A highly selective and sensitive biosensor for dopamine based on a surface molecularly imprinted layer to coordinate nano-interface functionalized acupuncture needle

Xu, Caihong; Gu, Chunchuan; Xiao, Qi; Chen, Jiandan; Yin, Zheng‐Zhi; Liu, Hongying; Fan, Kai; Li, Lihua

doi:10.1016/j.cej.2022.135203

Cited by 27 publications

(15 citation statements)

References 44 publications

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“…The main focus was to achieve low detection limits, due to the fact that concentration of DA is extremely low in patients with neurological diseases [ 39 ]. In this context, PC-12 cell lines play an important role in the detection of DA in vivo, with several optical [ 40 , 41 , 42 ], electrochemical biosensing systems, fixed potential amperometry [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] and voltametric ones [ 52 , 53 , 54 ], and electrical devices [ 55 , 56 ] being investigated, with great potential for diagnostic purposes being demonstrated.…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

“…Several electrochemical biosensors were reported for DA detection in PC-12 cells, most of them using fixed potential amperometry [ 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] and voltammetry [ 52 , 53 , 54 ].…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

“…One voltametric biosensor uses a 5-nm-thick poly-celestine blue (CB)-modified glassy carbon electrode (GCE), with a detection limit of 1.2 nM with successful application for DA detection released from PC-12 lines under nicotine stimulation [ 52 ], while the other is based on molecularly imprinted layers of 4-mercaptophenylboronic acid (4-MPBA) and the polymer acid chrome blue K, which is polymerized around the DA molecule to achieve high selectivity toward DA. The later sensor had a detection limit of 140 nM [ 53 ]. The lowest detection limit of voltametric biosensors was achieved by a graphene quantum dots/multiwalled carbon nanotubes (GQDs-MWCNTs)-based biosensor with excellent selectivity for DA and a detection limit of 0.87 nM using DPV [ 54 ].…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

“…Almost all electrochemical biosensors were successfully applied for DA detection released from PC-12 cells under K + [ 44 , 45 , 47 , 50 , 51 , 53 ] or nicotine [ 52 ] stimulation. An important applicability was achieved by the amperometric biosensor in [ 47 ] which was able to evaluate the effects of antipsychotic drug (aripiprazole) on the dopamine release from cells treated with high K + and by the paper-based one in [ 46 ] which monitored the DA released from damaged PC12 cells induced by amyloid-beta peptide (Aβ 25–35 ) and cell intervene models protected by curcumin and marrow mesenchymal stem cells.…”

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

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PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

et al. 2022

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PC-12 cells have been widely used as a neuronal line study model in many biosensing devices, mainly due to the neurogenic characteristics acquired after differentiation, such as high level of secreted neurotransmitter, neuron morphology characterized by neurite outgrowth, and expression of ion and neurotransmitter receptors. For understanding the pathophysiology processes involved in brain disorders, PC-12 cell line is extensively assessed in neuroscience research, including studies on neurotoxicity, neuroprotection, or neurosecretion. Various analytical technologies have been developed to investigate physicochemical processes and the biosensors based on optical and electrochemical techniques, among others, have been at the forefront of this development. This article summarizes the application of different biosensors in PC-12 cell cultures and presents the modern approaches employed in neuronal networks biosensing.

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Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

Section: Metal Ions and Small Molecules Detectionmentioning

confidence: 99%

See 2 more Smart Citations

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

et al. 2022

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“…These methods show high sensitivity and selectivity but require sophisticated and expensive equipment, time-consuming procedures, and skilled operators. Electrochemical methods represent an interesting alternative thanks to their unique advantages, such as their low cost, rapid response, simple operation, and capability for in situ detection [ 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine

et al. 2022

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The regulation of nervous and cardiovascular systems and some brain-related behaviors, such as stress, panic, anxiety, and depression, are strictly dependent on the levels of the main catecholamines of clinical interest, dopamine (DA), epinephrine (EP), and norepinephrine (NEP). Therefore, there is an urgent need for a reliable sensing device able to accurately monitor them in biological fluids for early diagnosis of the diseases related to their abnormal levels. In this paper, we present the first tyrosinase (Tyr)-based biosensor based on chitosan nanoparticles (ChitNPs) for total catecholamine (CA) detection in human urine samples. ChitNPs were synthetized according to an ionic gelation process and successively characterized by SEM and EDX techniques. The screen-printed graphene electrode was prepared by a two-step drop-casting method of: (i) ChitNPS; and (ii) Tyr enzyme. Optimization of the electrochemical platform was performed in terms of the loading method of Tyr on ChitNPs (nanoprecipitation and layer-by-layer), enzyme concentration, and enzyme immobilization with and without 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linking agents. The Tyr/EDC-NHS/ChitNPs nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, as evidenced by its high biocatalytic activity toward the oxidation of DA, EP, and NEP to the relative o-quinone derivatives electrochemically reduced at the modified electrode. The resulting Tyr/EDC-NHS/ChitNPs-based biosensor performs interference-free total catecholamine detection, expressed as a DA concentration, with a very low LOD of 0.17 μM, an excellent sensitivity of 0.583 μA μM−1 cm−2, good stability, and a fast response time (3 s). The performance of the biosensor was successively assessed in human urine samples, showing satisfactory results and, thus, demonstrating the feasibility of the proposed biosensor for analyzing total CA in physiological samples.

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Percutaneous Wearable Biosensors: A Brief History and Systems Perspective

Peterson,

Shukla,

Daniele

2024

Advanced Sensor Research

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Wearable biosensors are envisioned to disrupt both delivery and accessibility of healthcare by providing real‐time, continuous monitoring of informative and predictive physiological markers in convenient, user‐friendly, and portable designs. In recent years, there has been myriad demonstrations of biosensor‐integrated clothing and skin‐borne biosensor patches, enabled by device miniaturization, reduced power consumption, and new biosensing chemistries. Despite these impressive demonstrations, most consumer‐grade wearables have been limited to biophotonic and biopotential sensing methods to extrapolate information such as pulse, blood oxygenation, and electrocardiograms. The only commercial example of wearable electrochemical sensing methods is for glucose monitoring. However, there is a growing interest in developing percutaneous biosensors for monitoring in interstitial fluid (ISF), which offers direct access to popular analytes such as glucose, lactate, and urea, as well as new targets like hormones, antibodies, and even medications. Herein, a brief context for the current status of wearable biosensors is provided and assess the major engineering successes and pitfalls of percutaneous biosensors over the past five years, with a view to identifying areas for further developments that will enable deployable, clinical‐ or consumer‐grade systems.

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A highly selective and sensitive biosensor for dopamine based on a surface molecularly imprinted layer to coordinate nano-interface functionalized acupuncture needle

Cited by 27 publications

References 44 publications

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine

Percutaneous Wearable Biosensors: A Brief History and Systems Perspective

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