A High‐Fat Diet Rich in Saturated and Mono‐Unsaturated Fatty Acids Induces Disturbance of Thyroid Lipid Profile and Hypothyroxinemia in Male Rats

Zhang, Xiaohan; Chen, Wenbin; Shao, Shanshan; Xu, Guowang; Song, Yuelin; Xu, Chao; Gao, Ling; Hu, Chunxiu; Zhao, Jiajun

doi:10.1002/mnfr.201700599

Cited by 30 publications

(35 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study demonstrated low levels of T3 and T4 in rats fed HFD, which indicates hypothyroxinemia. This finding supports a recent report that increased intake of an HFD and increased serum lipid fractions caused disturbances in the thyroid lipid profile and hypothyroxinemia and increased TSH levels in response to decreased thyroid hormones [38], which was ameliorated by dietary modification [39]. Maintaining thyroid homeostasis is essential for human health.…”

Section: Discussionsupporting

confidence: 90%

Moringa oleifera leaf extract ameliorated high-fat diet-induced obesity, oxidative stress and disrupted metabolic hormones

Othman

Amer²,

Basos

et al. 2019

Clin Phytosci

View full text Add to dashboard Cite

Background: Obesity is a health problem in many countries, and maintaining a perfect weight is challenging. Moringa oleifera leaf extract (ME) is rich in polyphenols with antioxidant and pharmaceutical potential. The present study investigated the potential protective effect of Moringa oleifera leaf extract against obesity induced from a high-fat diet (HFD), oxidative stress and disruption of metabolic hormones compared to simvastatin (SIM) or their combination. Results: Rats fed a HFD for 6 weeks exhibited a significant increase in body weight and levels of serum glucose and lipid fractions, verifying an obesity state. There were also higher levels of insulin and leptin and lower gherlin in sera of HFD rats compared to the levels in control rats. Homeostasis model assessment for insulin resistance (HOMA-IR), quantitative insulin sensitivity check index (QUICKI) and the atherogenic index were elevated, indicating the development of insulin resistance and dyslipidaemia in these rats. These changes were accompanied with a significant increase in oxidative stress, as indicated by elevated lipid peroxidation and protein oxidation with low levels of antioxidants in liver. The activities of liver function enzymes, including aspartate amino transferase, alanine amino transferase, alkaline phosphatase and gamma glutamyltransferase, were also significantly increased in serum. Concurrent treatment with 300 mg/kg ME for 6 weeks ameliorated the increase in body weight and improved the levels of glucose, lipid fractions and metabolic hormones, indicating the anti-obesity effect and amelioration of tissue insulin resistance potential of ME. ME treatment also normalized oxidative stress and antioxidants in liver and improved liver function enzymes, indicating the antioxidant potential of ME. The effects of ME were similar to SIM, and the combination of these agents was better than each agent alone. Conclusion: We propose that ME extract has anti-obesity and antioxidant potential and may be used as a lipidlowering drug to control weight, obesity and its pathophysiological consequences.

show abstract

Section: Discussionsupporting

confidence: 90%

Moringa oleifera leaf extract ameliorated high-fat diet-induced obesity, oxidative stress and disrupted metabolic hormones

Othman

Amer²,

Basos

et al. 2019

Clin Phytosci

View full text Add to dashboard Cite

show abstract

“…It is known that SFAs may induce development and affect progression of many chronic diseases through inflammatory response 27 . Two recent studies showed that high-fat diet causes thyroid dysfunction in rats and also induces hypothyroidism by decreasing total T4 and fT4 with increasing TSH levels 28,29 . These studies suggested that excess intake of animal fat contributes to pathogenesis of hypothyroidism.…”

Section: Discussionmentioning

confidence: 99%

Differences in food consumption between patients with Hashimoto’s thyroiditis and healthy individuals

