A high-density integrated odorant sensor array system based on insect cells expressing insect odorant receptors

Nagata, Sanato; Kameshiro, Norifumi; Terutsuki, Daigo; Mitsuno, Hidefumi; Sakurai, Takeshi; Niitsu, Kiichi; Nakazato, Kazuhiko; Kanzaki, Ryohei; Ando, Masahiko

doi:10.1109/memsys.2018.8346540

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Potentiometric gas sensors are another group of electrochemical devices, which measure changes in the potential or electric field upon interaction with vapor gases. These devices have also been widely employed for odor monitoring purposes [ 91 , 92 ]. Potentiometric gas sensors can be deployed in a cell-based configuration similar to amperometric devices, using two or more electrodes in contact with an electrolyte.…”

Section: Gas Sensors For Vocs Detectionmentioning

confidence: 99%

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Ollé

Farré-Lladós

Casals‐Terré

2020

Sensors

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In recent years, advancements in micromachining techniques and nanomaterials have enabled the fabrication of highly sensitive devices for the detection of odorous species. Recent efforts done in the miniaturization of gas sensors have contributed to obtain increasingly compact and portable devices. Besides, the implementation of new nanomaterials in the active layer of these devices is helping to optimize their performance and increase their sensitivity close to humans’ olfactory system. Nonetheless, a common concern of general-purpose gas sensors is their lack of selectivity towards multiple analytes. In recent years, advancements in microfabrication techniques and microfluidics have contributed to create new microanalytical tools, which represent a very good alternative to conventional analytical devices and sensor-array systems for the selective detection of odors. Hence, this paper presents a general overview of the recent advancements in microfabricated gas sensors and microanalytical devices for the sensitive and selective detection of volatile organic compounds (VOCs). The working principle of these devices, design requirements, implementation techniques, and the key parameters to optimize their performance are evaluated in this paper. The authors of this work intend to show the potential of combining both solutions in the creation of highly compact, low-cost, and easy-to-deploy platforms for odor monitoring.

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Section: Gas Sensors For Vocs Detectionmentioning

confidence: 99%

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Ollé

Farré-Lladós

Casals‐Terré

2020

Sensors

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“…Moreover, such precise visualization of the sample–substrate interface has the advantage of assessing biological interactions such as focal adhesion assembly , and physical interaction , such as repulsion of glycoconjugates (dense glycocalyx, composed of glycolipids, glycoproteins, etc.). Recently, we have developed “insect” cell-coupled FET devices with high selectivity for odorant molecules through the strong expression of odorant receptors (OR). , Minor structural differences in similar silk moth pheromone components (i.e., bombykol and bombykal) were sensitively discriminated based on drain current modulations of the sensors at room temperature. Previously, a scanning electron microscope (SEM) has clearly visualized dehydrated Spodoptera frugiperda (Sf21) cells in close contact with a sensor surface .…”

mentioning

confidence: 99%

“…Recently, we have developed “insect” cell-coupled FET devices with high selectivity for odorant molecules through the strong expression of odorant receptors (OR). 17 , 18 Minor structural differences in similar silk moth pheromone components (i.e., bombykol and bombykal) were sensitively discriminated based on drain current modulations of the sensors at room temperature. Previously, a scanning electron microscope (SEM) has clearly visualized dehydrated Spodoptera frugiperda (Sf21) cells in close contact with a sensor surface.…”

mentioning

confidence: 99%

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

Matsuzaki

Terutsuki

Sato

et al. 2022

J. Phys. Chem. Lett.

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Cell-coupled field-effect transistor (FET) biosensors have attracted considerable attention because of their high sensitivity to biomolecules. The use of insect cells (Sf21) as a core sensor element is advantageous due to their stable adhesion to sensors at room temperature. Although visualization of the insect cell–substrate interface leads to logical amplification of signals, the spatiotemporal processes at the interfaces have not yet been elucidated. We quantitatively monitored the adhesion dynamics of Sf21 using interference reflection microscopy (IRM). Specific adhesion signatures with ring-like patches along the cellular periphery were detected. A combination of zeta potential measurements and lectin staining identified specific glycoconjugates with low electrostatic potentials. The ring-like structures were disrupted after cholesterol depletion, suggesting a raft domain along the cell periphery. Our results indicate dynamic and asymmetric cell adhesion is due to low electrostatic repulsion with fluidic sugar rafts. We envision the logical design of cell–sensor interfaces with an electrical model that accounts for actual adhesion interfaces.

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Design and analysis of a three-dimensional millimeter-wave frequency-shift based CMOS biosensor using vertically stacked spiral inductors in LC oscillators

Matsunaga

Nakanishi

Kobayashi

et al. 2018

Analog Integr Circ Sig Process

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A high-density integrated odorant sensor array system based on insect cells expressing insect odorant receptors

Cited by 3 publications

References 12 publications

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Advancements in Microfabricated Gas Sensors and Microanalytical Tools for the Sensitive and Selective Detection of Odors

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

Design and analysis of a three-dimensional millimeter-wave frequency-shift based CMOS biosensor using vertically stacked spiral inductors in LC oscillators

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