A High-Performance Liquid Chromatographic Method for the Determination of Hypoxanthine, Xanthine, Uric Acid and Allantoin in Serum

Kock, R.; Delvoux, Bert; Greiling, H.

doi:10.1515/cclm.1993.31.5.303

Cited by 37 publications

(38 citation statements)

References 8 publications

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“…11,[13][14][15] To avoid interferences in the sample matrices, many HPLC methods for the isolation and quantitation of creatinine, uric acid, and other metabolites in biological fluids have been described, including ion-exchange, normal-phase, reversed-phase, and reversed-phase ion-pair chromatography. [16][17][18][19][20][21] Reversed-phase HPLC methods with a highly aqueous mobile phase have been increasingly attractive in light of the recent movement of green analytical chemistry, which encourages the development of analytical methods without or with reduced consumption of organic solvent, energy and time. [17][18][19][22][23][24] However, many studies have reported that the use of a highly aqueous mobile phase (>90% water) with C18 and C8 columns causes an anomalous chromatographic behavior, and a retention loss of analytes.…”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18][19][20][21] Reversed-phase HPLC methods with a highly aqueous mobile phase have been increasingly attractive in light of the recent movement of green analytical chemistry, which encourages the development of analytical methods without or with reduced consumption of organic solvent, energy and time. [17][18][19][22][23][24] However, many studies have reported that the use of a highly aqueous mobile phase (>90% water) with C18 and C8 columns causes an anomalous chromatographic behavior, and a retention loss of analytes. 19,25 These anomalies have been attributed to (i) aggregation of the bonded hydrocarbon chains in the presence of a highly aqueous mobile phase, rendering them inaccessible to analytes, and (ii) extrusion of the highly aqueous mobile phase from the pores of the particles of the stationary phase.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of Creatinine and Uric Acid in Human Urine by High-Performance Liquid Chromatography

et al. 2008

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An environmentally friendly reversed-phase HPLC method for simultaneous determination of creatinine and uric acid in human urine samples has been developed. Human urine samples were pretreated by dilution, protein precipitation, centrifugation and filtration, followed by HPLC separations using a reversed-phase C18 column with an aqueous mobile phase of phosphate buffer. The retention loss of a C18 column when using the highly aqueous mobile phases was avoided by employing a gradient elution using a small volume (<0.23 mL) of acetonitrile and phosphate buffer at pH 4.75. This developed method provides a simple, rapid separation and sensitive detection for the species of interest in 10 min with UV detection at 205 nm. Quantitation was carried out by relating the peak areas of the identified compounds to that of hypoxanthine as an internal standard. The detection limits for creatinine and uric acid were 0.045 and 0.062 mg mL -1 , respectively. The recoveries of the standards added to urine samples were 87.3 -102.2% for creatinine and 97.3 -108.6% for uric acid, and the relative standard deviation for both analytes was less than 1.0%. This method has been successfully applied to estimating of creatinine and uric acid in human urine.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Creatinine and Uric Acid in Human Urine by High-Performance Liquid Chromatography

et al. 2008

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“…Accordingly, it is essential to develop a simple and rapid method for the separation and detection of these bioactive purine bases. Liquid chromatography (LC) [5][6][7][8][9] and capillary electrophoresis (CE) [10][11][12][13] have been widely used for the determination of purine bases and related compounds. To our knowledge, there are no reports on the use of CE microchips for the separation and detection of bioactive purines and related compounds.…”

Section: Introductionmentioning

confidence: 99%

Microchip capillary electrophoresis with a boron-doped diamond electrode for rapid separation and detection of purines

Wang

Chen

Muck

et al. 2004

Journal of Chromatography A

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“…We therefore decided to reduce all ascorbate directly after sampling. Methodological difficulties in analyzing allantoin have resulted in a large number of methodological publications (32)(33)(34), none of which could be applied to our BAL samples.…”

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Antioxidants and Oxidative Stress in BAL Fluid of Atopic Asthmatic Children

et al. 2003

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Earlier studies in adults have indicated that increased oxidative stress may occur in the blood and airways of asthmatic subjects. Therefore the aim of this study was to compare the concentrations of antioxidants and protein carbonyls in bronchoalveolar lavage fluid of clinically stable atopic asthmatic children (AA, n ϭ 78) with our recently published reference intervals for nonasthmatic children (C, n ϭ 124). Additionally, lipid peroxidation products (malondialdehyde) in bronchoalveolar lavage fluid and several antioxidants in plasma were deter- The epithelial lining fluid of the lung with its high concentration of antioxidants provides a first line of defense against inhaled, but also endogenously produced, oxidants (1, 2). Epidemiologic studies have found an inverse association between ascorbate intake and bronchial hyperresponsivness (3). In a small group of patients with exercise-induced asthma, Cohen et al. (4) showed that supplementation with ascorbate improved lung function. Dietary supplementation with a vitamin combination (ascorbic acid, ␣-tocopherol, and ␣-carotene) also had some positive effect on lung function during exercise in combination with ozone exposure (5). Studies in adults living in the United Kingdom have shown that low intake of fresh fruit and vegetables is a risk factor for decreased lung function (6, 7). Changes in ascorbate and urate in the lining fluid of the respiratory tract have been observed as a response to acute air pollution exposure (8) and recently in adults with mild asthma (9). Inflammatory cells from peripheral blood and BAL fluid of asthmatic subjects produce more superoxide anion radicals than those of control subjects (10 -12). Treatment with corticosteroids has been shown to reduce the amount of oxygen radicals released by these cells (13).In children, asthma is defined by symptoms such as wheeze or cough (14), and we have recently shown that atopic asthma is a chronic inflammatory disease in children as in adults (15). Ongoing inflammation in the airways of children with atopic asthma may lead to enhanced oxidative stress in the epithelial lining fluid, which may, as a consequence, lead to a higher consumption of the antioxidants found in BAL fluid. Oxidative damage to BAL proteins may reflect overall oxidative stress to the respiratory tract that could contribute to the underlying pathophysiology of established asthma. We have recently reported concentrations of total ascorbate, urate, ␣-tocopherol, and oxidized proteins in BAL fluid in nonasthmatic children (16

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A High-Performance Liquid Chromatographic Method for the Determination of Hypoxanthine, Xanthine, Uric Acid and Allantoin in Serum

Cited by 37 publications

References 8 publications

Simultaneous Determination of Creatinine and Uric Acid in Human Urine by High-Performance Liquid Chromatography

Simultaneous Determination of Creatinine and Uric Acid in Human Urine by High-Performance Liquid Chromatography

Microchip capillary electrophoresis with a boron-doped diamond electrode for rapid separation and detection of purines

Antioxidants and Oxidative Stress in BAL Fluid of Atopic Asthmatic Children

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