The nonlinear evolution process of new vortex structures at the late-stage of the transition, including the 3-D spatial structure of barrel-shaped vortex and "dark spots" structure observed by experiment research, has been confirmed by our computational results. The formation mechanisms of these structures have been explored. It is revealed that the new vortex structures, the ring-like vortex chain and induced disturbance velocities play a dominant role in the generation of turbulent spots. boundary layers, transition, barrel-shaped vortex, turbulent spots, direct numerical simulation PACS: 47.27.Cn, 47.27