A High Amylose Wheat Diet Improves Gastrointestinal Health Parameters and Gut Microbiota in Male and Female Mice

Lim, See Meng; Choo, Jocelyn M.; Li, Hui; O’Rielly, Rebecca; Carragher, John; Rogers, Geraint B.; Searle, Iain; Robertson, Sarah A.; Page, Amanda J.; Mühlhäusler, Beverly S.

doi:10.3390/foods10020220

Cited by 11 publications

(5 citation statements)

References 71 publications

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“…Conversely, in line with this study, sex dependency of Turicibacter and Enterococcus genera in C57BJ/6 mice had been documented (Elderman et al, 2018). Although Faecalibaculum and Lactococcus abundance is decreased more markedly in females, both taxa have been reported to decline in both sexes of mice fed on highamylose wheat diet (Lim et al, 2021). However, such sex-specific effects are strikingly evident at high fiber doses, suggesting dosedependency as another variable for exerting universal benefits.…”

Section: Discussionsupporting

confidence: 84%

Dietary fiber modulates gut microbiome and metabolome in a host sex-specific manner in a murine model of aging

Kadyan,

Park,

Wang

et al. 2023

Front. Mol. Biosci.

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Emerging evidence reveals the fundamental role of the gut microbiome in human health. Among various factors regulating our gut microbiome, diet is one of the most indispensable and prominent one. Inulin is one of the most widely-studied dietary fiber for its beneficial prebiotic effects by positively modulating the gut microbiome and microbial metabolites. Recent research underscores sexual dimorphism and sex-specific disparities in microbiome and also diet-microbiome interactions. However, whether and how the prebiotic effects of dietary fiber differ among sexes remain underexplored. To this end, we herein examine sex-specific differences in the prebiotic effects of inulin on gut microbiome and metabolome in a humanized murine model of aging i.e., aged mice carrying human fecal microbiota. The findings demonstrate that inulin exerts prebiotic effects, but in a sex-dependent manner. Overall, inulin increases the proportion of Bacteroides, Blautia, and glycine, while decreasing Eggerthella, Lactococcus, Streptococcus, trimethylamine, 3-hydroxyisobutyrate, leucine and methionine in both sexes. However, we note sex-specific effects of inulin including suppression of f_Enteroccaceae:_, Odoribacter, bile acids, malonate, thymine, valine, acetoin, and ethanol while promotion of Dubosiella, pyruvate, and glycine in males. Whereas, suppression of Faecalibaculum, Lachnoclostridium, Schaedlerella, phenylalanine and enhancement of Parasutterella, Phocaeicola, f_Lachnospiraceae;_, Barnesiella, Butyricimonas, glycine, propionate, acetate and glutamate are observed in females. Altogether, the study reveals that prebiotic mechanisms of dietary fiber vary in a sex-dependent manner, underscoring the importance of including both sexes in preclinical/clinical studies to comprehend the mechanisms and functional aspects of dietary interventions for effective extrapolation and translation in precision nutrition milieus.

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Section: Discussionsupporting

confidence: 84%

Dietary fiber modulates gut microbiome and metabolome in a host sex-specific manner in a murine model of aging

Kadyan,

Park,

Wang

et al. 2023

Front. Mol. Biosci.

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“…Thus, this is the first study to look at the effect of RS on a rodent diet based on NHANES data ( 30 ). Furthermore, mice were fed with the RS diets for only 3 weeks while other rodent studies have used longer times ( 18 , 26 , 56 , 73 ), which may result in further changes to the microbiota. Although RS2-type starches share some basic characteristics, there may be subtle differences in their structures that can affect subsequent utilization by various bacterial species ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

Resistant Potato Starch Alters the Cecal Microbiome and Gene Expression in Mice Fed a Western Diet Based on NHANES Data

et al. 2022

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Several studies indicate that the four major types of resistant starch (RS1-4) are fermented in the cecum and colon to produce short-chain fatty acids (SCFAs) and can alter the microbiome and host physiology. However, nearly all these studies were conducted in rodents fed with a diet that does not approximate what is typically consumed by humans. To address this, mice were fed a Total Western Diet (TWD) based on National Health and Nutrition Examination Survey (NHANES) data that mimics the macro and micronutrient composition of a typical American diet for 6 weeks and then supplemented with 0, 2, 5, or 10% of the RS2, resistant potato starch (RPS), for an additional 3 weeks. The cecal microbiome was analyzed by 16S sequencing. The alpha-diversity of the microbiome decreased with increasing consumption of RPS while a beta-diversity plot showed four discreet groupings based on the RPS level in the diet. The relative abundance of various genera was altered by feeding increasing levels of RPS. In particular, the genus Lachnospiraceae NK4A136 group was markedly increased. Cecal, proximal, and distal colon tissue mRNA abundance was analyzed by RNASeq. The cecal mRNA abundance principal component analysis showed clear segregation of the four dietary groups whose separation decreased in the proximal and distal colon. Differential expression of the genes was highest in the cecum, but substantially decreased in the proximal colon (PC) and distal colon (DC). Most differentially expressed genes were unique to each tissue with little overlap in between. The pattern of the observed gene expression suggests that RPS, likely through metabolic changes secondary to differences in microbial composition, appears to prime the host to respond to a range of pathogens, including viruses, bacteria, and parasites. In summary, consumption of dietary RPS led to significant changes to the microbiome and gene expression in the cecum and to a lesser extent in the proximal and distal colon.

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“…Thus, adopting high‐amylose wheat flour might increase the daily intake of dietary fiber and bridge the fiber gap. In addition, it can impart several health benefits that can be claimed on product labels and thus attract health‐conscious consumers seeking high‐fiber and low carbohydrate foods 72 . Raising awareness about high‐amylose wheat flour will boost its uses 65 …”

Section: Starch Compositionmentioning

confidence: 99%

“…In addition, it can impart several health benefits that can be claimed on product labels and thus attract health-conscious consumers seeking high-fiber and low carbohydrate foods. 72 Raising awareness about high-amylose wheat flour will boost its uses. 65 Nevertheless, there are limited studies on the application of high-amylose durum wheat flour for food product development.…”

Section: Starch Compositionmentioning

confidence: 99%

Novel quality features to expand durum wheat applications

Aoun

Boukid

2022

J Sci Food Agric

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Durum wheat represents a staple food in the human diet owing to its nutritional and technological features. In comparison to common wheat, durum wheat has higher tolerance to biotic and abiotic stresses. However, its production and culinary uses are limited compared to common wheat. Therefore, significant attention was attributed to upgrading the key quality of durum wheat (i.e., hardness, protein, starch and color). This review intends to put the spotlight on the modification of these properties to create new functionalities suiting a wider range of food applications based on critical compilation of scientific publications. Targeting specific genes has been shown to be a valuable strategy to design novel wheat varieties with higher nutritional value (e.g., high amylose), improved technological properties (e.g., higher glutenin content), attractive appearance (e.g., colored wheat) and new uses (e.g., soft durum wheat for breadmaking). Further efforts are still needed to find efficient ways to stabilize and maintain these properties. © 2022 Society of Chemical Industry.

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A High Amylose Wheat Diet Improves Gastrointestinal Health Parameters and Gut Microbiota in Male and Female Mice

Cited by 11 publications

References 71 publications

Dietary fiber modulates gut microbiome and metabolome in a host sex-specific manner in a murine model of aging

Dietary fiber modulates gut microbiome and metabolome in a host sex-specific manner in a murine model of aging

Resistant Potato Starch Alters the Cecal Microbiome and Gene Expression in Mice Fed a Western Diet Based on NHANES Data

Novel quality features to expand durum wheat applications

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