A high-performance liquid chromatographic method for the evaluation of aldrin epoxidation by cytochrome P-450 dependent monooxygenase in small liver samples

Pantaleoni, Giancarlo; Palumbo, Giancarlo; Fanini, D.; Giorgi, Raffaele De; Carlucci, G.; Sponta, A.M.

doi:10.1016/0731-7085(89)80125-6

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…To measure the amount of free dieldrin in the supernatant, HPLC was used (Pantaleoni et al 1989). Chromatography was conducted on a Waters HPLC device equipped with a 717plus Autosampler, model 1525 binary pumps, model 2487 Duel Absorbance Detector, and a Symmetry ® C18 5-µm (4.6 × 150 mm) column.…”

Section: In Vitro Analysis: Isothermal Adsorptionmentioning

confidence: 99%

Strong Adsorption of Dieldrin by Parent and Processed Montmorillonite Clays

Hearon

Wang

Phillips

2020

Enviro Toxic and Chemistry

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Widespread use of pesticides has resulted in the accumulation of pesticide residues in the environment due to their persistence and stability. To reduce potential exposures, we have developed broad‐acting clay‐based sorbents that can be included in the diet as enterosorbents to reduce the bioavailability and toxicity of chemicals. In the present study, parent and acid‐processed calcium montmorillonite clays (CM and APM, respectively) were used to determine their potential as sorbents of the organochlorine insecticide dieldrin. We used adsorption isotherms, thermodynamics, and dosimetry studies to determine the capacities and affinities of the clays, the enthalpies of the binding reactions, and potential doses of sorbent that could protect against high exposures. Adsorption isotherms for APM fit a Langmuir model with high enthalpy (suggesting chemisorption) and high capacity (Qmax value = 0.45 mol kg–1), indicating tight binding of dieldrin. Cultures of Hydra vulgaris were used to determine the ability of sorbents to protect a living organism from dieldrin toxicity. The inclusion of acid‐processed clays resulted in the highest reduction of dieldrin toxicity (70%) in the hydra. Further work indicated that both CM and APM can significantly reduce the bioavailability of dieldrin from soil (p ≤ 0.01). These results suggest that APM (and similar clays) can be effective sorbents of dieldrin and may be included in the diet and/or soil to protect against environmental exposures. Environ Toxicol Chem 2020;39:517–525. © 2019 SETAC

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Section: In Vitro Analysis: Isothermal Adsorptionmentioning

confidence: 99%