A high-sensitivity cfDNA capture enables to detect the BRAF V600E mutation in papillary thyroid carcinoma

Lee, Tae Hee; Jeon, Hyeon‐Gu; Choi, Jung Hyun; Kim, Young Jun; Hwangbo, Pil-Neo; Park, Hyun Sung; Son, Chae Yeon; Choi, Hei-Gwon; Kim, Ha Neul; Chang, Jae Won; Bu, Jong‐Uk; Eun, Hyuk Soo

doi:10.1007/s11814-022-1348-0

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Therefore, the specific direction of the research has been tuned to find a disease-related sequence in cfDNAs. Key mutations like rat viral sarcoma ( RAS ), EGFR , PIK356 , BRAF , and TP53 were targeted to estimate the actual fraction of ctDNA from total cfDNA [ 156 – 158 ]. The representative studies using optical nanomaterials are described in Table 6 .…”

Section: Optical Nanomaterial-based Detection Of Lb Biomarkersmentioning

confidence: 99%

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Kim,

Rho,

Park

et al. 2024

J Hematol Oncol

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Liquid biopsy, which is a minimally invasive procedure as an alternative to tissue biopsy, has been introduced as a new diagnostic/prognostic measure. By screening disease-related markers from the blood or other biofluids, it promises early diagnosis, timely prognostication, and effective treatment of the diseases. However, there will be a long way until its realization due to its conceptual and practical challenges. The biomarkers detected by liquid biopsy, such as circulating tumor cell (CTC) and circulating tumor DNA (ctDNA), are extraordinarily rare and often obscured by an abundance of normal cellular components, necessitating ultra-sensitive and accurate detection methods for the advancement of liquid biopsy techniques. Optical biosensors based on nanomaterials open an important opportunity in liquid biopsy because of their enhanced sensing performance with simple and practical properties. In this review article, we summarized recent innovations in optical nanomaterials to demonstrate the sensitive detection of protein, peptide, ctDNA, miRNA, exosome, and CTCs. Each study prepares the optical nanomaterials with a tailored design to enhance the sensing performance and to meet the requirements of each biomarker. The unique optical characteristics of metallic nanoparticles (NPs), quantum dots, upconversion NPs, silica NPs, polymeric NPs, and carbon nanomaterials are exploited for sensitive detection mechanisms. These recent advances in liquid biopsy using optical nanomaterials give us an opportunity to overcome challenging issues and provide a resource for understanding the unknown characteristics of the biomarkers as well as the mechanism of the disease. Graphical abstract

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Section: Optical Nanomaterial-based Detection Of Lb Biomarkersmentioning

confidence: 99%

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Kim,

Rho,

Park

et al. 2024

J Hematol Oncol

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“…The bead was carefully dipped into 350 µL buffer AW1 (Qiagen, Hilden, Germany) for 60 s, then dipped into 50 µL Rnase/Dnase free water for 3 min. The elution was stored at −80 • C. DNA was extracted using a QIAamp DNA Mini kit (Qiagen) following the instructions provided by the manufacturer as described previously [21,22].…”

Section: Buffy Coat Separationmentioning

confidence: 99%

Programmed Death 1 and Cytotoxic T-Lymphocyte-Associated Protein 4 Gene Expression in Peripheral Blood Mononuclear Cells Can Serve as Prognostic Biomarkers for Hepatocellular Carcinoma

Lee,

Choi,

Eun

et al. 2024

Cancers

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Hepatocellular carcinoma (HCC) is a highly aggressive form of liver cancer with poor prognosis. The lack of reliable biomarkers for early detection and accurate diagnosis and prognosis poses a significant challenge to its effective clinical management. In this study, we investigated the diagnostic and prognostic potential of programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) expression in peripheral blood mononuclear cells (PBMCs) in HCC. PD-1 and CTLA-4 gene expression was analyzed comparatively using PBMCs collected from HCC patients and healthy individuals. The results revealed higher PD-1 gene expression levels in patients with multifocal tumors, lymphatic invasion, or distant metastasis than those in their control counterparts. However, conventional serum biomarkers of liver function do not exhibit similar correlations. In conclusion, PD-1 gene expression is associated with OS and PFS and CTLA-4 gene expression is associated with OS, whereas the serum biomarkers analyzed in this study show no significant correlation with survival in HCC. Hence, PD-1 and CTLA-4 expressed in PBMCs are considered potential prognostic biomarkers for patients with HCC that can facilitate prediction of malignancy, response to currently available HCC treatments, and overall survival.

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Polysaccharide Hydrogel-Assisted Biosensing Platforms for Point-of-Care Use

Kim,

Lee

2025

Biosensors

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Point-of-care (POC) use is one of the essential goals of biosensing platforms. Because the increasing demand for testing cannot be met by a centralized laboratory-based strategy, rapid and frequent testing at the right time and place will be key to increasing health and safety. To date, however, there are still difficulties in developing a simple and affordable, as well as sensitive and effective, platform that enables POC use. In terms of materials, hydrogels, a unique family of water-absorbing biocompatible polymers, have emerged as promising components for the development of biosensors. Combinations of hydrogels have various additional applications, such as in hydrophilic coatings, nanoscale filtration, stimuli-responsive materials, signal enhancement, and biodegradation. In this review, we highlight the recent efforts to develop hydrogel-assisted biosensing platforms for POC use, especially focusing on polysaccharide hydrogels like agarose, alginate, chitosan, and so on. We first discuss the pros and cons of polysaccharide hydrogels in practical applications and then introduce case studies that test different formats, such as paper-based analytical devices (PADs), microfluidic devices, and independent platforms. We believe the analysis in the present review provides essential information for the development of biosensing platforms for POC use in resource-limited settings.

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A high-sensitivity cfDNA capture enables to detect the BRAF V600E mutation in papillary thyroid carcinoma

Cited by 3 publications

References 26 publications

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Programmed Death 1 and Cytotoxic T-Lymphocyte-Associated Protein 4 Gene Expression in Peripheral Blood Mononuclear Cells Can Serve as Prognostic Biomarkers for Hepatocellular Carcinoma

Polysaccharide Hydrogel-Assisted Biosensing Platforms for Point-of-Care Use

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