A high‐resolution genotyping method for HLA‐C alleles and possible shared HLA‐C‐B haplotypes between Japanese and Caucasians

Wang, H; Tokunaga, Katsushi; Ishikawa, Yoshihide; Tanaka, Hidenori; Kashiwase, Koichi; Shibata, Yoichi; Juji, Takeo

doi:10.1111/j.1399-0039.1997.tb02920.x

Cited by 14 publications

(11 citation statements)

References 11 publications

(11 reference statements)

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“…Most of the associations between alleles of HLA‐A‐Cw‐B loci were also observed in other populations (13–16, 20–22, 24–28). Common Western European haplotypes (A*02‐Cw*07‐B*07, A*02‐Cw*03‐B*15, A*03‐Cw*07‐B*07, A*03‐Cw*04‐B*35, A*29‐Cw*16‐B*44, and A*33‐Cw*08‐B*14) (13) were found at similar frequencies in the Pernambuco population (0.0159, 0.0198, 0.0109, 0.0149, and 0.0149, respectively), and haplotypes observed at lower frequency in Western Europe such as A*01‐Cw*07‐B*08 and A*24‐Cw*04‐B*35 (13) were also found at similar frequencies in the population of this study (both with a frequency of 0.0099).…”

Section: Hla Typingmentioning

confidence: 54%

Polymorphism of HLA class I genes in the Brazilian population from the Northeastern State of Pernambuco corroborates anthropological evidence of its origin

et al. 2004

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The allelic distribution of human leukocyte antigen (HLA) class I genes (HLA-A, HLA-B, and HLA-Cw) of the population from the State of Pernambuco in Northeastern Brazil was studied in a sample of 101 healthy unrelated individuals. Low to medium resolution HLA class I typing was performed using polymerase chain reaction-amplified DNA hybridized to sequence specific primers (PCR-SSPs). Twenty allele groups were detected for HLA-A, 28 for HLA-B, and 14 for HLA-Cw. The most frequent alleles were HLA-A*02(0.2871), HLA-B*15(0.1238), and HLA-Cw*04(0.2277), and the most frequent genotypes were A*02/A*02(0.0990), B*15/B*15(0.0594), and Cw*04/Cw*04 and Cw*07/Cw*07, both with a frequency of 0.0792. The observed heterozygosity for the studied loci was 79.21% for HLA-A, 87.13% for HLA-B, and 77.23% for HLA-Cw. The most frequent haplotype was A*02-Cw*04-B*35(0.0485), which is also present in Western European, Amerindian, and Brazilian Mulatto populations, but absent in African populations. Taken together, these data corroborate the historic anthropological evidences of the origin of the Northeastern Brazilian population from Pernambuco.

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Section: Hla Typingmentioning

confidence: 54%

Polymorphism of HLA class I genes in the Brazilian population from the Northeastern State of Pernambuco corroborates anthropological evidence of its origin

et al. 2004

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“…KIR genotype AA and HLA-C group 2 frequencies in different populations. Most of the HLA-C and KIR data have been taken from different cohorts within each population (references[26][27][28][29][30][31][32][33][34][35]. The correlation coefficient (r) was Ϫ0.82.…”

mentioning

confidence: 99%

Combinations of Maternal KIR and Fetal HLA-C Genes Influence the Risk of Preeclampsia and Reproductive Success

Hiby

Walker

O’Shaughnessy

et al. 2004

The Journal of Experimental Medicine

999

860

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Preeclampsia is a serious complication of pregnancy in which the fetus receives an inadequate supply of blood due to failure of trophoblast invasion. There is evidence that the condition has an immunological basis. The only known polymorphic histocompatibility antigens on the fetal trophoblast are HLA-C molecules. We tested the idea that recognition of these molecules by killer immunoglobulin receptors (KIRs) on maternal decidual NK cells is a key factor in the development of preeclampsia. Striking differences were observed when these polymorphic ligand: receptor pairs were considered in combination. Mothers lacking most or all activating KIR (AA genotype) when the fetus possessed HLA-C belonging to the HLA-C2 group were at a greatly increased risk of preeclampsia. This was true even if the mother herself also had HLA-C2, indicating that neither nonself nor missing-self discrimination was operative. Thus, this interaction between maternal KIR and trophoblast appears not to have an immune function, but instead plays a physiological role related to placental development. Different human populations have a reciprocal relationship between AA frequency and HLA-C2 frequency, suggesting selection against this combination. In light of our findings, reproductive success may have been a factor in the evolution and maintenance of human HLA-C and KIR polymorphisms.

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“…High resolution typing of HLA-C has been performed by PCR-SP (8,9), PCR-SCP in combination with S P (20), PCR-SO (5, 11) and allele separation followed by PCR-SO (21). The major drawback of these methods is the rapidly increasing number of HLA-C alleles identified, and hence the increasing number of primers and probes needed for high resolution typing.…”

Section: Discussionmentioning

confidence: 99%

A reliable and efficient high resolution typing method for HLA‐C using sequence‐based typing

1998

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Serological typing of HLA-C has been poor and almost half of its alleles are serologically undetectable blanks in most populations. Therefore, DNA typing techniques have been used to identify and type for the HLA-C gene. Sequence-based typing (SBT) has proven a major typing strategy for highly polymorphic HLA genes. The technique enables direct identification of all sequence motifs without the need to continuously adjust primers. Here we describe a reliable solid-phase SBT strategy for HLA-C which can be used to distinguish all currently known HLA-C alleles without prior knowledge gained by low resolution typing. Exons 2 and 3 were amplified and sequenced and if necessary sequences of exons 1 and 5 were determined. A total of 257 individuals were typed for HLA-C using this protocol and 30 of the 42 known HLA-C alleles were detected. All heterozygous combinations found in this study were unambiguously discriminated. One hundred and forty-four individuals from the Dutch population were typed randomly. In this group Cw*0701 and *0702 were the most frequently detected alleles. Of the serological Cw blank alleles Cw*1203 was found to have the highest frequency (16%). From the total group 212 individuals were typed serologically and 106 were retyped with 97 selected antisera to further compare serological and molecular defined phenotypes. Discrepancies between serological typing and SBT are mainly attributable to the serologically Cw blank alleles Cw*12-18. The high resolution SBT protocol described will be a valuable tool for the identification of HLA-C alleles and the determination of the role of HLA-C in marrow and organ transplantation.

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A high‐resolution genotyping method for HLA‐C alleles and possible shared HLA‐C‐B haplotypes between Japanese and Caucasians

Cited by 14 publications

References 11 publications

Polymorphism of HLA class I genes in the Brazilian population from the Northeastern State of Pernambuco corroborates anthropological evidence of its origin

Polymorphism of HLA class I genes in the Brazilian population from the Northeastern State of Pernambuco corroborates anthropological evidence of its origin

Combinations of Maternal KIR and Fetal HLA-C Genes Influence the Risk of Preeclampsia and Reproductive Success

A reliable and efficient high resolution typing method for HLA‐C using sequence‐based typing

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