A highly selective near-infrared fluorescent probe for imaging H<sub>2</sub>Se in living cells and in vivo

Kong, Fanming; Ge, Lihong; Pan, Xiaohong; Xu, Kehua; Liu, Xiaojun; Tang, Bo

doi:10.1039/c5sc03471j

Cited by 72 publications

(49 citation statements)

References 49 publications

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“…19). 36 Initially, the free chemosensor is non-fluorescent due to the heavy atom effect of Se. However, after the addition of H 2 Se, the chemosensor undergoes a ca.…”

Section: Fluorescent Chemosensors For Reactive Sulfur Species (Rss)mentioning

confidence: 99%

Fluorescent chemosensors: the past, present and future

et al. 2017

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Fluorescent chemosensors for ions and neutral analytes have been widely applied in many diverse fields such as biology, physiology, pharmacology, and environmental sciences. The field of fluorescent chemosensors has been in existence for about 150 years. In this time, a large range of fluorescent chemosensors have been established for the detection of biologically and/or environmentally important species. Despite the progress made in this field, several problems and challenges still exist. This tutorial review introduces the history and provides a general overview of the development in the research of fluorescent sensors, often referred to as chemosensors. This will be achieved by highlighting some pioneering and representative works from about 40 groups in the world that have made substantial contributions to this field. The basic principles involved in the design of chemosensors for specific analytes, problems and challenges in the field as well as possible future research directions are covered. The application of chemosensors in various established and emerging biotechnologies, is very bright.

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“…19). 36 Initially, the free chemosensor is non-fluorescent due to the heavy atom effect of Se. However, after the addition of H 2 Se, the chemosensor undergoes a ca.…”

Section: Fluorescent Chemosensors For Reactive Sulfur Species (Rss)mentioning

confidence: 99%

Fluorescent chemosensors: the past, present and future

et al. 2017

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“…Compound 138 was a H 2 Se sensor, according to the fact that the Se−N in benzoselenadizole moiety could be specifically destroyed by H 2 Se through nucleophilic addition. 185 Because of the heavy atom effect of Se, the fluorescence of 138 was effectively quenched. As expected, the reaction of the sensor with H 2 Se yielded a diamine product ( Figure 56) and recovered the fluorescence of the fluorophore (735 nm, λ ex = 688 nm).…”

Section: Sensors Based On Modulating the Electron-donating Ability Ofmentioning

confidence: 99%

Recent Development of Chemosensors Based on Cyanine Platforms

et al. 2016

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The cyanine platforms including cyanine, hemicyanine, and squaraine are good candidates for developing chemosensors because of their excellent photophysical properties, outstanding biocompatibility, and low toxicity to living systems. A huge amount of research work involving chemosensors based on the cyanine platforms has emerged in recent years. This review focuses on the development from 2000 to 2015, in which cyanine, hemicyanine, and squaraine sensors will be separately summarized. In each section, a systematization according to the type of detection mechanism is established. The basic principles about the design of the chemosensors and their applications as bioimaging agents are clearly discussed. In addition, we emphasize the advances that have been made in improving the detection performance through incorporation of the chemosensors into nanoparticles.

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“…However, the role of H 2 Se in selenium compound treatments for cancers has not been elucidated due to the lack of detection methods. In our previous study, we developed a specific fluorescent probe for the real-time monitoring of H 2 Se in living cells and in vivo 4 (Fig. 1A).…”

Section: Introductionmentioning

confidence: 99%

H₂Se Induces Reductive Stress in HepG2 Cells and Activates Cell Autophagy by Regulating the Redox of HMGB1 Protein under Hypoxia

Pan¹,

Song²,

Wang

et al. 2019

Theranostics

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Rationale: Selenium has been shown to have chemotherapeutic effects against cancer. However, the anti-cancer mechanism of selenium is not fully understood, and the role of hydrogen selenide (H 2 Se), which is a common metabolite of dietary selenium compounds, has not been elucidated due to the lack of detection methods. In this study, we revealed a new anti-cancer mechanism of selenite with the help of a H 2 Se fluorescent probe. Methods: HepG2 cells were cultured under a simulated tumor hypoxic microenvironment. The H 2 Se and H 2 O 2 levels were detected by fluorescent probes in living cells and in mice. Autophagic and apoptotic proteins were detected by Western blotting. The redox of HMGB1 protein were analyzed by non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis. Results: After pharmacological doses of Na 2 SeO 3 treatment of HepG2 cells under hypoxic conditions, high levels of H 2 Se were produced before cell death. The H 2 Se accumulation resulted in reductive stress instead of oxidative stress, which was induced by Na 2 SeO 3 treatment under normoxic conditions. Furthermore, H 2 Se targeted the HMGB1 protein and induced cell autophagy. H 2 Se could interrupt the disulfide bond in HMGB1 and promote its secretion. The reduced HMGB1 outside the cells stimulated cell autophagy by inhibiting the Akt/mTOR axis. Here, autophagy played a dual role, i.e., mild autophagy inhibited apoptosis, while excessive autophagy led to autophagy-associated cell death. Conclusions: These results show that H 2 Se plays a key role during HepG2 cell death induced by selenite. Our findings reveal a new anti-cancer mechanism of selenite and provide a new research area for selenium studies.

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A highly selective near-infrared fluorescent probe for imaging H₂Se in living cells and in vivo

Abstract: The first near-infrared fluorescent probe to detect H2Se with high sensitivity was explored in living cells and in mice.

Cited by 72 publications

References 49 publications

Fluorescent chemosensors: the past, present and future

Fluorescent chemosensors: the past, present and future

Recent Development of Chemosensors Based on Cyanine Platforms

H₂Se Induces Reductive Stress in HepG2 Cells and Activates Cell Autophagy by Regulating the Redox of HMGB1 Protein under Hypoxia

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A highly selective near-infrared fluorescent probe for imaging H2Se in living cells and in vivo

Abstract: The first near-infrared fluorescent probe to detect H2Se with high sensitivity was explored in living cells and in mice.

Cited by 72 publications

References 49 publications

Fluorescent chemosensors: the past, present and future

Fluorescent chemosensors: the past, present and future

Recent Development of Chemosensors Based on Cyanine Platforms

H2Se Induces Reductive Stress in HepG2 Cells and Activates Cell Autophagy by Regulating the Redox of HMGB1 Protein under Hypoxia

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Product

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A highly selective near-infrared fluorescent probe for imaging H₂Se in living cells and in vivo

H₂Se Induces Reductive Stress in HepG2 Cells and Activates Cell Autophagy by Regulating the Redox of HMGB1 Protein under Hypoxia