A high prevalence of thyroglobulin autoantibodies in adults with and without thyroid disease as measured with a sensitive solid-phase immunosorbent radioassay

Ericsson, Ulla-Britt; Christensen, Svend Borup; Thorell, Jan I.

doi:10.1016/0090-1229(85)90146-1

Cited by 84 publications

(32 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison of different methods for TgAb detection showed that, despite good correlation between the two tests, the immunoassay was more sensitive than the agglutination test, in agreement with the literature (9)(10)(11)(12). The latter test can be negative at low antibody titers, typically < 10IU/mL.…”

Section: Discussionsupporting

confidence: 57%

“…The prevalence of these antibodies in patients with differentiated thyroid carcinoma is relatively high (9)(10)(11)(12), and methods for their detection and information regarding their interference with Tg measurement are therefore important. Interference from TgAb has even been reported for the latest Tg assays (6), and the value of recovery tests to exclude this interference has been questioned (6,9,10,13,14).…”

Section: E S C R I T O R E Smentioning

confidence: 99%

See 1 more Smart Citation

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

Rosário

Maia

Fagundes

et al. 2004

Arq Bras Endocrinol Metab

View full text Add to dashboard Cite

Antithyroglobulin antibodies (TgAb) were measured using a chemiluminescent immunoassay (ICMA) and an agglutination test. TgAb laboratory and clinical interference with Tg measurements were assessed. The course of TgAb concentration and disease status were compared during 3 years after initial treatment. The agglutination test failed to detect all titers < 10IU/mL (ICMA). Interference from TgAb was common at high titers, but even low antibody titers (< 5IU/mL) were able to interfere with Tg measurement. Cases of distant metastases with undetectable Tg (by IRMA) and those apparently free of disease and without thyroid remnants with Tg> 2ng/ml (by RIA) were identified among patients with TgAb. The exogenous Tg recovery test was normal (> 80%) by the two methods in 22% of patients with TgAb and confirmed laboratory interference. Absence of reduction in TgAb levels was a marker of persistent disease. In conclusion, TgAb should be determined by immunoassays; interference with Tg measurements occurred mainly but not always at high concentrations, with a normal Tg recovery test not excluding this interference. The behavior of TgAb is related to disease persistence or cure. Anticorpos anti-tireoglobulina (TgAb) foram medidos por um imunoensaio de quimioluminescência (ICMA) e um teste de aglutinação. Avaliamos a interferência clínica e laboratorial dos TgAb com as medidas de Tg. A evolução da concentração dos TgAb e o estado da doença foram comparados durante 3 anos após o início do tratamento. O teste de aglutinação falhou em detectar todos o títulos <10UI/mL (ICMA). Interferência dos TgAb foi comum com títulos altos, mas mesmo títulos baixos dos anticorpos (<5UI/mL) interferiram na medida de Tg. Casos com metástases à distância e Tg indetectável (por IRMA) e aqueles aparentemente livres da doença e sem remanescentes tireoidenos com Tg >2ng/ml (por RIA) foram identificados entre pacientes com TgAb. O teste de recuperação da Tg exógena foi normal (>80%) por ambos os métodos em 22% dos pacientes com TgAb, confirmando a interferência laboratorial. Ausência de redução dos níveis de TgAb foi um marcador de persistência da doença. Em conclusão, TgAb deve ser determinada por imunoensaios; interferência com as medidas de Tg ocorreram principalmente mas não somente em altas concentrações, com um teste normal de recuperação de Tg não excluindo esta interferência. O comportamento dos TgAb está relacionado à persistência ou à cura da doença.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: E S C R I T O R E Smentioning

confidence: 99%

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

Rosário

Maia

Fagundes

et al. 2004

Arq Bras Endocrinol Metab

View full text Add to dashboard Cite

show abstract

“…We emphasize that anti-thyroid antibodies, anti-Tg and anti-TPO, were measured by fluoroimmunoassay in the present study, yielding a higher sensitivity than that previously reported [21]. This may also have contributed to the higher frequency of AITD observed in the control group.…”

