A high prevalence of mupirocin and macrolide resistance determinant among Staphylococcus aureus strains isolated from burnt patients

Shahsavan, Shadi; Emaneini, Mohammad; Khoshgnab, Behshad Noorazar; Khoramian, Babak; Asadollahi, Parisa; Aligholi, Marzieh; Jabalameli, Fereshteh; Eslampour, Mohammad Amin; Taherikalani, Morovat

doi:10.1016/j.burns.2011.09.004

Cited by 45 publications

(41 citation statements)

References 32 publications

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“…The high level of clindamycin resistance may be associated with inconsistent use of this antibiotic in clinical practice and community and also constitutive and inducible resistance. The rate of resistance to rifampicin was higher in our study (31.2%) compared to those reported in other studies from Germany (2%) (29) and Iran (5%) (24). In contrast to the results of the present study, high level of resistance to rifampicin was reported from Iraqi Kurdistan (57%) (21).…”

Section: Discussioncontrasting

confidence: 94%

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Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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Background: Methicillin resistance Staphylococcus aureus (MRSA) isolates have been recognized as one of the most important causes of infection in burn patients that have recently shown a frequent and rapid development of antibiotic resistance. Objectives: The present study described the distribution of different SCCmec types and antibiotic resistance pattern among MRSA strains isolated from burn patients in a referral burn center in Tehran, Iran. Methods: During a 12-month study, 189 MRSA isolates were obtained from burn patients. Standard culture and biochemical tests were used for identification of MRSA isolates. The antibiotic susceptibility testing was performed using disc diffusion method and the presence of mecA, nucA, pvl, and tst encoding genes was determined using PCR method. In addition, the different SCCmec types were determined using multiplex PCR. Results: All the MRSA isolates were observed to be resistant to amoxicillin and penicillin and sensitive to linezolid, teicoplanin, and vancomycin. The rates of resistance to other antibiotics varied from 86.2% for amikacin to 17.5% for quinupristin-dalfopristin. MDR was observed in all the isolates. Six different antimicrobial resistance patterns were observed among our isolates. Based on the multiplex PCR assay, the two different SCCmec types were detected as 71.4% type III and 28.6% type IV. In total, 8.5% of isolates harbored pvl toxin encoding gene all of which belonging to SCCmec type IV. Furthermore, 33 isolates (17.5%) harbored tst encoding gene. Conclusions:The results showed low diversity of SCCmec type among circulating MRSA in the burn center with relatively high prevalence of SCCmec III. These findings support the need for more studies to elucidate distribution of different SCCmec types among MRSA isolated from burn patients.

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Section: Discussioncontrasting

confidence: 94%

“…The present study showed that mupirocin resistance phenotype was observed in 27% of MRSA isolates. This finding confirmed previous similar observations by Shahsavan et al (24) from Tehran, Iran (25%).…”

Section: Discussionsupporting

confidence: 93%

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Afshari

Sepahi

Goudarzi

et al. 2017

Arch Clin Infect Dis

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“…Shahsavan et al in their inquiry which was performed in 2012, have reported that 58.8% of their S. aureus isolates were resistant to methicillin and 40% were resistant to mupirocin (14). The rate of mupirocin resistance in our study was lower (i.e.…”

Section: Discussioncontrasting

confidence: 59%

Antimicrobial Susceptibility Pattern of Staphylococcus aureus Isolates Against Newly Marketed Antibiotics: A Report From Imam Reza Hospital of Mashhad, Iran

