2-((3-methylthiophen-2-yl)methyleneamino)benzenethiol (Probe 1) has been synthesized and successfully applied for the selective recognition of mercury metal ion and utilized as a fluorescence turn-on sensor for Hg 2+ ion detection via chelation enhanced fluorescence (CHEF). The mechanism of the interaction of Probe 1 with the metal ion has been inculcated by the absorption, emission, 1 H-NMR, MALDI-TOF Mass analysis that intimate the favourable coordination of Hg 2+ metal ion by the mercapto unit. The 2:1 stoichiometry of the sensor complex 1 + Hg 2+ was calculated from the Job's plot based on UV-Vis absorption spectra. The binding constant (log β 2 ) of the 1+Hg 2+ complex was found 13.36 by Hill Plot. The limit of detection (LOD) was also calculated from the fluorescence emission titration that was found 20 µM. Moreover the density functional theory (DFT) studies were investigated for the 1+Hg 2+ binding mechanism. Cyclic Voltammograms were also satisfied the Hg 2+ binding with probe 1.
Introduction:Many human disorders caused by the exposure of the toxic metal ion: like Mercury, Lead, Cadmium, Silver. Many efforts devoted to the development of fluorogenic and colorimetric sensor for the highly toxic mercury ion. Mercury metal ion is highly toxic, non-biodegradable 1 and hazardous in nature 2-4 , toxic for human, including brain, kidney and lung damage. The results of mercury poisoning in several diseases, including Acrodynia, and Minamata disease. The toxicity of Hg 2+ in human caused by the easily coordination with biological ligands such as proteins, DNA and enzymes due to its affinity towards thiol group. Its significant presence hazards to public health because of its presence in drinking water. Therefore, the development of rapid, cost-effective and enzyme-free colorimetric sensors for the easy and fast detection of toxic metal ions by the naked