Section: Discussioncontrasting

confidence: 41%

Thyroid disturbance related to chronic hepatitis C infection: role of CXCL10

et al. 2013

View full text Add to dashboard Cite

The associaTion between chronic hepatitis C (HCV) infection and thyroid autoimmune disorders (AITD) is controversial, but may occur or worsen during alpha-interferon treatment [1]. A large study observed slightly increased risk for thyroiditis in HCV-infected patients (hazard ratio = 1.06, 95% CI = 1.01-1.11) [2] and a recent review suggested a weak association in comparisons to hepatitis B (HBV) infected and control populations (odds ratio = 1.6, 95% CI=1.4-1.9). The authors suggested that differences in geographical distribution, genetic variability and environmental cofactors abstract. Association between autoimmune thyroid diseases (AITD) and hepatitis C is controversial, but may occur or worsen during alpha-interferon treatment. The mechanism responsible for autoimmune diseases in infected patients has not been fully elucidated. This study aims to evaluate the frequency of AITD in chronic hepatitis C and the association of chemokine (CXC motif) ligand 10 (CXCL10) and AITD. One hundred and three patients with chronic hepatitis C and 96 controls were prospectively selected to clinical, hormonal, thyroid autoimmunity and ultrasound exams, besides thyroxinebinding globulin (TBG) and CXCL10 measurements and hepatic biopsies. The frequency of AITD among infected subjects was similar to controls. TT3 and TT4 distributions were right shifted, as was TBG, which correlated to both of them. Thyroid heterogeneity and hypoechogenicity were associated with AITD. Increased vascularization was more prevalent in chronic hepatitis C.CXCL10 was higher in infected patients (p=0.007) but was not related to thyroid dysfunction. Increase in CXCL10 levels were consistent with hepatic necroinflammatory activity (p=0.011). In summary, no association was found between chronic hepatitis C and AITD. Infected subjects had higher TT3 and TT4 which were correlated to TBG. Increased CXCL10 was not associated to thyroid dysfunction in HCV-infected population.

show abstract

“…TgAbs are certainly more prevalent in DTC patients than in the general population (4,5 ). Several reports have suggested that patients with AITD have an increased risk of developing thyroid cancer (17,18 ), whereas other reports do not confirm this association (19 ).…”

Section: Discussionmentioning

confidence: 62%

Thyroglobulin Antibodies in Serum of Patients with Differentiated Thyroid Cancer: Relationship between Epitope Specificities and Thyroglobulin Recovery

et al. 2005

View full text Add to dashboard Cite

Background: Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. We identified the epitopes on Tg recognized by TgAb-positive sera from patients with DTC and examined the association between epitope specificity patterns and Tg recovery. Methods: We tested 50 DTC sera for Tg epitope specificity, TgAbs, and Tg recovery. Epitope recognition was determined by use of a panel of 10 well-characterized Tg monoclonal antibodies directed against 6 Tg antigenic clusters (I-VI) in competitive reactions with test sera. Tg was measured by the Thyroglobuline IRMA (CIS bio international). Recovery of added Tg (TgREC) was determined by an in-house assay. Results: Epitope recognition was restricted to immunodominant clusters in 58% of patients, whereas the rest were either broadly heterogeneous (16%) or nonreactive (26%). Median Tg recovery did not differ between sera with restricted and unrestricted specificities (69% vs 80%; P >0.05). TgREC was inversely correlated with the total number of epitopes recognized by sera (r ‫؍‬ ؊0.66; P <0.001). Conclusions: TgAbs with both restricted and broad specificities are present in patients with DTC. TgAb

show abstract

A high prevalence of thyroglobulin autoantibodies in adults with and without thyroid disease as measured with a sensitive solid-phase immunosorbent radioassay

Cited by 84 publications

References 21 publications

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance

Thyroid disturbance related to chronic hepatitis C infection: role of CXCL10

Thyroglobulin Antibodies in Serum of Patients with Differentiated Thyroid Cancer: Relationship between Epitope Specificities and Thyroglobulin Recovery

Contact Info

Product

Resources

About