A high γ-aminobutyric acid-producingLactobacillus brevis isolated from Chinese traditionalpaocai

Li, Haixing; Gao, Dandan; Cao, Yusheng; Xu, Hengyi

doi:10.1007/bf03175570

Cited by 86 publications

(66 citation statements)

References 23 publications

(32 reference statements)

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“…The levels of GABA that can be achieved in vitro by probiotic organisms are quite large. For example, in the production of fermented foodstuffs, such as Japanese funa-sushi and Chinese traditional paocai, which employ lactobacilli as part of the manufacturing processing, GABA levels in the millimolar range have been demonstrated in the final products [28][29][30]. Secondly, as the predominant inhibitory neurotransmitter in the nervous system, GABA also serves a receptor-mediated role in a number of immunological (i.e.…”

Section: Testing the Hypothesismentioning

confidence: 98%

“…The in vitro identification of sets of high and non-GABA producing probiotics could be performed using standard growth methodology combined with analysis of GABA production in place of the more involved generation of mutants by sitedirected mutagenesis. The feasibility of finding high and non-GABA producing isolates has already been reported for L. brevis strains from Chinese traditional paocai [30]. Once identified, candidate probiotic strains would then be administered to both normal and inflammatory bowel disease-model animals (i.e.…”

Section: Testing the Hypothesismentioning

confidence: 98%

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Probiotics function mechanistically as delivery vehicles for neuroactive compounds: Microbial endocrinology in the design and use of probiotics

2011

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I hypothesize here that the ability of probiotics to synthesize neuroactive compounds provides a unifying microbial endocrinology-based mechanism to explain the hitherto incompletely understood action of commensal microbiota that affect the host's gastrointestinal and psychological health. Once ingested, probiotics enter an interactive environment encompassing microbiological, immunological, and neurophysiological components. By utilizing a trans-disciplinary framework known as microbial endocrinology, mechanisms that would otherwise not be considered become apparent since any candidate would need to be shared among all three components. The range of neurochemicals produced by probiotics includes neurochemicals for which receptor-based targets on immune and neuronal elements (intestinal and extra-intestinal) have been well characterized. Production of neurochemicals by probiotics therefore allows for their consideration as delivery vehicles for neuroactive compounds. This unifying microbial endocrinology-based hypothesis, which may facilitate the selection and design of probiotics for clinical use, also highlights the largely unrecognized role of neuroscience in understanding how microbes may influence health.

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Section: Testing the Hypothesismentioning

confidence: 98%

Section: Testing the Hypothesismentioning

confidence: 98%

Probiotics function mechanistically as delivery vehicles for neuroactive compounds: Microbial endocrinology in the design and use of probiotics

2011

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“…식품이나 기능성식품, 의약품 등에 GABA의 이용 가치를 높이기 위한 균주의 개량과 발효공정개발이 활발히 진행되고 있다. 또한 GABA를 생성하는 유산균을 이용하여 발효식품인 포도주, 간장, sufu, 식초, 증류주, 발효야채, 육제품, 막걸리, paocai, 피클, 김치 및 젓갈 등에 기능성을 강화하고 상품가치 를 높이기 위한 노력을 경주하고 있다 [12,13,15,17,25,29,41,45,48].…”

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Production of gamma-Aminobutyric Acid (GABA) by Lactobacillus plantarum subsp. plantarum B-134 Isolated from Makgeolli, Traditional Korean Rice Wine

이현주¹,

손재영²,

이상재³

et al. 2017

Journal of Life Science

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This study is to isolate and identify γ-amino butyric acid (GABA) producing lactic acid bacteria (LAB) from Makgeolii, traditional Korean rice wine and then establish the optimal culture conditions for GABA production. Sixty four LAB from Makgeolli were isolated according to the characteristics of the shape and color of the colony grown on MRS agar plate. The GABA production of the isolated strain cultured in MRS broth contained 1% MSG (mono-sodium glutamate) were determined and evaluated by TLC and HPLC analysis. Strain B-134 was selected for highest GABA production. From the analysis of 16S rRNA and glutamate decarboxylase B (gadB) gene sequences, strain B-134 was tentatively identified as a Lactobacillus plantarum subsp. plantarum B-134. Effects of culture parameters, including glutamic acid level, culture temperature, NaCl level, and pH on GABA production were investigated for culture optimization. The optimum culture condition for GABA production by B-134 were culture temperature of 37℃, pH of 5.7, NaCl content of 0% (w/v) and MSG content of 3% (w/v), which produced 25 mM of GABA during cultivation time of 48 hr. From these results, strain B-134 is expected to be utilized as useful microorganisms for GABA-enriched health beneficial food.

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“…We previously screened a high GABA-producer L. brevis NCL912 [15] and developed a fed-batch fermentation process for the efficient synthesis of GABA [19]. However, more surplus glucose and L-glutamate remained in the broth when the fermentation was ended [19].…”

Section: Introductionmentioning

confidence: 98%

Separation of gamma-aminobutyric acid from fermented broth

Qiu

Chen

2011

J Ind Microbiol Biotechnol

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Gamma-aminobutyric acid (GABA) is a non-proteinaceous amino acid that is widely distributed in nature and acts as the major inhibitory neurotransmitter in the mammalian brain. This study aimed to find a separation method for getting high-purity GABA from a fermented broth. Firstly, a fermented broth with a high content of GABA (reaching 997 ± 51 mM) was prepared by fermentation with Lactobacillus brevis NCL912. GABA purification was conducted by successive centrifugation, filtration, decoloration, desalination, ion-exchange chromatography (IEC), and crystallization. Inorganic salt (Na₂SO₄) was removed from the both by desalination with 70% ethanol solution. A ninhydrin test strip was designed for the real-time detection of GABA during IEC. The recovery rate for the whole purification process was about 50%. The purified product was characterized by thin-layer chromatography and HPLC, and its purity reached 98.66 ± 2.36%.

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A high γ-aminobutyric acid-producingLactobacillus brevis isolated from Chinese traditionalpaocai

Cited by 86 publications

References 23 publications

Probiotics function mechanistically as delivery vehicles for neuroactive compounds: Microbial endocrinology in the design and use of probiotics

Probiotics function mechanistically as delivery vehicles for neuroactive compounds: Microbial endocrinology in the design and use of probiotics

Production of gamma-Aminobutyric Acid (GABA) by Lactobacillus plantarum subsp. plantarum B-134 Isolated from Makgeolli, Traditional Korean Rice Wine

Separation of gamma-aminobutyric acid from fermented broth

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