

Weisheit

et al. 2014

Methods in Molecular Biology

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In the green biflagellate alga Chlamydomonas reinhardtii different clock-relevant components have been identified that are involved in maintaining phase, period, and amplitude of circadian rhythms. It became evident that several of them are interconnected to flagellar function such as CASEIN KINASE1 (CK1). CK1 is involved in keeping the period. But it is also relevant for the formation of flagella, where it is physically located, and it controls the swimming velocity. In this chapter, we describe (1) how the flagellar sub-proteome is purified, (2) how phosphopeptides from this organelle are enriched, (3) how in vivo phosphorylation sites are determined, and (4) how direct and indirect flagellar targets of CK1 can be found using a specific inhibitor. Such a procedure can also be employed with other clock-relevant kinases if specific inhibitors or mutants are available.

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A Heteromeric RNA-Binding Protein Is Involved in Maintaining Acrophase and Period of the Circadian Clock

Cited by 62 publications

References 41 publications

A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii

A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii

Plant Biology in the Fourth Dimension

Comparative Phosphoproteomics to Identify Targets of the Clock-Relevant Casein Kinase 1 in C. reinhardtii Flagella

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