Kaličanin

Brčić

Ljubetić

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Food is considered as important environmental factor that plays a role in development of Hashimoto's thyroiditis (HT). The goal of our study was to identify food groups, assessed by food frequency questionnaire, that differ in consumption frequency between 491 patients with HT and 433 controls. We also analysed association of food groups with the wealth of HT-related clinical traits and symptoms. We found significantly increased consumption of animal fat (OR 1.55, p < 0.0001) and processed meat (OR 1.16, p = 0.0012) in HT cases, whereas controls consumed significantly more frequently red meat (OR 0.80, p < 0.0001), non-alcoholic beverages (OR 0.82, p < 0.0001), whole grains (OR 0.82, p < 0.0001) and plant oil (OR 0.87, p < 0.0001). We also observed association of plant oil consumption with increased triiodothyronine levels in HT patients (β = 0.07, p < 0.0001), and, association of olive oil consumption with decreased systolic blood pressure (β = − 0.16, p = 0.001) in HT patients on levothyroxine (LT4) therapy. Analysis of food consumption between HT patients with and without LT4 therapy suggest that patients do not tend to modify their diet upon HT diagnosis in our population. Our study may be of relevance to nutritionists, nutritional therapists and clinicians involved in developing dietary recommendations for HT patients. Environmental factors, in addition to genetic factors, play important role in Hashimoto's thyroiditis (HT) development 1. HT is a chronic autoimmune thyroid disorder (AITD) characterized by the production of thyroid autoantibodies against thyroid peroxidase (TPOAb) and thyroglobulin (TgAb) 2. Other main characteristics of HT include infiltration of lymphocytes and ruination of thyroid tissue, which usually leads to hypothyroidism 3. This disease predominantly affects female population and is considered as one of the most frequent endocrine disorders 4. The estimated incidence of HT is 350/100,000 for females and 80/100,000 per year for males 5. It is most often diagnosed in women between the age of 30 and 60 6. High iodine intake (median urinary iodine concentration ≥ 300 μg/L) 7 , especially in areas with sufficient iodine supply, is one of the most known factors that increase the incidence of HT 8. Also, low vitamin D levels (below 30 ng/mL) 9 and low selenium intake (bellow 40 µg/day) 10 are proposed to be risk factors for HT development, albeit a role of these factors in HT still needs to be confirmed. Conversely, alcohol intake was proposed to decrease the risk for HT 11. Other dietary factors are also considered as potential environmental modifiers of the clinical appearance of HT 12-15. A diet low in gluten has been recently proposed to have beneficial effects on the disease course in a study of 34 women with HT 13. Another study of 83 patients with HT indicated that restriction of lactose intake in lactose-intolerant HT patients resulted with decreased thyroid-stimulating hormone (TSH) levels 14. However, the current knowledge of this topic is scarce and only the following two studies ...

show abstract

“…In present study, a HFD-induced hypothyroxinemia rat model was constructed according to our previous studies [24,25]. Excess intake of dietary fat induced significant thyroid dysfunction and hypothyroxinemia in rats by decreasing the expression of thyroid hormone synthesis-related proteins, providing evidence for the correlation between lipid profiles and organ function.…”

Section: Discussionmentioning

confidence: 99%

“…We compared the protein expression profile and succinylation level in the rat thyroid tissue between a high-fat diet (HFD) study group and a chow-diet (CD) control group. The experiment procedures consisted of four key steps as follows: (1) the establishment and sample collection in a hypothyroxinemia rat model, as previously described [ 24 , 25 ]; (2) label-free-based quantitative proteomics, including protein extraction, trypsin digestion, high-performance liquid chromatography (HPLC) fractionation, and antibody-based affinity enrichment of lysine succinylated peptides; (3) LC-MS/MS analyses; and (4) bioinformatics analyses. Three biological replicates were performed for the global proteome and succinylome analyses.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies [ 24 , 25 ] have found that excess intake of dietary fat induced decreased serum TT 4 and FT 4 concentrations in parallel with elevated serum TSH concentration, as well as abnormal morphology and lipid profile change of the thyroid gland, providing evidence for the correlation between lipid profiles and organ function, as well as a new prospect for understanding the pathogenesis of hypothyroidism. However, the underlying molecular mechanism remains unclear and needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats

Zhao

Luo

et al. 2020

International Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Hypothyroidism is a common disease, and its molecular mechanism still needs further investigation. Lysine succinylation is found to be involved in various metabolic processes associated with hypothyroidism. We performed quantitative analysis on lysine succinylome in thyroids of rats with hypothyroxinemia, which was induced through the administration of a high-fat diet. Overall, 129 differentially expressed proteins were quantified. Downregulated proteins were enriched in the thyroid hormone synthesis and thyroid hormone signaling pathways and were mainly localized in the mitochondria. In addition, 172 lysine succinylation sites on 104 proteins were obviously changed. Decreased succinylated proteins were involved in diverse metabolic pathways and were primarily localized in mitochondria. Finally, the mitochondrial oxygen consumption rates of human normal thyroid epithelial cells were measured to further verify the role of lysine succinylation. The mitochondrial oxygen consumption rates were markedly blunted in the cells treated with palmitic acid (all p<0.05), and the changes were reversed when the cells were treated with palmitic acid and desuccinylase inhibitor together (all p<0.05). Thus, we theorize that the thyroid differentially expressed proteins and changed succinylation levels played potential roles in the mitochondria-mediated energy metabolism in the high-fat diet-induced hypothyroxinemia rat model.

show abstract

A High‐Fat Diet Rich in Saturated and Mono‐Unsaturated Fatty Acids Induces Disturbance of Thyroid Lipid Profile and Hypothyroxinemia in Male Rats

Abstract: Excess intake of dietary fat induces disturbance of thyroid lipid profile and hypothyroxinemia, indicating thyroid dysfunction. We speculate that it may provide a new prospect in understanding the pathogenesis of hypothyroidism.

Cited by 30 publications

References 26 publications

Moringa oleifera leaf extract ameliorated high-fat diet-induced obesity, oxidative stress and disrupted metabolic hormones

Moringa oleifera leaf extract ameliorated high-fat diet-induced obesity, oxidative stress and disrupted metabolic hormones

Differences in food consumption between patients with Hashimoto’s thyroiditis and healthy individuals

Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats

Contact Info

Product

Resources

About