Currently, canine obesity is a worldwide problem and is based on the excessive deposit of fat tissue, which plays an important role as a store of energy and endocrine organ. This study aimed to compare the values of systolic blood pressure, serum chemistry profile and glucose metabolism in eight obese dogs without endocrine diseases before and after 16 weeks of a weight loss program. A statistical difference was noticed in the serum levels of alkaline phosphatase, gamma glutamyl transferase, glucose, cholesterol and insulin, before and after the program. Besides, there was a statistical difference between the moments in systolic blood pressure, body weight and body condition scale. Therefore, it was concluded that the use of a diet rich in fiber and protein is suitable for performing weight loss and obese dogs undergoing a weight loss program presented a significant reduction in blood pressure, blood glucose, insulin, blood cholesterol, serum activity of alkaline phosphatase and gamma glutamyl transferase.