2015 28th SIBGRAPI Conference on Graphics, Patterns and Images 2015
DOI: 10.1109/sibgrapi.2015.33
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A Highly Accurate Level Set Approach for Segmenting Green Microalgae Images

Abstract: Abstract-We present a method for segmenting 2D microscopy images of freshwater green microalgae. Our approach is based on a specialized level set method, leading to efficient and highly accurate algae segmentation. The level set formulation of our problem allows us to generate an algae's boundary curve as the result of an evolving level curve, based on computed background and algae regions in a given image. By characterizing the distributions of image intensity values in local regions, we are able to automatic… Show more

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Cited by 8 publications
(4 citation statements)
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“…This robust technique discriminates green microalgae species through chemotaxonomy analysis after revealing a mass range from 0.4 to 2.0 kDa, making it a suitable and alternative method for application in algal taxonomic studies. Some efforts have been channeled towards developing computational support for taxonomical classification of green microalgae species, especially in the Selenastraceae family [80]. In this context, Borges et al [81] described a specialized methodology for segmenting 2D microscopy digital images of freshwater green microalgae.…”
Section: Bioethanol and Biomethanementioning
confidence: 99%
“…This robust technique discriminates green microalgae species through chemotaxonomy analysis after revealing a mass range from 0.4 to 2.0 kDa, making it a suitable and alternative method for application in algal taxonomic studies. Some efforts have been channeled towards developing computational support for taxonomical classification of green microalgae species, especially in the Selenastraceae family [80]. In this context, Borges et al [81] described a specialized methodology for segmenting 2D microscopy digital images of freshwater green microalgae.…”
Section: Bioethanol and Biomethanementioning
confidence: 99%
“…In [58], due to the importance of green microalgae for giving signs to deterioration of ecological conditions, a segmentation algorithm of green microalgae images is proposed. In this approach, eigenvalues of the image are found first, then computation of multivariant Gaussian distribution parameters for algae and background is performed, before thresholding to get a binary image.…”
Section: A Threshold Based Segmentation (Tbs)mentioning
confidence: 99%
“…Object detection procedures using microscopy images rely on both segmentation and bounding box detection. Previous work on microorganism segmentation includes methods such as thresholding, gradient-based, deformable models and feature-based approaches (Verikas et al, 2012;Kloster et al, 2014;Borges et al, 2015;Rojas Camacho et al, 2017). At best, these methods are semi-automatic and require manual fine-tuning of parameters, which is impractical.…”
Section: Introductionmentioning
confidence: 99%