A high oocyte yield for intracytoplasmic sperm injection treatment is associated with an increased chromosome error rate

Haaf, Thomas; Hahn, Antje; Lambrecht, Anne; Grossmann, Bärbel; Schwaab, Eva; Khanaga, O.; Hahn, Thomas von; Tresch, Achim; Schorsch, Martin

doi:10.1016/j.fertnstert.2008.01.012

Cited by 45 publications

(29 citation statements)

References 21 publications

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“…In our study, the FSH dose and the number of retrieved oocytes had a negative impact on the TE quality. Our results are in agreement with recent studies that have shown that milder ovarian stimulation reduces the likelihood of segregation errors during early embryo cleavages [16,22] and results in high-quality embryos for transfer, as indicated by good embryo morphology [17]. The observation that higher FSH doses and oocyte yields are associated with impaired blastocyst development may be due to an supraphysiologic ovarian stimulation that interferes with the cell machinery during oocyte formation.…”

Section: Discussionsupporting

confidence: 92%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To identify the correlation between different cycles, patient factors and blastocyst characteristics. Methods The study included 420 patients undergoing ICSI cycles and 2781 graded blastocysts, which took into account the blastocyst quality. The correlations between the blastocyst parameters and the patient and cycle characteristics were assessed. Results The blastocyst development was negatively correlated with the maternal age, BMI and dose of FSH. The ICM was negatively correlated with the FSH dose, whereas the TE quality was influenced by the FSH dose, the maternal age and the number of retrieved oocytes. The embryo morphology on days two and three may predict the blastocyst developmental competence. Conclusions Older patients and patients with high BMI should not be included in extended embryo culture programmes. The extended culture may not favour embryos with poor morphology on days two and three of development. Additionally, a lower ovarian stimulation and decreased oocyte yields may lead to the development of high-quality blastocysts.

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Section: Discussionsupporting

confidence: 92%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

J Assist Reprod Genet

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“…However, Haaf et al (2009) also found chromosomal errors in fresh oocytes from young infertile patients (≤35 years) at a rate of Safety of human oocyte vitrification 34.4% in their first cycle or an increased rate of 43.8% in subsequent cycles. The oocytes of young donors (26.1 years) with no know fertility problems revealed an aneuploidy rate of only 19.1% (Velilla et al, 2013).…”

Section: Chromosomal Meiotic Constitutionmentioning

confidence: 94%

“…It is known that oocytes from women of advanced maternal age (.40 years) have a high rate of chromosomal abnormalities (≥50%) after FISH analysis (usually testing five chromosomes: 13, 16, 18, 21 and 22) (Haaf et al, 2009). However, Haaf et al (2009) also found chromosomal errors in fresh oocytes from young infertile patients (≤35 years) at a rate of Safety of human oocyte vitrification 34.4% in their first cycle or an increased rate of 43.8% in subsequent cycles.…”

Section: Chromosomal Meiotic Constitutionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

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“…Similarly, Haaf et al (27) demonstrated that the chromosome error rate was higher when more oocytes were retrieved. Women underwent a long GnRH agonist protocol with 112.5-225.0 IU of FSH per day.…”

Section: Mild Stimulationmentioning

confidence: 89%

Mild/minimal stimulation for in vitro fertilization: an old idea that needs to be revisited

Zarek

Muasher

2011

Fertility and Sterility

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A high oocyte yield for intracytoplasmic sperm injection treatment is associated with an increased chromosome error rate

Cited by 45 publications

References 21 publications

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Mild/minimal stimulation for in vitro fertilization: an old idea that needs to be revisited

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