A highly selective and sensitive in vivo fluorosensor for zinc(ii) without cytotoxicity

Abstract

A highly selective and sensitive fluorescent Zn(2+) sensor, 2,6-bis(2-hydroxy-benzoic acid hydrazide)-4-methylphenol (1), was designed and synthesized. In aqueous THF (4 : 6 v/v) ligand 1 induces a 2 : 1 complex formation with respect to Zn(2+) at physiological pH. This probe features visible light excitation(390 nm) and emission (490 nm) profiles, excellent selectivity responses for Zn(2+)over other competing biological metal ions with K(d) < 1 pM(2), LOD < 1 ng L(-1) and about 680 fold enhancement in fluorescent intensity upon Zn(2+) binding. It also exhibits cell permeability and intracellular Zn(2+) sensing in A375 human melanoma cancer cell.

Selective Recognition of Zn2+ Ion Using 2,4‐Bis(2‐pyridyl)‐5‐(4‐pyridyl)imidazole: Spectra and Molecular Structure

“…Among the several methods available for determination of Zn 2+ ion, fluorescence has become more useful and popular method in environmental chemistry, medicine and biology. The most common fluorescent probes for detection of Zn 2+ ion are coumarin, Schiff bases, calixarene, hydroxyflavone 8‐hydroxyquinoline, and imidazoline . Prime reason for lesser development of zinc(II) specific sensors is the poor coordination of ligands towards bivalent zinc, in comparison with other common transition metal ions.…”

The Application of Silver Nanoclusters to Sensing, Cell Imaging and Construction of Molecular Logic Gates

“…This selectivity of Co 2+ over other relevant metal ions is represented with a bar diagram (inset (a) Figure ). The Limit of Detection (LOD) of AgNC was determined by 3σ method ,. In this method LOD=3σ/m where ‘σ’ is the standard deviation of blank (1.20) and ‘m’ is the slope of the fluorescence intensity vs [Co 2+ ] plot, inset (b) Figure .…”

Rhodamine‐Based Chromo‐/Fluorogenic Dual Signalling Probe for Selective Recognition of HgII with Potential Applications for INHIBIT Logic Devices and Cell‐Imaging Studies

“…[17] Also, this study has revealed new potential applications in the development of new-generation molecular electronic devices, a molecular INHIBIT logic gate, in which Hg 2+ and Iare used as chemical inputs and the fluorescence signal as output. [17] Also, this study has revealed new potential applications in the development of new-generation molecular electronic devices, a molecular INHIBIT logic gate, in which Hg 2+ and Iare used as chemical inputs and the fluorescence signal as output.…”