A high-performance liquid-chromatographic method for routine simultaneous determination of nicotine and cotinine in plasma.

Hariharan, M.; VanNoord, Ted; Greden, John F.

doi:10.1093/clinchem/34.4.724

Cited by 151 publications

(36 citation statements)

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“…Cotinine assay was performed in blinded fashion by reversed-phase C-18 ion-pair high performance liquid chromatography (HPLC) and detection by absorbance at 256 nanometers using a modified Hariharan, VanNoord, and Greden ( 1988) protocol. Reported intra-assay coefficients of variations (CVs) were 2.3 to 5.8% and interassay CVs were 2.4 to 5.5%, while extraction efficiency for cotinine standard was 84% (Hariharan et al, 1988). In this study, average within run CV for cotinine assay was 7.6%.…”

Section: Methodsmentioning

confidence: 99%

Factors associated with nicotine dependence among African American women cigarette smokers

Ahijevych

Wewers

1993

Research in Nursing & Health

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Cigarette smoking contributes to disproportionate morbidity and mortality among African Americans. Purposes of the study were to describe smoking behavior and test a model of nicotine dependence among African American women. Participants (n = 187) smoked a low rate of high nicotine mentholated cigarettes and had a mean salivary cotinine of 402 ng/mL. The proposed model predicted 48% of variance in nicotine dependence with smoking to cope, number of cigarettes/day, positive outcome expectancies about smoking, and interest in quitting, as significant contributors. Suggested interventions include developing alternative coping skills, cognitive restructuring, and techniques focused on the precontemplation stage of smoking cessation.

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Section: Methodsmentioning

confidence: 99%

Factors associated with nicotine dependence among African American women cigarette smokers

Ahijevych

Wewers

1993

Research in Nursing & Health

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“…Plasma arterial nicotine and cotinine samples were determined before, 2, 10, and 45-60 min after bolus plus nicotine infusions using the HPLC methods of Hariharan et al (1988).…”

Section: Nicotine Assaymentioning

confidence: 99%

Comparative effects of methamphetamine and nicotine on the striatal [¹¹C]raclopride binding in unanesthetized monkeys

Tsukada¹,

Miyasato²,

Kakiuchi³

et al. 2002

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Although a very large literature exists on the in vitro, ex vivo, and in vivo effects of nicotine on dopamine release in rodents, similar data in primates are scant. This study was initiated to compare methamphetamine to nicotine given i.v. to normal unanesthetized monkeys using positron emission tomography (PET) techniques. Release of dopamine in the striatum using [(11)C]raclopride was determined indirectly in four nicotine-naïve adult Macaca mulatta monkeys under conscious and isoflurane-anesthetized conditions using high-resolution PET. [(11)C]Raclopride was given i.v. as a bolus injection followed by continuous infusion with steady state over 30-45 min. Nicotine bitartrate was then given as a bolus plus infusion for 30 min in doses of 32 microg/kg + 0.8 microg/kg/min or 100 microg/kg + 2.53 microg/kg/min as base. The larger doses of nicotine caused significant cardiovascular effects; these doses did not displace [(11)C]raclopride binding in either dorsal or ventral striatum under the anesthetized conscious condition. In contrast, isoflurane-anesthesia induced a slight but significant dose-dependent reduction of [(11)C]raclopride binding by nicotine even at the same doses used in the anesthetized condition. Methamphetamine in bolus doses of 0.1, 0.3, and 1.0 mg/kg i.v. under conscious condition caused a significant displacement of [(11)C]raclopride and isoflurane-anesthesia facilitated the displacement induced by nicotine. These results indicate that nicotine, in high tobacco-smoking-related doses, does not release sufficient brain dopamine to displace [(11)C]raclopride in the striatum in the awake and fully conscious state, in contrast to small doses of methamphetamine.

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“…We studied 20 normal non-smoking control subjects and 71 asthmatic patients attending the Tsukidate Municipal Hospital. In order to exclude current smokers, we measured urinary cotinine concentration with high-performance liquid chromatography as described by Harlharan et al [12]. We found that urinary cotinine concentrations of all control and asthmatic subjects were less than 30 ng of the cotinine/mg creatinine ratio, the level used to categorize subjects as exposed and unexposed to cigarette smoking [13].…”

Section: Subjectsmentioning

confidence: 99%

Relation between exhaled carbon monoxide levels and clinical severity of asthma

Yamaya

Hosoda

Ishizuka

et al. 2001

Clin Experimental Allergy

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Carbon monoxide (CO) can be detected in exhaled air and is increased in asthmatic patients not treated with corticosteroids. However, it is uncertain whether exhaled CO is related to severity of asthma. To study whether exhaled CO is related to severity of asthma in clinical courses, exhaled CO concentrations were measured on a CO monitor by vital capacity manoeuvre in 20 mild asthmatics treated with inhaled beta2-agonists alone, 20 moderate asthmatics treated with inhaled corticosteroids, and 15 stable asthmatics treated with high dose inhaled corticosteroids and oral corticosteroids once a month over 1 years. Exhaled CO concentrations were also measured in 16 unstable severe asthmatics who visited the hospital every 7 or 14 days for treatment with high dose inhaled corticosteroids and oral corticosteroids. The mean values of exhaled CO in severe asthma over 1 year were 6.7 +/- 9.5 p.p.m. (n = 31, mean +/- SD) and significantly higher than those of non-smoking control subjects (1.2 +/- 0.9 p.p.m., n = 20, P < 0.01). Exhaled CO concentrations in unstable severe asthmatics were significantly higher than those in stable severe asthmatics. However, exhaled CO concentrations in mild and moderate asthmatics did not differ significantly from those in non-smoking control subjects (P > 0.20). There was a significant relationship between the exhaled CO concentrations and forced expiratory volume in one second in all asthmatic patients. These findings suggest that exhaled CO concentrations may relate to the severity of asthma and measurements of exhaled CO concentrations may be a useful means of monitoring airway inflammation in asthma.

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A high-performance liquid-chromatographic method for routine simultaneous determination of nicotine and cotinine in plasma.

Cited by 151 publications

References 0 publications

Factors associated with nicotine dependence among African American women cigarette smokers

Factors associated with nicotine dependence among African American women cigarette smokers

Comparative effects of methamphetamine and nicotine on the striatal [¹¹C]raclopride binding in unanesthetized monkeys

Relation between exhaled carbon monoxide levels and clinical severity of asthma

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A high-performance liquid-chromatographic method for routine simultaneous determination of nicotine and cotinine in plasma.

Cited by 151 publications

References 0 publications

Factors associated with nicotine dependence among African American women cigarette smokers

Factors associated with nicotine dependence among African American women cigarette smokers

Comparative effects of methamphetamine and nicotine on the striatal [11C]raclopride binding in unanesthetized monkeys

Relation between exhaled carbon monoxide levels and clinical severity of asthma

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Comparative effects of methamphetamine and nicotine on the striatal [¹¹C]raclopride binding in unanesthetized monkeys