A highly selective NIR ratiometric fluorescent probe for in situ detection of metabolized hydrazine in living cells

An AIE-active tetra-aryl imidazole-derived chemodosimeter for turn-on recognition of hydrazine and its bioimaging in living cells

“…At present, most fluorescent probes for the detection of hydrazine are based on the following reaction mechanisms: nucleophilic substitution of hydrazine for methyl-enemalononitrile, [11][12][13][14] condensation of hydrazine and aldehydes or ketones, 15 deprotection of acetyl groups and their derivatives, [16][17][18] and hydrazine-induced cyclization or ringopening reactions. [19][20][21][22] Unfortunately, the fluorescence of these hydrazine identification probes is easily quenched in aqueous solutions or in the solid state due to the aggregationcaused quenching (ACQ) effect, which greatly limits their application.…”