A High-Throughput Arabidopsis Reverse Genetics System

Sessions, Allen; Burke, Ellen; Presting, Gernot G.; Aux, George; McElver, John; Patton, David A.; Dietrich, Bob; Ho, Patrick; Bacwaden, Johana; Ko, Cynthia; Clarke, Joseph D.; Cotton, David; Bullis, David; Snell, Jennifer; Miguel, Trini; Hutchison, Don; Kimmerly, Bill; Mitzel, Theresa; Katagiri, Fumiaki; Glazebrook, Jane; Law, Marc; Goff, Stephen A.

doi:10.1105/tpc.004630

Cited by 868 publications

(835 citation statements)

References 35 publications

Supporting

Mentioning

818

Contrasting

Unclassified

Order By: Relevance

“…The mutants used were rpt2a-2 (SALK 005596), rpt2b-1 (SALK 043450), rpt5a-4 (SALK 046321), 17) and rpt5b-3 (SAIL 293 H08). 18) Lines carrying T-DNA in the homozygote were established, and the presence of T-DNA was determined by PCR using the following primer sets: T-DNA specific primers LBb1 5 0 -GCGTGGACCGCTTGCTGCAACT-3 0 and 5 0 -GGAATTGTTTTACCGGAGGAG-3 0 for rpt2a-2; 5 0 -ACCGC-AAAGTAAAAATGATGG-3 0 for rpt2b-1; 5 0 -ATGATGCTGAGGAA-GATGGTG-3 0 for rpt5a-4; and 5 0 -AACCACACTTTGAAGGGGTTC-3 0 for rpt5b-3.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis thaliana26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance

Sakamoto

Kamiya

Sako

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Arabidopsis thaliana26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance

Sakamoto

Kamiya

Sako

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…These lines are homozygous F 2 progeny from crosses of insertion lines SLAT_15-21 (Atmlo5-2) and GARLIC_348_B04 (Atmlo9-2), SALK_118934 (Atmlo5-5) and SALK_073198 (Atmlo9-3), as well as SLAT_24-28 (Atmlo5-1) and GARLIC_348_B04 (Atmlo9-2), respectively. Sequence-indexed SALK [18] and GARLIC (now referred to as SAIL) [19] T-DNA insertion lines were obtained from the Nottingham Arabidopsis Stock Centre (NASC; http://arabidopsis.info/) and Syngenta Biotechnology, respectively. SLAT transposon insertion lines [20] were selected by PCR using pooled DNA samples and appropriate oligonucleotides.…”

Section: Methodsmentioning

confidence: 99%

A reference map of the Arabidopsis thaliana mature pollen proteome

Noir

Bräutigam

Colby

et al. 2005

Biochemical and Biophysical Research Communications

137

145

View full text Add to dashboard Cite

The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome. Ó 2005 Elsevier Inc. All rights reserved.Keywords: Arabidopsis thaliana; Male gametophyte; Mass spectrometry; Mature pollen; Proteome; Two-dimensional gel electrophoresis In higher plants, development of the male gametophyte is a well-programmed and elaborate process. Male sporogenesis begins with the division of a diploid sporophytic cell giving rise to the anther wall of the stamen and the sporogenic cells. The latter cells then undergo several mitoses to differentiate into pollen mother cells. Subsequently, each diploid pollen mother cell forms a tetrad of haploid microspores via a round of DNA replication followed by two consecutive meiotic divisions. Each uninucleate microspore then undergoes an asymmetric mitotic division forming a large vegetative cell and a smaller generative cell (i.e., the bicellular pollen stage). In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction. In addition, it represents a very useful model for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth [5][6][7].The availability of the full genome sequence of Arabidopsis [8] has made genome-wide, microarray-based analyses of the male gametophyte transcriptome of this model plant possible [9][10][11][12]. 13,977 male gametophyte-expressed mRNAs were recently identified using the ATH1 Genome Array, which cover...

show abstract

“…The Col-0 mutant npr1-1 and the Ws-0 transgenic Arabidopsis NahG line were kindly provided by X. Dong (Duke University, Durham, NC) and F. Mauch (University of Fribourg, Switzerland), respectively. The IBS1 T-DNA insertion lines SAIL_634_B09 (s-634), SAIL_111_B09 (s-111), and SAIL_765_A05 (s-765) were obtained from the Arabidopsis T-DNA insertion collection of Syngenta (Research Triangle Park, NC) (Sessions et al, 2002). The IBS2 T-DNA insertion line SALK_031243 (s-031243) was obtained from the Arabidopsis T-DNA insertion collection of the Salk Institute (Alonso et al, 2003).…”

Section: Plant Linesmentioning

confidence: 99%

Dissecting the β-Aminobutyric Acid–Induced Priming Phenomenon in Arabidopsis

et al. 2005

View full text Add to dashboard Cite

Plants treated with the nonprotein amino acid β-aminobutyric acid (BABA) develop an enhanced capacity to resist biotic and abiotic stresses. This BABA-induced resistance (BABA-IR) is associated with an augmented capacity to express basal defense responses, a phenomenon known as priming. Based on the observation that high amounts of BABA induce sterility in Arabidopsis thaliana, a mutagenesis screen was performed to select mutants impaired in BABA-induced sterility (ibs). Here, we report the isolation and subsequent characterization of three T-DNA–tagged ibs mutants. Mutant ibs1 is affected in a cyclin-dependent kinase–like protein, and ibs2 is defective in AtSAC1b encoding a polyphosphoinositide phosphatase. Mutant ibs3 is affected in the regulation of the ABA1 gene encoding the abscisic acid (ABA) biosynthetic enzyme zeaxanthin epoxidase. To elucidate the function of the three IBS genes in plant resistance, the mutants were tested for BABA-IR against the bacterium Pseudomonas syringae pv tomato, the oomycete Hyaloperonospora parasitica, and BABA-induced tolerance to salt. All three ibs mutants were compromised in BABA-IR against H. parasitica, although to a different extent. Whereas ibs1 was reduced in priming for salicylate (SA)-dependent trailing necrosis, mutants ibs2 and ibs3 were affected in the priming for callose deposition. Only ibs1 failed to express BABA-IR against P. syringae, which coincided with a defect in priming for SA-inducible PR-1 gene expression. By contrast, ibs2 and ibs3 showed reduced BABA-induced tolerance to salt, which correlated with an affected priming for ABA-inducible gene expression. For all three ibs alleles, the defects in BABA-induced sterility and BABA-induced protection against P. syringae, H. parasitica, and salt could be confirmed in independent mutants. The data presented here introduce three novel regulatory genes involved in priming for different defense responses.

show abstract

A High-Throughput Arabidopsis Reverse Genetics System

Cited by 868 publications

References 35 publications

Arabidopsis thaliana26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance

Arabidopsis thaliana26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance

A reference map of the Arabidopsis thaliana mature pollen proteome

Dissecting the β-Aminobutyric Acid–Induced Priming Phenomenon in Arabidopsis

Contact Info

Product

Resources

About