A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP)

Gilbert, KG; Garton, Stephen; Karam, Mohamed; Arnold, G. M.; Karp, A.; Edwards, Keith J.; Cooke, D.J.; Barker, J. H. A.

doi:10.1007/s00122-001-0863-3

Cited by 33 publications

(31 citation statements)

References 20 publications

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“…On the average, the SAMPL marker yielded fewer polymorphic bands per primer combination than AFLP (44 vs. 47, respectively) but the percentage of polymorphism detected by SAMPL (95.3%) was higher than that detected by AFLP (85.6%). Molecular markers such as AFLP and SAMPL appear to be efficient tools for assessing genetic variation; they showed a very high level of polymorphism and confirmed previously reported data Gilbert et al 2002).…”

Section: Discussionsupporting

confidence: 80%

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Adaptability and variation in Isatis tinctoria L.: a new crop for Europe

Spataro

Negri

2007

Euphytica

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Isatis tinctoria L. was cultivated until the 19th century to produce indigo, a natural blue pigment used principally for dyestuffs. The current search for alternative crops and interest in natural products has led to reconsidering I. tinctoria as a crop to be grown in marginal areas to produce natural indigo. To reintroduce I. tinctoria into cultivation, its behaviour under different climatic conditions as well as its morpho-physiological and genetic diversity must be assessed in order to evaluate the possibilities of future breeding work. To do this, a Eurasian collection of 15 accessions was studied in a 2-year experiment. The study was carried out in four locations in order to assess plant performance at altitudes ranging from 380 to 1,700 m a.s.l. A second experiment evaluated the morpho-physiological diversity of several traits (some related to agronomic performances) of the collection. In a third experiment the genetic traits of the collection were characterised by using eight AFLP and eight SAMPL markers. The species showed a wide adaptability to different mountainous conditions and the populations showed high morphologic and genetic variability and differed according to their origins. Both morpho-physiological and molecular characterisation allowed the accessions to be distinguished into groups of European and Asian origin. Future breeding work is recommended because some accessions have good agronomic potential.

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Section: Discussionsupporting

confidence: 80%

“…Molecular markers can be used to determine the genetic diversity between and within species. Gilbert et al (2002) showed that AFLP (Amplified Fragment Length Polymorphisms) molecular markers can be used to distinguish different species (I. tinctoria, I. indigotica Fort. and I. glauca Aucher ex Boiss) and landraces of I. tinctoria.…”

Section: Introductionmentioning

confidence: 99%

Adaptability and variation in Isatis tinctoria L.: a new crop for Europe

Spataro

Negri

2007

Euphytica

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“…Gilbert et al (2002)showed that AFLP (Amplified Fragment Length Polymorphisms) molecular markers can be used to distinguish different species (I. tinctoria, I. indigotica Fort. and I. glauca Aucher ex Boiss.)…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation and Population Structure in a Eurasian Collection of Isatis tinctoria L.

Spataro

Taviani

Negri

2006

Genet Resour Crop Evol

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Isatis tinctoria L. is a biennial species which was cultivated in Europe until the 18th century to produce indigo, a pigment used for dyestuffs. Today there is renewed interest in this ancient crop because of a market demand for natural dyes. Cultivation of the species appears to be particularly suitable for marginal areas. Information about the evolutionary and genetic patterns of I. tinctoria is needed if varieties or to be developed in future breeding programs. The aim of this study was to assess the genetic variation and similarity levels among and within natural populations of I. tinctoria from Europe and central Asia. Fifteen populations were used to carry out the genetic analyses with AFLP and SAMPL molecular markers. Data collected were analysed by the UPGMA method and were used to perform AMOVA. The results are consistent with the hypothesis that the crop originated in an eastern centre of origin and moved westward giving rise to a gene pool that is quite different from the original. The wide within-population variation revealed by this study suggests that effective breeding work to develop varieties suitable for marginal environments can be carried out easily.

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“…AFLP fragments, present exclusively in only one species or landrace, have been amplified for Isatis spp. (Gilbert et al 2002) also. Species-specific alleles have also been detected in poplars using microsatellite markers (Rahman and Rajora 2002).…”

Section: Discussionmentioning

confidence: 92%

Screening interspecific hybrids of Populus (P. ciliata × maximowiczii) using AFLP markers

et al. 2003

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Hybrids of Populus ciliata x maximowiczii are very vigorous and outperform both the parents in growth performance and yield. Genetic evaluation of 24 of these interspecific hybrids along with the two mother trees ( Populus ciliata), and five male-parent ( Populus maximowiczii) genotypes was carried out using the AFLP marker assay. Eight AFLP primer combinations detected 428 markers, of which 280 (66%) were polymorphic. Genetic relationships within the samples were evaluated by generating the similarity matrix based on Jaccard's coefficient. The phenetic dendrograms, as well as the PCO plots, separated the hybrids and the two parent species into three distinct clusters. The hybrids grouped closer to the P. ciliata (female parent) cluster as compared to the P. maximowiczii (male parent) cluster. The hybrid cluster contained internal groupings, which correlated to some extent with growth performance. The four best performing hybrids (42m1, 65m1, 23m2, Cm2-5-20/91) formed a distinct sub-cluster. Data from a single primer combination was sufficient for distinguishing the hybrids from the parents and assigning paternity. The hybrids showed 22 markers that were absent in P. ciliata but were monomorphically present in all the hybrids, suggesting outcrossing and common paternity. Further, these 22 markers were found in all the P. maximowiczii genotypes confirming it as the male parent. These male-specific markers can be converted to SCAR markers and used for rapid screening of the P.ciliata x maximowiczii hybrids. The primer combination E-AAC x M-CAA was identified as most suitable for ascertaining true hybridity. AFLP proves to be a useful tool for screening of P. ciliata x maximowiczii hybrids at the early stages of development.

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A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP)

Cited by 33 publications

References 20 publications

Adaptability and variation in Isatis tinctoria L.: a new crop for Europe

Adaptability and variation in Isatis tinctoria L.: a new crop for Europe

Genetic Variation and Population Structure in a Eurasian Collection of Isatis tinctoria L.

Screening interspecific hybrids of Populus (P. ciliata × maximowiczii) using AFLP markers

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