A high sensitive microwave sensor to monitor bacterial and biofilm growth

Longo, Matthieu; Rioual, Stéphane; Talbot, Philippe; Faÿ, Fabienne; Hellio, Claire; Lescop, Benoît

doi:10.1016/j.sbsr.2022.100493

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…The black curve corresponds to the control. The S11 coefficient decreases with time and draws two phases corresponding to planktonic growth and biofilm formation as observed in previous experiments with this sensor [22]. This result was expected since the experiment is identical to the previous one, except for the nature of the culture medium [22].…”

Section: Bacterial Development Monitoringsupporting

confidence: 82%

“…In this study, a customized radio frequency sensor derived from the one presented in our previous work [22] was used to investigate the electric properties of biosolutions and run growth kinetics. It is made of a dielectric line wrapped with an insulating layer (PTFE), shown in Figure 1.…”

Section: Radiofrequency Characterizationmentioning

confidence: 99%

“…Our current study builds on our previous work [22], where we developed a sensor for real-time bacterial growth monitoring based on radio frequency measurements of biological electrical properties. This paper focuses on the potential applications of the biofilm sensor in monitoring biofilm and bacterial growth, evaluating its effectiveness compared to traditional optical density measurements and microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

Longo,

Lelchat,

Le Baut

et al. 2024

Sensors

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Confronting the challenge of biofilm resistance and widespread antimicrobial resistance (AMR), this study emphasizes the need for innovative monitoring methods and explores the potential of bacteriophages against bacterial biofilms. Traditional methods, like optical density (OD) measurements and confocal microscopy, crucial in studying biofilm–virus interactions, often lack real-time monitoring and early detection capabilities, especially for biofilm formation and low bacterial concentrations. Addressing these gaps, we developed a new real-time, label-free radiofrequency sensor for monitoring bacteria and biofilm growth. The sensor, an open-ended coaxial probe, offers enhanced monitoring of bacterial development stages. Tested on a biological model of bacteria and bacteriophages, our results indicate the limitations of traditional OD measurements, influenced by factors like sedimented cell fragments and biofilm formation on well walls. While confocal microscopy provides detailed 3D biofilm architecture, its real-time monitoring application is limited. Our novel approach using radio frequency measurements (300 MHz) overcomes these shortcomings. It facilitates a finer analysis of the dynamic interaction between bacterial populations and phages, detecting real-time subtle changes. This method reveals distinct phases and breakpoints in biofilm formation and virion interaction not captured by conventional techniques. This study underscores the sensor’s potential in detecting irregular viral activity and assessing the efficacy of anti-biofilm treatments, contributing significantly to the understanding of biofilm dynamics. This research is vital in developing effective monitoring tools, guiding therapeutic strategies, and combating AMR.

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Section: Bacterial Development Monitoringsupporting

confidence: 82%

Section: Radiofrequency Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tracking of Bacteriophage Predation on Pseudomonas aeruginosa Using a New Radiofrequency Biofilm Sensor

Longo,

Lelchat,

Le Baut

et al. 2024

Sensors

Self Cite

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“…In some instances, such as the work performed by Revil et al [ 64 ] on brine-saturated clayey soils, the word “induced polarization” is used as an alternative name for complex conductivity, which is just an alternative name for AC impedance spectroscopy. Longo et al [ 34 ] developed an impedance-based sensor applied at the microwave range to detect the initiation of biofilm in cases where low detection levels are desired. The sensor used an open-ended coaxial probe in the microwave range, which was shown to be highly sensitive in the early stages of biofilm growth, using Vibrio natriegens and P. aeruginosa .…”

Section: Review Of Potential Methods For Sensing Biofilm Activity In ...mentioning

confidence: 99%

“…Depending on the physical, chemical, and biological conditions of the environment of interest, methods of studying biofilm differ from one another in the sense that the monitoring method used in one environment or application may not be as useful when utilized in a different application [ 33 ]. Despite the research and availability of biosensors allowing for the real-time monitoring proposed in the literature [ 34 ], monitoring biofilm in real-time and in situ remains a challenge [ 35 ]. Rodriguez-Mozaz et al [ 36 ] assert that in spite of past improvements in biosensor technology, there still exists the challenge of developing a more reliable device.…”

Section: Introductionmentioning

confidence: 99%

Sensors for Biomass Monitoring in Vegetated Green Infrastructure: A Review

Jalilian

Valeo

Chu

et al. 2023

Sensors

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Bioretention cells, or rain gardens, can effectively reduce many contaminants in polluted stormwater through phytoremediation and bioremediation. The vegetated soil structure develops bacterial communities both within the soil and around the vegetation roots that play a significant role in the bioremediative process. Prediction of a bioretention cell’s performance and efficacy is essential to the design process, operation, and maintenance throughout the design life of the cell. One of the key hurdles to these important issues and, therefore, to appropriate designs, is the lack of effective and inexpensive devices for monitoring and quantitatively assessing this bioremediative process in the field. This research reviews the available technologies for biomass monitoring and assesses their potential for quantifying bioremediative processes in rain gardens. The methods are discussed based on accuracy and calibration requirements, potential for use in situ, in real-time, and for characterizing biofilm formation in media that undergoes large fluctuations in nutrient supply. The methods discussed are microscopical, piezoelectric, fiber-optic, thermometric, and electrochemical. Microscopical methods are precluded from field use but would be essential to the calibration and verification of any field-based sensor. Piezoelectric, fiber-optic, thermometric, and some of the electrochemical-based methods reviewed come with limitations by way of support mechanisms or insufficient detection limits. The impedance-based electrochemical method shows the most promise for applications in rain gardens, and it is supported by microscopical methods for calibration and validation.

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