2019
DOI: 10.1002/cyto.b.21831
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A High‐Sensitivity 10‐Color Flow Cytometric Minimal Residual Disease Assay in B‐Lymphoblastic Leukemia/Lymphoma Can Easily Achieve the Sensitivity of 2‐in‐106and Is Superior to Standard Minimal Residual Disease Assay: A Study of 622 Patients

Abstract: BackgroundFlow‐cytometric minimal residual disease (FC‐MRD) monitoring is a well‐established risk‐stratification factor in B‐lymphoblastic leukemia/lymphoma (‐B‐ALL) and is being considered as a basis for deintensification or escalation in treatment protocols. However, currently practiced standard FC‐MRD has limited sensitivity (up to 0.01%) and higher false MRD‐negative rate. Hence, a highly sensitive, widely applicable, and easily reproducible FC‐MRD assay is needed, which can provide a reliable basis for th… Show more

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Cited by 59 publications
(97 citation statements)
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“…MFC-MRD was performed in BM aspirate samples at an early time-point i.e., at the end of induction (post-induction, PI; day 35-40) and at a later time-point i.e., the post-consolidation (PC-MRD, day 78-83). BM samples for MRD assessment were processed using Euroflow bulk-lysis protocol (20) and an 10-color antibody-panel. In brief, the cell suspension was prepared by bulk erythrocyte lysing with ammonium chloridebased lysing reagent, and the cells were stained with the 11-antibody 10-color MRD panel (Supplementary Table S2).…”
Section: Mfc Mrd Monitoringmentioning
confidence: 99%
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“…MFC-MRD was performed in BM aspirate samples at an early time-point i.e., at the end of induction (post-induction, PI; day 35-40) and at a later time-point i.e., the post-consolidation (PC-MRD, day 78-83). BM samples for MRD assessment were processed using Euroflow bulk-lysis protocol (20) and an 10-color antibody-panel. In brief, the cell suspension was prepared by bulk erythrocyte lysing with ammonium chloridebased lysing reagent, and the cells were stained with the 11-antibody 10-color MRD panel (Supplementary Table S2).…”
Section: Mfc Mrd Monitoringmentioning
confidence: 99%
“…(9,(17)(18)(19). On the contrary, MFC-MRD has advantages like wider availability, easy processing, and is relatively inexpensive (9,19,20). Most importantly, it has a very low turn-around-time making it suitable for quick therapeutic decisions (20).…”
Section: Introductionmentioning
confidence: 99%
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“…However, things have changed, as lossless compression algorithms (LCAs) nowadays can achieve significant space savings, without severely impacting user experience. On the other hand, long‐term storage remains fairly expensive, and data sets keep growing in size, in terms of parameters and events .…”
mentioning
confidence: 99%
“…MRD phenotypes will show markers similar to relapsed leukemia cells saved for reference from original diagnostic cells or can be identified through profile databases in the case of secondary ALL. In contrast to the four-color flow cytometers, which have the capacity to measure MRD up to the important diagnostic level of 0.01%, 8-12 color flow cytometers can normally measure MRD levels up to 0.001% cells or 1 MRD cell in 100,000 cells in a bone marrow specimen with even higher sensitivity [43,53].…”
Section: Use Of Patient-specific Reagentmentioning
confidence: 99%