2018
DOI: 10.2323/jgam.2017.11.004
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A highly efficient targeted recombination system for engineering linear chromosomes of industrial bacteria <i>Streptomyces</i>

Abstract: Soil bacteria Streptomyces are the most important producers of secondary metabolites, including most known antibiotics. These bacteria and their close relatives are unique in possessing linear chromosomes, which typically harbor 20 to 30 biosynthetic gene clusters of tens to hundreds of kb in length. Many Streptomyces chromosomes are accompanied by linear plasmids with sizes ranging from several to several hundred kb. The large linear plasmids also often contain biosynthetic gene clusters. We have developed a … Show more

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