A highly selective electrochemical assay based on the Sakaguchi reaction for the detection of protein arginine methylation state

He, Mengyuan; Guo, Jiarong; Yang, Jin Min; Yang, Yang; Zhao, Songyan; Xu, Qiao; Wei, Tiangxiang; Ferraris, Davide M.; Gao, Tao; Guo, Zhigang

doi:10.1016/j.elecom.2020.106808

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…XPS and FT-IR characterizations verified that the existence of amino, carboxyl, and guanidinium groups at the edge of LAAM GUA-CDs. On the basis of the result of Sakaguchi reaction, [33,34] each LAAM GUA-CDs structure contained an average of 8 guanidinium groups. According to the result of ninhydrin reaction, [35][36][37] each LAAM GUA-CDs structure contains 12 amino acid groups (Figure S3a-d, Supporting Information).…”

Section: Synthesis and Characterization Of Large Amino Acid Mimicking...mentioning

confidence: 99%

Carbon Dots with Guanidinium and Amino Acid Functional Groups for Targeted Small Interfering RNA Delivery toward Tumor Gene Therapy

Chang

et al. 2023

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Small interfering RNA (siRNA)‐based gene therapy represents a promising strategy for tumor treatment. Novel gene vectors that can achieve targeted delivery of siRNA to the tumor cells without causing any side effects are urgently needed. To this end, the large amino acid mimicking carbon dots with guanidinium functionalization (LAAM GUA‐CDs) are designed and synthesized by choosing arginine and dopamine hydrochloride as precursors. LAAM GUA‐CDs can load siRNA through the multiple hydrogen bonds between their guanidinium groups and phosphate groups in siRNA. Meanwhile, the amino acid groups at the edges of LAAM GUA‐CDs endow them the capacity to target tumors. After loading siBcl‐2 as a therapeutic agent, LAAM GUA‐CDs/siBcl‐2 has a high tumor inhibition rate of up to 68%, which is twice more than that of commercial Lipofectamine 2000. Furthermore, LAAM GUA‐CDs do not cause side effect during antitumor treatment owing to their high tumor‐targeting ability, thus providing a versatile strategy for tumor‐targeted siRNA delivery and cancer therapy.

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Section: Synthesis and Characterization Of Large Amino Acid Mimicking...mentioning

confidence: 99%

Carbon Dots with Guanidinium and Amino Acid Functional Groups for Targeted Small Interfering RNA Delivery toward Tumor Gene Therapy

Chang

et al. 2023

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“…The method based on chromatography , is critical to select the appropriate mobile phase; however, most mobile phase additives lack high specificity and sensitivity to their target. In addition, the electrochemical assay depends on specific chemical reactions and cannot achieve a direct identification effect and research based on fluorescent sensors always needs the design of complex probes. Moreover, these methods are always based on the bulk solution analysis and are unable to obtain information at the single molecular level.…”

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confidence: 99%

Recognition Receptor for Methylated Arginine at the Single Molecular Level

et al. 2023

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Among the various types of post-translational modifications (PTMs), methylation is the simple functionalized one that regulates the functions of proteins and affects interactions of protein–protein and protein–DNA/RNA, which will further influence diverse cellular processes. The methylation modification has only a slight effect on the size and hydrophobicity of proteins or peptides, and it cannot change their net charges at all, so the methods for recognizing methylated protein are still limited. Here, we designed a recognition receptor consisting of a α-hemolysin (α-HL) nanopore and polyamine decorated γ-cyclodextrin (am8γ-CD) to differentiate the methylation of peptide derived from a heterogeneous nuclear ribonucleoprotein at the single molecule level. The results indicate that the modification of a methyl group enhances the interaction between the peptide and the recognition receptor. The results of molecular simulations were consistent with the experiments; the methylated peptide interacts with the receptor strongly due to the more formation of hydrogen bonds. This proposed strategy also can be used to detect PTM in real biological samples and possesses the advantages of low-cost and high sensitivity and is label-free. Furthermore, the success in the construction of this recognition receptor will greatly facilitate the investigation of pathogenesis related to methylated arginine.

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