A high-resolution comparative RH map of the telomeric end of bovine chromosome 2 with human chromosomes 1 and 2

Abstract

High density livestock to human comparative maps are necessary for the implementation of comparative positional candidate gene cloning. We have constructed a high-density comparative radiation hybrid (RH) map of the telomeric end of bovine chromosome 2 (BTA2) using a 12,000-rad whole genome cattle-hamster radiation hybrid (WGRH) panel. Eighteen bovine EST markers with orthologues on human chromosomes 1 and 2 (HSA1 and HSA2), together with nine microsatellite markers, were typed against the 180 cell lines of the WGRH panel. Twenty-one markers were included in the multi-point framework map at LOD =3.0. The comparative analysis reveals a new segment of highly conserved synteny between HSA2 and BTA2.

A gene-based high-resolution comparative radiation hybrid map as a framework for genome sequence assembly of a bovine chromosome 6 region associated with QTL for growth, body composition, and milk performance traits

“…Radiation hybrid (RH) mapping has been shown to be a powerful tool to integrate comparative genome data with information from existing bovine genetic and physical maps to generate high-resolution maps (e.g., [ 6 ]). Considering all RH panels available for the bovine genome, the 12,000 rad whole genome RH panel (12 k WG-RH, [ 7 ]) has been shown to have the high mapping power required to create accurate maps of a highly elevated resolution in defined small chromosomal regions [ 8 - 11 ].…”