This study investigates the water-saving and emission-reduction effects of biofilm technology on the pond culture of largemouth bass (Micropterus pallidus) and Japanese eel (Anguilla japonica) using a combination of biofilm water purification grids and a complex microbial preparation. The results show that during the 150-day largemouth bass aquaculture trial, the TN, TAN, TP, nitrite, and LP in the treatment group were significantly lower than those in the control group by 26.2%, 74.7%, 53.9%, 30.7%, and 59.1% (p < 0.01), respectively. During the 145-day aquaculture trial of Japanese eel, the TN, TAN, and TP levels in the treatment group were significantly lower than those in the control group by 30.1%, 68.6%, and 18.7% (p < 0.01), respectively. The nitrite and COD levels were also significantly lower in the treatment group than in the control group by 18.3% and 16.0% (p < 0.05). In addition, largemouth bass and Japanese eel tailwater nitrogen and phosphorus discharges were significantly reduced and culture yields were significantly increased. This biofilm pond culture technology has advantages such as low cost, water saving and emission reduction, increased production, ease of operation, and a wide range of applications.