Arianpoor¹,

Estaji²,

Naderinasab³

et al. 2015

Razavi Int J Med

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Background: Infections due to Staphylococcus aureus have long been considered as a big challenge to clinicians. The innate ability of this microorganism to develop resistance to different antibiotics, has led to the appearance of MRSA (methicillin-resistant Staphylococcus aureus) and lately VRSA (vancomycin-resistant Staphylococcus aureus) strains, which are considered as major problems for both patients and clinicians. Objectives: In this study, we tried to evaluate susceptibility pattern of S. aureus isolates against some prevalent antibiotics as well as some infrequent ones. Results: Out of 238 samples, 5.88% were resistant to quinupristin-dalfopristin; 5.46% to linezolid; 60.92% to Co-trimoxazole; 31.93% to doxycycline; 18.90% to tigecycline; 5.04% to vancomycin; 9.24% to mupirocin; 43% to oxacillin and 46.21% of our isolates were resistant to cefoxitin. Conclusions: Coming across isolates with reduced susceptibility to quinupristin-dalfopristin and resistant to linezolid in this study are worrisome although these antibiotics are not used in our hospital. This might be a new challenge in the treatment of MRSA.

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“…In a study conducted by Shahsavan et al (33), it was shown that 25% of mupirucin resistant MRSA isolates carried the mupA gene. Unrestricted policies that allow the improper and widespread use of mupirocin for long periods in hospitals and health care settings and the origin of the isolates and clinical samples are the most important causes of variation in the incidence rate of resistance to mupirocin in MRSA isolates (8,32,33).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Resistance Profile of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospitalized Patients in Intensive Care Unit, Tehran-Iran

Nezhad

Meybodi

Rezaee

et al. 2017

Jundishapur J Microbiol

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Background: Staphylococcus aureus is an important opportunistic pathogen and can cause a wide range of infections. The ability of this pathogen to successfully persist within the hospital and the community is largely due to its remarkable ability to acquire resistance against various antimicrobial agents. Objectives: The aim of this study was to determine the carriage of antibacterial resistance genes and virulence markers of S. aureus isolates from hospitalized patients in intensive care units in Tehran, the capital of Iran. Methods: In this cross-sectional study that was conducted during an 11-month period since March 2015 to January 2016, a total of 105 S. aureus strains were investigated. MRSA screening was performed by phenotypic and genotypic methods. The Kirby-Bauer disk diffusion method was used to assess the sensitivity of S. aureus strains. The strains were typed based on the polymorphisms in SCCmec types. The presence of resistance (ermA, ermB, ermC, mupA, msrA, msrB, tetM, ant (4´)-Ia, aac (6´)-Ie/aph (2˝), aph (3´)-IIIa) and toxin (etb, eta, pvl, tst) encoding genes were investigated by the polymerase chain reaction (PCR) technique. Results: In this study, 105 isolates of S. aureus were obtained from 299 various clinical specimens. Ninety five (90.5%) strains were confirmed as methicillin-resistant S. aureus. The lowest levels of resistance were related to quinupristin-dalfopristin (16.8%) while the highest levels of resistance were related to penicillin (94.7%). Multi-drug resistance was observed in 91.5% of the isolates. Type IV was the most prevalent SCCmec type (57.9%), followed by type III (22.1), type V (12.6%), I (5.3%), and II (2.1%). Overall, 25 isolates (26.3%) harbored PVL-encoding genes, and all of them belonged to SCCmec type IV. The presence of resistance genes ant(4´) ermA, ermB, ermC, msrA, msrB, and tetM was detected in 94.7%, 81.1%, 31.6%, 31.6%, 15.9%, 18.9%, 47.3%, 21.1%, 56.8%. The frequency of the etb, eta, and tst genes were 1.1%, 4.2%, and 32.6%, respectively. Conclusions:The results illustrated the diversity of antibacterial resistance and virulence gene profiles among different SCCmec types of S. aureus. The increased prevalence of methicillin-resistant S. aureus isolates containing different toxin and antibiotic resistance genes is a serious threat for the hospitalized patients in the intensive care units.

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A high prevalence of mupirocin and macrolide resistance determinant among Staphylococcus aureus strains isolated from burnt patients

Cited by 45 publications

References 32 publications

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

Antimicrobial Susceptibility Pattern of Staphylococcus aureus Isolates Against Newly Marketed Antibiotics: A Report From Imam Reza Hospital of Mashhad, Iran

Molecular Characterization and Resistance Profile of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospitalized Patients in Intensive Care Unit, Tehran-Iran

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