A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset

Lin, Mo Jun; Takahashi, Yeonsook S.; Huo, Bing-Xing; Hanada, Mitsutoshi; Nagashima, Jaimi; Hata, Junichi; Tolpygo, Alexander; Ram, Keerthi; Lee, Brian C.; Miller, Michael I.; Rosa, Marcello G. P.; Sasaki, Erika; Iriki, Atsushi; Okano, Hideyuki; Mitra, Partha P.

doi:10.7554/elife.40042

Cited by 65 publications

(49 citation statements)

References 74 publications

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“…The Nissl and myelin series were scanned with bright field illumination, and the fluorescence series with a tri-pass filter cube (FITC/TX-RED/DAPI) for excitation. All imaging data were processed with a high-throughput neurohistological pipeline (Lin et al 2018), resulting in high resolution images (e.g. Fig.…”

Section: Tissue Processing and Data Analysis (Experiments Performed Amentioning

confidence: 99%

Unidirectional monosynaptic connections from auditory areas to the primary visual cortex in the marmoset monkey

Rosa

Majka²,

Bai

et al. 2018

Preprint

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Until the late 20 th Century, it was believed that different sensory modalities were processed by largely independent pathways in the primate cortex, with cross-modal integration only occurring in specialized polysensory areas. This model was challenged by the finding that the peripheral representation of the primary visual cortex (V1) receives monosynaptic connections from areas of the auditory cortex in the macaque. However, auditory projections to V1 have not been reported in other primates. We investigated the existence of direct interconnections between V1 and auditory areas in the marmoset, a New World monkey.Labelled neurons in auditory cortex were observed following 4 out of 10 retrograde tracer injections involving V1. These projections to V1 originated in the caudal subdivisions of auditory cortex (primary auditory cortex, caudal belt and parabelt areas), and targeted parts of V1 that represent parafoveal and peripheral vision. Injections near the representation of the vertical meridian of the visual field labelled few or no cells in auditory cortex. We also placed 8 retrograde tracer injections involving core, belt and parabelt auditory areas, none of which revealed direct projections from V1. These results confirm the existence of a direct, nonreciprocal projection from auditory areas to V1 in a different primate species, which has evolved separately from the macaque for over 30 million years. The essential similarity of these observations between marmoset and macaque indicate that early-stage audiovisual integration is a shared characteristic of primate sensory processing.

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Section: Tissue Processing and Data Analysis (Experiments Performed Amentioning

confidence: 99%

Unidirectional monosynaptic connections from auditory areas to the primary visual cortex in the marmoset monkey

Rosa

Majka²,

Bai

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, eFLASH can facilitate studies of animal models with limited access to genetic labeling methodologies. The Common Marmoset is an emerging primate model for social behaviors with many experimental advantages 51,52 , and thus much effort has been undertaken to construct marmoset brain atlases in diverse modalities [53][54][55] . Unfortunately, numerous hurdles remain in translating existing genetic labeling approaches for rodents to marmosets.…”

Section: Discussionmentioning

confidence: 99%

Ultrafast immunostaining of organ-scale tissues for scalable proteomic phenotyping

Yun

Park

Cho

et al. 2019

Preprint

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Studying the function and dysfunction of complex biological systems necessitates comprehensive understanding of individual cells. Advancements in three-dimensional (3D) tissue processing and imaging modalities have enabled rapid visualization and phenotyping of cells in their spatial context. However, system-wide interrogation of individual cells within large intact tissue remains challenging, low throughput, and error-prone owing to the lack of robust labeling technologies.Here we introduce a rapid, versatile, and scalable method, eFLASH, that enables complete and uniform labeling of organ-scale tissue within one day. eFLASH dynamically modulates chemical transport and reaction kinetics to establish system-wide uniform labeling conditions throughout the day-long labeling period. This unique approach enables the same protocol to be compatible with a wide range of tissue types and probes, enabling combinatorial molecular phenotyping across different organs and species. We applied eFLASH to generate quantitative maps of various cell types in mouse brains. We also demonstrated multidimensional cell profiling in a marmoset brain block. We envision that eFLASH will spur holistic phenotyping of emerging animal models and disease models to help assess their functions and dysfunctions.

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“…This method enables a high-throughput preparation of histological slices with reduced sectioning-induced damages, and the authors report a preparation time of 4 h to slice a single frozen block into 20 microns thick histological slides. This system was used to obtain a brain-wide mesoscale connectivity mapping of the common marmoset [135]. The reported time to prepare and image a full Nissl marmoset brain was 6.4 days.…”

Section: Alternatives To Sbhmentioning

confidence: 99%

A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging

2019

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In recent years, multiple serial histology techniques were developed to enable whole rodent brain imaging in 3-D. The main driving forces behind the emergence of these imaging techniques were the genome-wide atlas of gene expression in the mouse brain, the pursuit of the mouse brain connectome, and the BigBrain project. These projects rely on the use of optical imaging to target neuronal structures with histological stains or fluorescent dyes that are either expressed by transgenic mice or injected at specific locations in the brain. Efforts to adapt the serial histology acquisition scheme to use intrinsic contrast imaging (ICI) were also put forward, thus leveraging the natural contrast of neuronal tissue. This review focuses on these efforts. First, the origin of optical contrast in brain tissue is discussed with emphasis on the various imaging modalities exploiting these contrast mechanisms. Serial blockface histology (SBH) systems using ICI modalities are then reported, followed by a review of some of their applications. These include validation studies and the creation of multimodal brain atlases at a micrometer resolution. The paper concludes with a perspective of future developments, calling for a consolidation of the SBH research and development efforts around the world. The goal would be to offer the neuroscience community a single standardized open-source SBH solution, including optical design, acquisition automation, reconstruction algorithms, and analysis pipelines.

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A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset

Cited by 65 publications

References 74 publications

Unidirectional monosynaptic connections from auditory areas to the primary visual cortex in the marmoset monkey

Unidirectional monosynaptic connections from auditory areas to the primary visual cortex in the marmoset monkey

Ultrafast immunostaining of organ-scale tissues for scalable proteomic phenotyping

A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging

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