A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers

Bert, Pierre‐François; Charmet, G.; Sourdille, Pierre; Hayward, M. D.; Balfourier, François

doi:10.1007/s001220051256

Cited by 150 publications

(117 citation statements)

References 21 publications

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“…Of the 506 markers, 73 were also polymorphic in an F 2 mapping family described elsewhere . While AFLP marker distribution can be clustered (Bert et al 1999), we found that the 73 mapped markers were distributed fairly randomly on each of the seven linkage groups of L. perenne (between 6 and 17 per linkage group). If extrapolated to the unmapped markers, it would be between 40 and 120 per linkage group.…”

Section: Polymorphism Type Lpcai Lphd1mentioning

confidence: 80%

Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

Skøt

Humphreys

et al. 2007

Genetics

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We describe a candidate gene approach for associating SNPs with variation in flowering time and watersoluble carbohydrate (WSC) content and other quality traits in the temperate forage grass species Lolium perenne. Three analysis methods were used, which took the significant population structure into account. First, a linear mixed model was used enabling a structured association analysis to be incorporated with the nine populations identified in the structure analysis as random variables. Second, a within-population analysis of variance was performed. Third, a tree-scanning method was used, in which haplotype trees were associated with phenotypes on the basis of inferred haplotypes. Analysis of variance within populations identified several associations between WSC, nitrogen (N), and dry matter digestibility with allelic variants within an alkaline invertase candidate gene LpcAI. These associations were only detected in material harvested in one of the two years. By contrast, consistent associations between the L. perenne homolog (LpHD1) of the rice photoperiod control gene HD1 and flowering time were identified. One SNP, in the immediate upstream region of the LpHD1 coding sequence (C-4443-A), was significant in the linear mixed model. Within-population analysis of variance and tree-scanning analysis confirmed and extended this result to the 2118 polymorphisms in some of the populations. The merits of the tree-scanning method are compared to the single SNP analysis. The potential usefulness of the 4443 SNP in marker-assisted selection is currently being evaluated in test crosses of genotypes from this work with turf-grass varieties.

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Section: Polymorphism Type Lpcai Lphd1mentioning

confidence: 80%

Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

Skøt

Humphreys

et al. 2007

Genetics

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“…The detection of microsatellites using a non-radioactive silver nitrate staining method was described by Tixier et al (1997). Some amplified fragment length polymorphisms (AFLPs) markers obtained following the procedure described in Bert et al (1999) were also added to the map.…”

Section: Molecular Analysismentioning

confidence: 99%

Molecular and physical mapping of genes affecting awning in wheat

et al. 2002

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Quantitative trait loci (QTL) for three traits related to awning (awn length at the base, the middle and the top of the ear) in wheat were mapped in a doubled-haploid line (DH) population derived from the cross between the cultivars ÔCourtotÕ (awned) and ÔChinese SpringÕ (awnless) and grown in Clermont-Ferrand, France, under natural field conditions. A molecular marker linkage map of this cross that was previously constructed based on 187 DH lines and 550 markers was used for the QTL mapping. The genome was well covered (more than 95%) and a set of anchor loci regularly spaced (one marker every 20.8 cM) was chosen for marker regression analysis. For each trait, only two consistent QTL were identified with individual effects ranging from 8.5 to 45.9% of the total phenotypic variation. These two QTL cosegregated with the genes Hd on chromosome 4A and B2 on chromosome 6B, which are known to inhibit awning. The results were confirmed using ÔChinese SpringÕ deletion lines of these two chromosomes, which have awned spikes, while ÔChinese SpringÕ is usually awnless. No quantitative trait locus was detected on chromosome 5A where the B1 awn-inhibitor gene is located, suggesting that both ÔCourtotÕ and ÔChinese SpringÕ have the same allelic constitution at this locus. The occurrence of awned speltoid spikes on the deletion lines of this chromosome suggests that ÔChinese SpringÕ and ÔCourtotÕ have the dominant B1 allele, indicating that B1 alone has insufficient effect to induce complete awn inhibition.

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“…Moreover, molecular markers such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) (Hayward et al, 1994;Hayward et al, 1998), amplified fragment length polymorphism (AFLP) (Bert et al, 1999), and simple sequence repeat (SSR) markers (Jones et al, 2001;Jones et al, 2002) were used to examine population genetic structure and diversity, and cultivar identification. They were also used to correlate molecular findings with morpho-agronomical traits (Geleta et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Ghariani¹,

Elazreg²,

Chtourou-Ghorbel³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic diversity estimation of ryegrass germplasm after screening 20 spontaneous accessions, including a local and an introduced cultivars. Same mean polymorphism information content values were obtained (0.37) for RAPD and ISSR suggesting that both marker systems were equally effective in determining polymorphisms. The average pairwise genetic distance values were 0.57 (morpho-agronomical traits), 0.68 (RAPD), and 0.51 (ISSR) markers data sets. A higher Shannon diversity index was obtained with ISSR marker (0.57) than for RAPD (0.54) and morphoagronomical traits (0.36). The Mantel test based on genetic distances of a combination of molecular markers and morpho-agronomical data exhibited (2015) a significant correlation between RAPD and ISSR data, suggesting that the use of a combination of molecular techniques was a highly efficient method of estimating genetic variability levels among Tunisian ryegrass germplasm. In summary, results showed that combining molecular and morpho-agronomical markers is an efficient way in assessing the genetic variability among Tunisian ryegrass genotypes. In addition, the combined analysis provided an exhaustive coverage for the analyzed diversity and helped us to identify suitable accessions showed by Beja and Jendouba localities, which present large similarities with cultivated forms and can be exploited for designing breeding programmes, conservation of germplasm and management of ryegrass genetic resources.

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A high-density molecular map for ryegrass (Lolium perenne) using AFLP markers

Cited by 150 publications

References 21 publications

Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.)

Molecular and physical mapping of genes affecting awning in wheat

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

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