2003
DOI: 10.1016/s0264-410x(03)00278-0
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A highly infective Plasmodium vivax strain adapted to Aotus monkeys: Quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development

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Cited by 41 publications
(18 citation statements)
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“…1). The gDNA (extracted using a Wizard Genomic purification kit; Promega, Madison, USA) and cDNA (synthesised with SuperScript III enzyme (RT+) (Invitrogen, Carlsbad, USA) samples from P. vivax VCG-I strain schizont-stage enriched parasites (propagated and obtained as previously described [37, 38]) were used as template in 25 μl PCR reactions containing 1× KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Woburn, MA, USA), 0.3 μM primers and DNAse-free water. Temperature cycling for PCR involved a denaturing step of 95 °C for 5 min, followed by 35 cycles of 98 °C for 20 s, Tm °C (Table 1) for 15 s and 72 °C for 30 s or 1 min and 30 s depending on product size.…”
Section: Methodsmentioning
confidence: 99%
“…1). The gDNA (extracted using a Wizard Genomic purification kit; Promega, Madison, USA) and cDNA (synthesised with SuperScript III enzyme (RT+) (Invitrogen, Carlsbad, USA) samples from P. vivax VCG-I strain schizont-stage enriched parasites (propagated and obtained as previously described [37, 38]) were used as template in 25 μl PCR reactions containing 1× KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Woburn, MA, USA), 0.3 μM primers and DNAse-free water. Temperature cycling for PCR involved a denaturing step of 95 °C for 5 min, followed by 35 cycles of 98 °C for 20 s, Tm °C (Table 1) for 15 s and 72 °C for 30 s or 1 min and 30 s depending on product size.…”
Section: Methodsmentioning
confidence: 99%
“…Since all studies orientated towards developing a rational approach for synthetic vaccines have been carried out in Aotus monkey populations which are highly susceptible to developing human P. falciparum [6] and P. vivax malaria [82], a deep and thorough analysis of their immune system molecules has been undertaken in our institute.…”
Section: Aotus Monkey Immunogeneticsmentioning
confidence: 99%
“…These conserved HABPs were synthesised in polymeric form and used to immunise hundreds of Aotus monkeys (primates which are highly susceptible to developing human malaria) [17,18]. However, conserved HABPs were immunologically silent, since no immune responses were elicited against anyone of them nor protection was induced upon immunisation.…”
Section: Introductionmentioning
confidence: 99%